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Tree Genetics & Genomes

, Volume 1, Issue 2, pp 69–78 | Cite as

Identification and characterization of transcripts differentially expressed during development of apricot (Prunus armeniaca L.) fruit

  • Filippo Geuna
  • Riccardo Banfi
  • Daniele Bassi
Original Paper

Abstract

The application of the complementary DNA (cDNA)–amplified fragment length polymorphism (AFLP) technique to clone transcripts differentially expressed during fruit development and ripening is reported. Using 34 different primer combinations, 265 cDNA–AFLP bands were found differentially expressed in six fruit developmental stages, with leaf and developing seed as controls. One hundred twenty-five bands were cloned and sequenced and database search allowed to identify genes involved in cell wall, sugar, lipid, organic acids, and protein metabolism, as well as genes participating in hormonal signaling and in signal transduction (51 sequences, 41% of total). Genomic and expression analyses for a group of genes as well as phylogenetic investigations were carried out. cDNA–AFLP profiles have been also compared to the corresponding Northern hybridization data. Several genes are described for the first time in apricot or in other Prunus species. They establish the basis for further investigation of the ripening process in stone fruits.

Keywords

cDNA–AFLP Differential expression Fruit ripening Transcript profiling 

Notes

Acknowledgements

We wish to thank Francesco Salamini and the anonymous referees for helpful suggestions and critical review of the manuscript; Phil Green, Brent Ewing, and David Gordon, University of Washington, Seattle, USA, for providing the Phred/Phrap/Consed suite of programs; Ilaria Mignani, University of Milano, Italy, for the assistance in the analysis of fruits for chemical parameters; and Davide Vivoli for assistance in collecting of plant material. This work was partially funded by a grant from the Italian Ministry of Instruction, University and Research (MIUR) in the framework of research program COFIN 2001. The work has been carried out in compliance with the current Italian laws governing genetic experimentation.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Dipartimento di Produzione Vegetale (DIPROVE)University of MilanoMilanoItaly

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