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An EST-SSR-based linkage map for Persea americana Mill. (avocado)

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Abstract

Recent enhancement of the pool of known molecular markers for avocado has allowed the construction of the first moderately dense genetic map for this species. Over 300 SSR markers have been characterized and 163 of these were used to construct a map from the reciprocal cross of two Florida cultivars 'Simmonds' and 'Tonnage'. One hundred thirty-five primer pairs amplified 163 usable loci with 20 primer pairs amplifying more than one locus. 'Tonnage' was heterozygous for 152 (93%) loci, whereas 'Simmonds' was heterozygous for 64 (39%). Null alleles were identified at several loci. Linkage maps were produced for both reciprocal crosses and combined to generate a composite linkage map for the F1 population of 715 individuals. The composite map contains 12 linkage groups. Linkage groups ranged in size from 157.3 cM (LG2) to 2.4 cM (LG12) and the number of loci mapped per group ranged from 29 (LG1) to two (LG12). The total map length was 1,087.4 cM. Only seven markers were observed to have segregation distortion (α ≤ 0.05) across both sub-composite (reciprocal) maps. Phenotypic data from traits of horticultural interest are currently being collected on this population with the ultimate goal of identifying useful quantitative trait loci and the development of a marker-assisted selection program.

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Author notes

  1. James W. Borrone & Margarita Mauro-Herrera

    Present address: Department of Botany, Oklahoma State University-Stillwater, 104 Life Sciences East, Stillwater, OK, 74078-3013, USA

Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Subtropical Horticulture Research Station, National Germplasm Repository, 13601 Old Cutler Road, Miami, FL, 33158, USA

    James W. Borrone, J. Steven Brown, Cecile L. Tondo, Margarita Mauro-Herrera, David N. Kuhn, Helen A. Violi, Robert T. Sautter & Raymond J. Schnell

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  1. James W. Borrone
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  2. J. Steven Brown
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  3. Cecile L. Tondo
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  4. Margarita Mauro-Herrera
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  5. David N. Kuhn
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  6. Helen A. Violi
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  7. Robert T. Sautter
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  8. Raymond J. Schnell
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Corresponding author

Correspondence to Raymond J. Schnell.

Additional information

Communicated by: F. Gmitter

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Borrone, J.W., Brown, J.S., Tondo, C.L. et al. An EST-SSR-based linkage map for Persea americana Mill. (avocado). Tree Genetics & Genomes 5, 553–560 (2009). https://doi.org/10.1007/s11295-009-0208-y

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  • DOI: https://doi.org/10.1007/s11295-009-0208-y

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