Abstract
Biomass from agriculture, forestry, and urban wastes is a potential renewable organic resource for energy generation. Many investigations have demonstrated that anaerobic fungi and methanogens could be co-cultured to degrade lignocellulose for methane generation. Thus, this study aimed to evaluate the effect of natural anaerobic fungi-methanogens co-culture on the methane production and lignocellulosic degradation of wastes from rice, corn and sugarcane. Hu sheep rumen digesta was used to develop a natural anaerobic fungi-methanogen co-culture. The substrates were rice straw (RS), rich husk (RH), corn stover (CS), corn cobs (CC), and sugarcane baggage (SB). Production of total gas and methane, metabolization rate of reducing sugar, glucose, and xylose, digestibility of hemicellulose and cellulose, activity of carboxymethylcellulase and xylanase, and concentrations of total acid and acetate were highest (P < 0.05) in CC, moderate (P < 0.05) in RS and CS, and lowest (P < 0.05) in SB and RH. The pH, lactate and ethanol were lowest (P < 0.05) in CC, moderate (P < 0.05) in RS and CS, and lowest (P < 0.05) SB and RH. Formate was lowest (P < 0.05) in CC, RS and CS, moderate (P < 0.05) in SB, and lowest (P < 0.05) in RH. Therefore, this study indicated that the potential of methane production and lignocellulosic degradation by natural anaerobic fungi-methanogens co-culture were highest in CC, moderate in RS and CS, and lowest in SB and RH.
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The data used in the current research are available upon reasonable request from the corresponding author.
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Acknowledgements
We would like to thank Zhenxiang Meng and Yihan Xue for their help when conducting experiments.
Funding
This work was supported by the National Natural Science Foundation of China (Grant Numbers; 3237200386; 32061143034).
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Conceptualization, Writing – original draft: Y.Y.K. and M.A.; Methodology: Y.YK., M.A., Y.X. and Y.L.; investigation: Y.Y.K., M.A., Y.X., Y.Z., Y.L. and Z.S.; formal analysis: Y.Y.K., M.A., Y.X., Y.Z., Y.L. and Z.S.; data curation: Y.Y.K., M.A. and Z.S.; project administration: Y.C.; funding acquisition: W.Z. and Y.C.; Conceptualization, Project administration, Supervision, Writing – review and editing: Y.C. All authors reviewed the manuscript.
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Kyawt, Y.Y., Aung, M., Xu, Y. et al. Methane production and lignocellulosic degradation of wastes from rice, corn and sugarcane by natural anaerobic fungi-methanogens co-culture. World J Microbiol Biotechnol 40, 109 (2024). https://doi.org/10.1007/s11274-024-03938-8
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DOI: https://doi.org/10.1007/s11274-024-03938-8