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Root size and soil environments determine root lifespan: evidence from an alpine meadow on the Tibetan Plateau

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Ecological Research

Abstract

We used a minirhizotron system to investigate the influence of three major factors—root morphology, root depth, and season of root emergence—on root survivorship and longevity in a Kobresia humilis meadow on the Tibetan Plateau during the growing season of 2009. Root longevity was assessed by survival analysis, Kaplan–Meier analysis, and Cox proportional hazards regression. Root longevity was correlated positively with root diameter. A 17.5 % decrease in the risk of mortality was associated with a 0.1-mm increase in diameter. Roots distributed in the top 10 cm of the soil had significantly shorter longevities than roots at greater depths, with a 48 % decrease of mortality risk for each 10-cm increase in soil depth from the surface to 40 cm. Of all the factors examined, the season of root emergence had the strongest effect on root lifespan. Roots that emerged in May and June had shorter longevity than roots that emerged later in the year, and roots that emerged in September and October were more likely to survive over winter. Our findings indicated that life-history traits of roots in K. humilis meadows are highly heterogeneous, and this heterogeneity should be considered when modeling the contribution of roots to carbon and nitrogen fluxes in this type of meadow ecosystem. Moreover, temporal, spatial, and compositional variations in root longevity must be considered.

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References

  • Allison PD (1995) Survival analysis using the SAS system: a practical guide. SAS Institute, Cary, NC

    Google Scholar 

  • Anderson LJ, Comas LH, Lasko AN, Eissenstat DM (2003) Multiple risk factors in root survivorship: a 4-year study in Concord grape. New Phytol 158:489–501

    Article  Google Scholar 

  • Arnone JA, Zaller JG, Spehn EM, Niklaus PA, Wells CE, Körner C (2000) Dynamics of root systems in native grasslands: effects of elevated atmospheric CO2. New Phytol 147:73–78

    Article  CAS  Google Scholar 

  • Baddeley JA, Watson CA (2005) Influences of root diameter, tree age, soil depth and season on fine root survivorship in Prunus avium. Plant Soil 276:15–22

    Article  CAS  Google Scholar 

  • Bates TR, Dunst RM, Joy P (2002) Seasonal dry matter, starch and nutrient distribution in Concord grapevine roots. HortScience 37:313–316

    Google Scholar 

  • Brassard BW, Chen HYH, Bergeron Y (2009) Influence of environmental variability on root dynamics in northern forests. Crit Rev Plant Sci 28:179–197

    Article  Google Scholar 

  • Bryla DR, Bouma TJ, Eissenstat DM (1997) Root respiration in citrus acclimates to temperature and slows during drought. Plant Cell Environ 20:1411–1420

    Article  Google Scholar 

  • Burton AJ, Pregitzer KS, Hendrick RL (2000) Relationships between fine root dynamics and nitrogen availability in Michigan northern hardwood forest. Oecologia 125:389–399

    Article  Google Scholar 

  • Cantor A (1997) Extending SAS survival analysis techniques for medical research. SAS Institute, Cary, NC

    Google Scholar 

  • Cao GM, Tang YH, Mo WH, Wang YS, Li YN, Zhao XQ (2004) Grazing intensity alters soil respiration in an alpine meadow on the Tibetan Plateau. Soil Biol Biochem 36:237–243

    Article  CAS  Google Scholar 

  • Cox DR (1972) Regression models and life tables. J R Stat Soc B 34:187–220

    Google Scholar 

  • Craine JM, Lee WG (2003) Covariation in leaf and root traits for native and non-native grasses along an altitudinal gradient in New Zealand. Oecologia 134:471–478

    PubMed  CAS  Google Scholar 

  • Eissenstat DM, Yanai R (1997) The ecology of root lifespan. Adv Ecol Res 27:1–60

    Article  Google Scholar 

  • Eissenstat DM, Wells CE, Yanai RD, Whitbeck JL (2000) Building roots in a changing environment: implications for root longevity. New Phytol 147:33–42

    Article  CAS  Google Scholar 

  • Espeleta JF, Eissenstat DM (1998) Responses of citrus fine roots to localized soil drying: a comparison of seedlings and adult fruiting trees. Tree Physiol 18:113–119

    Article  PubMed  Google Scholar 

  • Finér L, Laine J (1998) Root dynamics at drained peatland sites of different fertility in southern Finland. Plant Soil 201:27–36

    Article  Google Scholar 

  • Fitter AH, Self GK, Brown TK, Bogie DS, Graves JD, Benham D, Ineson P (1999) Root production and turnover in an upland grassland subjected to artificial soil warming respond to radiation flux and nutrients, not temperature. Oecologia 120:575–581

    Article  Google Scholar 

  • Forbes PJ, Black KE, Hooker JE (1997) Temperature-induced alteration to root longevity in Lolium perenne. Plant Soil 190:87–90

    Article  CAS  Google Scholar 

  • Fukuzawa K, Shibata H, Takigi K, Satoh F, Koike T, Sasa K (2007) Vertical distribution and seasonal pattern of fine-root dynamics in a cool-temperate forest in northern Japan: implication of the understory vegetation, Sasa dwarf bamboo. Ecol Res 22:485–495

    Article  Google Scholar 

  • Gill RA, Jackson RB (2000) Global patterns of root turnover for terrestrial ecosystems. New Phytol 147:13–31

    Article  Google Scholar 

  • Gill RA, Burke IC, Lauenroth WK, Milchunas DG (2002) Longevity and turnover of roots in the shortgrass steppe: influence of diameter and depth. Plant Ecol 159:241–251

    Article  Google Scholar 

  • Guo DL, Mitchell RJ, Hendricks JJ (2004) Fine root branch orders respond differentially to carbon source-sink manipulations in a longleaf pine forest. Oecologia 140:450–457

    Article  PubMed  Google Scholar 

  • Guo DL, Michell RJ, Withing JM, Fan PP, Hendricks JJ (2008) Endogenous and exogenous controls of root lifespan, mortality and nitrogen flux in a longleaf pine forest: root branch order predominates. J Ecol 96:737–745

    Article  CAS  Google Scholar 

  • Hendrick RL, Pregitzer KS (1992) The demography of fine roots in a northern hardwood forest. Ecology 73:1094–1104

    Article  Google Scholar 

  • Hendrick RL, Pregitzer KS (1993) Patterns of fine root mortality in two sugar maple forests. Nature 361:59–61

    Article  Google Scholar 

  • Hendrick RL, Pregitzer KS (1996) Applications of minirhizotrons to understand root function in forests and other natural ecosystems. Plant Soil 185:293–304

    Article  CAS  Google Scholar 

  • Hendrick RL, Pregitzer KS (1997) The relationship between fine root demography and the soil environment in northern hardwood forests. Ecoscience 4:99–105

    Google Scholar 

  • Huang B, Duncan RR, Carrow RN (1997) Drought-resistance mechanisms of seven warm-season turfgrasses under surface soil drying. II. Root aspects. Crop Sci 37:1863–1869

    Article  Google Scholar 

  • Jackson RB, Mooney HA, Schulze ED (1997) A global budget for fine root biomass, surface area, and mineral nutrients. Proc Natl Acad Sci USA 94:7362–7366

    Article  PubMed  CAS  Google Scholar 

  • Johnson MG, Phillips DL, Tingey DT, Storm MJ (2000) Effects of elevated CO2, N-fertilization, and season on survival of ponderosa pine fine roots. Can J For Res 30:220–228

    Google Scholar 

  • Joslin JD, Wolfe MH, Hanson PJ (2000) Effects of altered water regimes on forest root systems. New Phytol 147:117–129

    Article  Google Scholar 

  • Kaplan EL, Meier P (1958) Nonparametric estimation from incomplete observations. J Am Stat Assoc 5:457–481

    Article  Google Scholar 

  • Kern CC, Friend AL, Johnson JM-F, Coleman MD (2004) Fine root dynamics in a developing Populus deltoides plantation. Tree Physiol 24:651–660

    Article  PubMed  Google Scholar 

  • Kosola KR, Eissenstat DM (1994) The fate of surface roots of citrus seedlings in dry soil. J Exp Bot 45:1639–1645

    Article  CAS  Google Scholar 

  • Kosola KR, Eissenstat DM, Graham JD (1995) Root demography of mature citrus trees: the influence of Phytophthora nicotianae. Plant Soil 171:283–288

    Article  CAS  Google Scholar 

  • Krift VD, Berendse F (2002) Root life spans of four grass species from habitats differing in nutrient availability. Funct Ecol 16:198–203

    Article  Google Scholar 

  • Li WH, Zhou XM (1998) Ecosystems of Qinghai-Tibetan Plateau and approach for their sustainable management. Studies on the Qinghai-Tibetan Plateau Series. Guangdong Science and Technology, Guangzhou

    Google Scholar 

  • Li YN, Wang XQ, Gu S, Fu YL, Du MY, Zhao L, Zhao XQ, Yu GR (2004) Integrated monitoring of alpine vegetation types and its primary production. Acta Geogr Sin 59:40–48

    Google Scholar 

  • Majdi H, Andersson P (2005) Fine root production and turnover in a Norway spruce stand in northern Sweden: effects of nitrogen and water manipulation. Ecosystems 8:191–199

    Article  CAS  Google Scholar 

  • Nadelhoffer KJ (2000) Research review: the potential effects of nitrogen deposition on fine-root production in forest ecosystems. New Phytol 147:131–139

    Article  CAS  Google Scholar 

  • Nadelhoffer KJ, Aber JD, Melillo JM (1985) Fine roots, net primary production, and soil nitrogen availability: a new hypothesis. Ecology 66:1377–1390

    Article  Google Scholar 

  • Parton WJ, Greenland D (1987) Central plains experimental range. In: Greenland D (ed) The climates of the long-term ecological research sites. University of Colorado Press, Boulder, pp 23–29

    Google Scholar 

  • Peek MS, Leffler AJ, Ivans CY, Ryel RJ, Caldwell MM (2005) Fine root distribution and persistence under field conditions of three co-occurring Great Basin species of different life form. New Phytol 165:171–180

    Article  PubMed  Google Scholar 

  • Peek MS, Leffler AJ, Hipps L, Sasha I, Ryel RJ, Caldwell MM (2006) Root turnover and relocation in the soil profile in response to seasonal soil water variation in a natural stand of Utah juniper (Juniperus osteosperma). Tree Physiol 26:1469–1476

    Article  PubMed  Google Scholar 

  • Picon-Cochard C, Pilon R, Tarroux E, Pages L, Robertson J, Dawson L (2012) Effects of species, root branching order and season on the root traits of 13 perennial grass species. Plant Soil 353:47–57

    Article  CAS  Google Scholar 

  • Pregitzer KS (2002) Fine roots of trees: a new perspective. New Phytol 154:267–273

    Article  Google Scholar 

  • Pregitzer KS, Hendrick RL, Fogel R (1993) The demography of fine roots in response to patches of water and nitrogen. New Phytol 125:575–580

    Article  Google Scholar 

  • Pregitzer KS, King JS, Burton AJ, Brown SE (2000) Responses of tree fine roots to temperature. New Phytol 147:105–115

    Article  CAS  Google Scholar 

  • Pregitzer KS, de Forest JL, Burton AJ, Allen MF, Ruess RW, Hendrick RL (2002) Fine root architecture of nine North American trees. Ecol Monogr 72:293–309

    Article  Google Scholar 

  • Psarras G, Merwin IA, Lakso AN, Ray JA (2000) Root growth phonology, root longevity, and rhizosphere respiration of field grown ‘Mutsu’ apple trees on ‘Mailing 9’ rootstock. J Am Soc Hortic Sci 125:596–602

    Google Scholar 

  • Self GK, Brown TK, Grave J, Fitter AH (1995) Longevity and respiration rates of roots of upland grassland species in relation to temperature and atmospheric CO2 concentration (Abstract). J Exp Bot 46(suppl):25

    Google Scholar 

  • Steinaker DF, Wilson SD (2005) Belowground litter contributions to nitrogen cycling at a northern grassland-forest boundary. Ecology 86:2825–2833

    Article  Google Scholar 

  • Tingey DT, Phillips DL, Johnson MG (2000) Elevated CO2 and conifer roots: effects on growth, life span and turnover. New Phytol 147:87–103

    Article  CAS  Google Scholar 

  • Vogt KA, Crier CC, Vogt DJ (1986) Production, turnover and nutrient dynamics of above-and belowground detritus of world forests. Adv Ecol Res 15:303–377

    Article  Google Scholar 

  • Vogt KA, Vogt DJ, Palmiotto PA, Boon P, O’Hara J, Asbjornsen H (1996) Review of root dynamics in forest ecosystems grouped by climate, climatic forest type and species. Plant Soil 187:159–219

    Article  CAS  Google Scholar 

  • Wells CE, Eissenstat DM (2001) Marked differences in survivorship among apple roots of different diameters. Ecology 82:882–892

    Article  Google Scholar 

  • Wells CE, Glenn DM, Eissenstat DM (2002) Changes in the risk of fine root mortality with age: a case study in peach, Prunus persica (Rosaceae). Am J Bot 89:79–87

    Article  PubMed  Google Scholar 

  • West JB, Espeleta JF, Donovan LA (2004) Fine root production and turnover across a complex edaphic gradient of a Pinus palustrisAristida stricta savanna ecosystem. For Ecol Manag 19:397–406

    Article  Google Scholar 

  • Wu YB, Wu J, Deng YC, Tan HC, Du YG, Gu S, Tang YH, Cui XY (2011) Comprehensive assessments of root biomass and production in a Kobresia humilis meadow on the Qinghai-Tibetan Plateau. Plant Soil 338:497–510

    Article  CAS  Google Scholar 

  • Yi X, Yang Y (2006) A stable carbon isotopic approach for understanding the CO2 flux at the Haibei Alpine Meadow Ecosystem: a simple model. Ecol Mod 193:796–800

    Article  Google Scholar 

  • Yuan ZY, Chen HYH (2010) Fine root biomass, production, turnover rates, and nutrient contents in boreal forest ecosystems in relation to species, climate, fertility and stand age: literature review and meta-analyses. Crit Rev Plant Sci 29:204–221

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The research was supported by grants from the Natural Science Foundation of China (Nos. 41071174 and 31200367), Zhejiang Province (No. LQ12C03001), the Scientific Research Fund of Zhejiang Provincial Education Department (No. Y201121042), and the K. C. Wong Magna Fund of Ningbo University, for which the authors are grateful. We also thank the staff at the Haibei Alpine Meadow Ecosystem Research Station for their help and hospitality.

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Correspondence to Xiaoyong Cui.

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Wu, Y., Deng, Y., Zhang, J. et al. Root size and soil environments determine root lifespan: evidence from an alpine meadow on the Tibetan Plateau. Ecol Res 28, 493–501 (2013). https://doi.org/10.1007/s11284-013-1038-9

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  • DOI: https://doi.org/10.1007/s11284-013-1038-9

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