Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Species-specific detection of the endangered piscivorous cyprinid fish Opsariichthys uncirostris uncirostris, three-lips, using environmental DNA analysis

Abstract

Opsariichthys uncirostris uncirostris is a subspecies of Amur three-lips Opsariichthys uncirostris (Temminck and Schlegel, 1846) and endemic to Japan. This only piscivorous fish among Cyprinidae in Japan, hereafter three-lips, is in a unique ecological situation, that is, the species is endangered in the native habitats such as Lake Biwa water system and Lake Mikata, but on the other hand, three-lips is threatening the other native species as an intra-nation invasive species in introduced habitats. In this study, a species-specific primer–probe set was developed for detecting three-lips using environmental DNA (eDNA) analysis for monitoring the species distribution. Ten out of 11 sites in Futatsugawa River system, Kyusyu, and 15 out of 28 sites in Lake Biwa tested positive. In the Futatsugawa, three-lips was confirmed to occupy both of the lotic sites in the main stem of the river and the lentic sites in the inner part of small ditches. In Lake Biwa, positives were biased to the sites in north basin of the lake than smaller south basin. The PCR amplicons in the positive samples were confirmed to have DNA sequences of the target species, suggesting the accuracy of this species-specific analysis. The eDNA detection system developed in this study will contribute to future follow-up monitoring by providing a cost-effective and less labor-intensive method for determining the distribution of three-lips with dual identities as a threatened and invasive species.

This is a preview of subscription content, log in to check access.

Fig. 1

References

  1. Darling JA, Mahon AR (2011) From molecules to management: adopting DNA-based methods for monitoring biological invasions in aquatic environments. Environ Res 111:978–988

  2. Imamura A (2014) A detailed assessment of the distribution of Opsariichthys uncirostris uncirostris along the shoreline of Lake Biwa. Jpn. J. Conserv Ecol 19:151–158 (in Japanese)

  3. Kurita Y, Nakajima J, Kaneto J, Onikura N (2008) Analysis of the gut contents of the internal exotic fish species Opsariichthys uncirostris uncirostris in the Futatsugawa River, Kyushu Island, Japan. J Facul Agr Kyushu Univ. 53:429–433

  4. Kurita Y, Onikura N, Inui R (2014) Factors affecting the establishment success of the invasive piscivorous chub in small irrigation ditches in northern Kyushu, Japan. Ichthyol Res 61:393–398

  5. Matsuzaki SS, Kodama K, Terui A, Yakeshima H, Sato T, Tominaga O, Maeda H, Tada M, Washitani I, Yoshida T (2011) Changes in the strictly freshwater fish fauna around Lake Mikata, Fukui Prefecture, Japan, inferred from monitoring data and ecological trait information. Jpn J Conserv Ecol 16:205–212 (in Japanese)

  6. Ministry of the Environment (MOE), Japan (2013). Red List revised 4th edition: brackish and freshwater fishes. http://www.env.go.jp/press/files/jp/21437.pdf. Accessed 9 November 2017 (in Japanese)

  7. Onikura N, Nakajima J, Inui R, Mizutani H, Kobayakawa M, Fukuda S, Mukai T (2011) Evaluating the potential of invasion by alien freshwater fishes in northern Kyushu Island, Japan, using the Fish Invasiveness Scoring Kit. Ichthyol Res 58:382–387

  8. Sato M, Kawaguchi Y, Yamanaka H, Okunaka T, Nakajima J, Mitani Y, Shimatani Y, Mukai Y, Onikura N (2010) Predicting the spatial distribution of the invasive piscivorous chub (Opsariichthys uncirostris uncirostris) in the irrigation ditches of Kyushu, Japan: a tool for the risk management of biological invasions. Biol Invasions 12:3677–3686

  9. Turner CR, Barnes MA, Xu CCY, Jones SE, Jerde CL, Lodge DM (2014) Particle size distribution and optimal capture of aqueous macrobial eDNA. Methods Ecol Evol 5:676–684

  10. Watanabe K (2012) Faunal structure of Japanese freshwater fishes and its artificial disturbance. Environ Biol Fish 94:533–547

  11. Yamanaka H, Minamoto T, Matsuura J, Sakurai S, Tsuji S, Motozawa H, Hongo M, Sogo Y, Kakimi N, Teramura I, Sugita M, Baba M, Kondo A (2017) A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant. Limnol 18:233–241

Download references

Acknowledgements

We appreciate Dr. Jun Nakajima for his assistance in field work. This study was supported by JSPS KAKENHI (no. 16K00630).

Author information

Correspondence to Hiroki Yamanaka.

About this article

Verify currency and authenticity via CrossMark

Cite this article

Yamanaka, H., Takao, D., Maruyama, A. et al. Species-specific detection of the endangered piscivorous cyprinid fish Opsariichthys uncirostris uncirostris, three-lips, using environmental DNA analysis. Ecol Res 33, 1075–1078 (2018). https://doi.org/10.1007/s11284-018-1612-2

Download citation

Keywords

  • Distribution
  • Environmental DNA
  • Noninvasive detection
  • Opsariichthys uncirostris uncirostris
  • Specific primers