Tree Genetics & Genomes

, 5:181 | Cite as

Role of genomics in the potential restoration of the American chestnut

  • Nicholas WheelerEmail author
  • Ronald Sederoff
Original Paper


The development of genomic tools will enhance traditional tree breeding technologies leading to more certain and timely recovery of the American chestnut, a keystone heritage tree of the eastern United States. Major efforts are being made in gene discovery, genetic marker development, construction of a BAC-based physical map, and DNA transformation technology. A strategy of map-based cloning, association genetics, and genetic engineering, combined with traditional and marker-assisted backcross breeding is proposed for the long-term genetic restoration of this iconic tree species.


Genomics Chestnut Restoration 


  1. Allen TD, Dawe AL, Nuss DL (2003) Use of cDNA microarrays to monitor transcriptional responses of the chestnut blight fungus Cryphonectria parasitica to infection by virulence-attenuating hypoviruses. Eukaryotic Cell 2003:1253–1265CrossRefGoogle Scholar
  2. Anagnostakis SL (1977) Vegetative incompatibility in Endothia parasitica. Exp Mycol 1:306–316CrossRefGoogle Scholar
  3. Anagnostakis SL (2001) The effect of multiple importations of pests and pathogens on a native tree. Biological Invasions 3:245–254CrossRefGoogle Scholar
  4. Anderson PJ (1914) The morphology and life history of the chestnut blight fungus. Bulletin 7, Pennsylvania Chestnut Tree Blight Commission, Harrisburg, Pennsylvania, 44 ppGoogle Scholar
  5. Andrade GM, Nairn CJ, Le HT, Merkle SA (2005) Regeneration of transgenic American Chestnut plants following co-cultivation of embryogenic tissues with Agrobacterium tumefaciens. IUFRO Tree Biotechnology 2005, November 6–11, 2005, Pretoria, South Africa. Abstract No. S7, p 10Google Scholar
  6. Barreneche T, Casasoli M, Russell K, Akkak A, Meddour H, Plomion C, Villani F, Kremer A (2004) Comparative mapping between Quercus and Castanea using simple-sequence repeats (SSRs). Theor Appl Genet 108:558–566PubMedCrossRefGoogle Scholar
  7. Bent AF (1996) Plant disease resistance genes: function meets structure. Plant Cell 8:1757–1771PubMedCrossRefGoogle Scholar
  8. Carraway DT, Wilde HD, Merkle SA (1994) Somatic embryogenesis and gene transfer in American chestnut. J Am Chestnut Found 8(1):29–33Google Scholar
  9. Casasoli M, Mattioni C, Cherubini M, Villani F (2001) A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers. Theor Appl Genet 102:1190–1199CrossRefGoogle Scholar
  10. Casasoli M, Derory J, Morera-Dutrey C, Brendel O, Porth I, Guehl JM, Villani F, Kremer A (2006) Comparison of quantitative trait loci for adaptive traits between oak and chestnut based on an expressed sequence tag consensus map. Genetics 172:533–546PubMedCrossRefGoogle Scholar
  11. Castro MS, Fontes W (2005) Plant defense and antimicrobial peptides. Protein and Peptide Letters 12:11–16CrossRefGoogle Scholar
  12. Dana MdlM, Pintor-Toro JA, Cubero B (2006) Transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents. Plant Physiol 142:722–730CrossRefGoogle Scholar
  13. Davis D (2006) Historical significance of American chestnut to Appalachian culture and ecology. In: Steiner KC and Carlson JE (eds) Restoration of American chestnut to forest lands—Proc of a conference and workshop. May 4–6, 2004, The North Carolina Arboretum. Natural Res Rep NPS/NCR/CUE/NRR-2006/001, National Park ServiceGoogle Scholar
  14. Dekkers JCM, Hospital F (2002) The use of molecular genetics in improvement of agricultural populations. Nat Rev Genet 3:22–32PubMedCrossRefGoogle Scholar
  15. Freinkel S (2007) American chestnut: the life, death, and rebirth of a perfect tree. University of California Press, Berkeley, CAGoogle Scholar
  16. Friedt W, Ordon F (2007) Molecular markers for gene pyramiding and disease resistance breeding in barley. In: Varshney RK, Tuberosa R (eds) Genomics-assisted crop improvement, vol 2: Genomics applications in crops. Springer, pp 81–102Google Scholar
  17. Gonzalez-Martinez SC, Wheeler NC, Ersoz E, Nelson CD, Neale DB (2007) Association genetics in Pinus taeda L. I. Wood property traits. Genetics 175:399–409PubMedCrossRefGoogle Scholar
  18. Grente J (1965) Les formes hypovirulentes d’Endothia parasitica et les espoirs de lutte contre le chancre du chantaingnier. CR Acad Agric France 51:1033–1037Google Scholar
  19. Griffin GJ (2000) Blight control and restoration of the American chestnut. J For 98:22–27Google Scholar
  20. Griffin GJ, Elkins JR, McCurdy D, Griffin SL (2006) Integrated use of resistance, Hypovirulence, and forest management to control blight on American chestnut. In: Steiner KC, Carlson JE (eds) Restoration of American chestnut to forest lands—Proc of a conference and workshop. May 4–6, 2004, The North Carolina Arboretum. Natural Res Rep NPS/NCR/CUE/NRR-2006/001, National Park ServiceGoogle Scholar
  21. Hebard FV (2006) The backcross breeding program of the American chestnut foundation. In: Steiner KC, Carlson JE (eds) Restoration of American chestnut to forest lands—Proc of a conference and workshop. May 4–6, 2004, The North Carolina Arboretum. Natural Res Rep NPS/NCR/CUE/NRR-2006/001, National Park ServiceGoogle Scholar
  22. Hill JM (1994) Wildlife value of Castanea dentata past and present, the historical decline of the chestnut and its future use in restoration of natural areas. In: Double ML, MacDonald WL (eds) Proceedings of the International Chestnut Conference. West Virginia University Press, Morgantown, West Virginia, pp 186–193Google Scholar
  23. Hillel J, Schaap T, Haberfeld A, Jeffreys AJ, Plotzky Y, Cahaner A, Lavi U (1990) DNA fingerprints applied to gene introgression in breeding programs. Genetics 124:783–789PubMedGoogle Scholar
  24. Hospital F, Charcosset A (1997) Marker-assisted introgression of quantitative trait loci. Genetics 147:1469–1485PubMedGoogle Scholar
  25. Hospital F, Chevalet C, Mulsant P (1992) Using markers in gene introgression breeding programs. Genetics 132:1199–1210PubMedGoogle Scholar
  26. Hospital F, Bouchez A, Lecomte L, Causse M, Charcosset A (2002) Use of markers in plant breeding: Lessons from genotype building experiments. Electronic communication 22:05 in Proc. 7th World Cong. Genet. Appl. Livest. Prod., Montpellier, FranceGoogle Scholar
  27. Huang R, Xiang Y, Liu X, Zhang Y, Hu Z, Wang D (2002) Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliv. FEBS Lett 521:87–90PubMedCrossRefGoogle Scholar
  28. Islam-Faridi N, Nelson CD, Banda H, Majid MA, Kubisiak TL, Hebard FV, Sisco PH, Paris RL, Phillips RL (2008) Cytogenetic analysis of a reciprocal translocation in F1 hybrid between American and Chinese chestnuts. Plant and Animal Genomes XVI Conference. Abstract W346. San Diego, CAGoogle Scholar
  29. Jacobs DF (2005) Evaluating the efficiency of carbon sequestration in American chestnut (Castanea dentata), EPRI, Palo Alto, CA: 1011518Google Scholar
  30. Jander G, Norris SR, Rounsley SD, Bush DF, Levin IM, Last RL (2002) Arabidopsis map based cloning in the post genomic era. Plant Physiol 129:440–450PubMedCrossRefGoogle Scholar
  31. Jaynes RA (1994) Reflections. In: Double ML, MacDonald (eds) Proc International Chestnut Conference. West Virginia University Press, MorgantownGoogle Scholar
  32. Kohler A, Rinaldi C, Duplessis S, Baucher M, Geelen D, Duchaussoy F, Meyers B, Boerjan W, Martin F (2008) Genome-wide identification of NBS resistance genes in Populus trichocarpa. Plant Mol Biol 66:619–636PubMedCrossRefGoogle Scholar
  33. Kubisiak TL, Hebard FV, Nelson CD, Zhang J, Bernatzky R, Huang H, Anagnostakis SL, Doudrick RL (1997) Molecular mapping of resistance to blight in an interspecific cross in the genus Castanea. Amer Phytopathological Soc 87:751–759Google Scholar
  34. Lane BG (2002) Oxalate, germins and higher plant pathogens. IUBMB Life 53:67075CrossRefGoogle Scholar
  35. Lecape J-M, Nguyen T-B, Hau B, Giband M (2007) Targeted introgression of cotton fibre quality quantitative trait loci using molecular markers. In: Guimaraes EP, Ruane J, Schert BD, Sonnino A, Dargie JD (eds) Marker-assisted selection: current status and future perspectives in crops, livestock, forestry and fish. FAO, RomeGoogle Scholar
  36. MacDonald WL, Double ML (2006) Hypovirulence: use and limitations as a chestnut blight biological control. In: Steiner KC, Carlson JE (eds) Restoration of American chestnut to forest lands—Proc of a conference and workshop. May 4–6, 2004, The North Carolina Arboretum. Natural Res Rep NPS/NCR/CUE/NRR-2006/001, National Park ServiceGoogle Scholar
  37. MacDonald WL, Fulbright DW (1991) Biological control of chestnut blight: use and limitations of transmissible hypovirulence. Plant Dis 75:656–661Google Scholar
  38. Margulies M, Egholm M et al (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376–380PubMedGoogle Scholar
  39. McHale L, Tan X, Koehl P, Michelmore R (2006) Plant NBS-LRR proteins: adaptable guards. Genome Biology 7:212–227PubMedCrossRefGoogle Scholar
  40. Mehlenbacher SA, Brown RN, Davis JW, Chen H, Bassil NV, Smith DC, Kubisiak TL (2004) RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Theor Appl Genet 108:651–656PubMedCrossRefGoogle Scholar
  41. Merkle SA, Andrade GM, Nairn CJ, Powell WA, Maynard CA (2006) Restoration of threatened species: a noble cause for transgenic trees. Tree Genetics and Genomes 3(2):111–118CrossRefGoogle Scholar
  42. Morgante M, Salamini F (2003) From plant genomics to breeding practice. Curr Opin Biotechnol 14:214–219PubMedCrossRefGoogle Scholar
  43. Neale DB (2007) Genomics to tree breeding and forest health. Curr Opin Genet Dev 17:1–6CrossRefGoogle Scholar
  44. Neale DB, Ingvarsson P (2008) Population, quantitative and comparative genomics of adaptation in forest trees. Curr Opin Plant Biol 11:1–7CrossRefGoogle Scholar
  45. Neale DB, Savolainen O (2004) Association genetics of complex traits in conifers. Trends Plant Sci 9:325–330PubMedCrossRefGoogle Scholar
  46. Paillet FL (2000) Chestnut: history and ecology of a transformed species. J Biogeogr 29:1517–1530CrossRefGoogle Scholar
  47. Polin LD, Liang H, Rothrock RE, Hishii M, Diehl DL (2006) Agrobacterium-mediated transformation of American chestnut [(Castanea dentata Marsh.) Borkh.] somatic embryos. Plant Cell Tissue Organ Cult 84:69–78CrossRefGoogle Scholar
  48. Rafalski A (2002) Applications of single nucleotide polymorphisms in crop genetics. Curr Opin Plant Biol 5:94–100PubMedCrossRefGoogle Scholar
  49. Rieseberg LH, Linder CR, Seiler GJ (1995) Chromosomal and genic barriers to introgression in Helianthus. Genetics 141:1163–1171PubMedGoogle Scholar
  50. Russell EWB (1987) Preblight distribution of Castanea dentata (Marsh) Borkh. Bull Torrey Bot Club 114:183–190CrossRefGoogle Scholar
  51. Russell EWB, Davis RB (2001) Five centuries of changing forest vegetation in the northeastern United States. Plant Ecol 155:1–13CrossRefGoogle Scholar
  52. Salvi S, Tuberosa R (2007) Cloning QTLs in plants. In: Varshney RK, Tuberosa R (eds) Genomics-assisted crop improvement, vol 1: genomics approaches and platforms. Springer, pp 207–226Google Scholar
  53. Saucier JR (1973) Natural range of American chestnut. USDA Forest Service Fact Sheet 230Google Scholar
  54. Sisco PH, Kubisiak TL, Cadasoli M, Barreneche T, Kremer A, Clark C, Sederoff PR, Hebard FV, Villani F (2005) An improved genetic map for Castanea mollissima/Castanea dentata and its relationship to the genetic map of C. sativa. In: Abreu CF, Rosa E, Monteirro AA (eds) Proc. IIIrd Intl. Chestnut Congress. Acta Hort 693:491–495Google Scholar
  55. Stephenson SL, Adams HS, Lipford ML (1991) The present distribution of chestnut in the upland forest communities of Virginia. Bull Torrey Bot Club 118:24–32CrossRefGoogle Scholar
  56. Strauss SH, Bradshaw HD (eds) (2004) The BioEngineered forest: challenges to science and society (Resources for the Future, Washington, DC, 2004). ISBN 1-891853-71-6Google Scholar
  57. Tanksley SD, Ganal MW, Martin GB (1995) Chromosome landing: a paradigm for map based gene cloning in plants with large genomes. TIG 11:63–68PubMedGoogle Scholar
  58. Tenaillon MI, Sawkins MC, Long AD, Gaut RL, Doebley JF, Gaut BS (2001) Patterns of DNA sequence polymorphism along chromosome 1 in maize. Proc Natl Acad Sci 98:9161–9166PubMedCrossRefGoogle Scholar
  59. U.S. Census Bureau (1908) The lumber cut of the United States, 1907. For Products 2:1–53Google Scholar
  60. Verhoeven KJF, Jannink J-L, McIntyre LM (2006) Using mating designs to uncover QTL and the genetic architecture of complex traits. Heredity 96:139–149PubMedCrossRefGoogle Scholar
  61. Welch AJ, Stipanovic AJ, Maynard CA, Powell WA (2007) The effects of oxalic acid on transgenic Castanea dentata callus tissue expressing oxalate oxidase. Plant Sci 172:488–496CrossRefGoogle Scholar
  62. White TL, Adams WT, Neale DB (2007) Forest genetics. CABI Publishing, Cambridge MAGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighUSA

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