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Inhibitory effects of hinokitiol on the development and pathogenicity of Colletotrichum gloeosporioides

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Abstract

Postharvest anthracnose of mango fruit caused by Colletotrichum gloeosporioides is a devastating fungal disease, which causes tremendous quality deterioration and economic losses. Hinokitiol, an environmentally friendly natural compound, is effective in controlling a variety of postharvest fungal diseases. However, there is still a lack of research on the inhibitory effect of hinokitiol on C. gloeosporioides and its possible modes of action. In the present study, the activity of hinokitiol against C. gloeosporioides and its potential mechanisms involved have been investigated. We found that hinokitiol treatment could effectively inhibit the virulence of C. gloeosporioides to harvested mango fruit. After treatment with 8 mg/L hinokitiol, the mycelial growth of C. gloeosporioides was completely inhibited. When the concentration of hinokitiol reached 9 mg/L, the spore germination rate of C. gloeosporioides decreased to 2.43% after 9 h of cultivation. The inhibitory effect is mainly due to the attenuation in cell viability, and impairment in plasma membrane followed by leakage of cytoplasmic contents such as nucleic acids, proteins, and soluble carbohydrates, which ultimately leads to the destruction of cell structure. Furthermore, hinokitiol suppressed the expression of pathogenicity-related genes, leading to reduced infection activity. Collectively, these results suggest that hinokitiol may be an excellent bio-fungicides for the management of mango anthracnose.

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Acknowledgements

This work was supported by the grant from National Natural Science Foundation of China (32202565), Fujian Provincial Natural Science Foundation (2022J05151), and Initial Research Fund of Jimei University (ZQ2022011).

Funding

This research was funded by the grant from National Natural Science Foundation of China (32202565), Fujian Provincial Natural Science Foundation (2022J05151), and Initial Research Fund of Jimei University (ZQ2022011).

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  1. Shen Zhang and Jingyi Wang contributed equally to this work.

Authors and Affiliations

  1. College of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China

    Shen Zhang, Jingyi Wang, Huimin Sun, Jing Yang, Jiajia Zhao & Ying Wang

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  1. Shen Zhang
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  3. Huimin Sun
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Contributions

Conceptualization: Shen Zhang, Ying Wang; Formal analysis: Jingyi Wang, Shen Zhang; Investigation and Data curation: Huimin Sun, Jing Yang, Jiajia Zhao; Methodology: Ying Wang; Supervision: Shen Zhang, Ying Wang; Writing-original draft: Shen Zhang, Huimin Sun; Writing-review and editing: Shen Zhang, Jingyi Wang, Huimin Sun, Ying Wang. Funding acquisition: Ying Wang. All authors reviewed the manuscript.

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Correspondence to Ying Wang.

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Zhang, S., Wang, J., Sun, H. et al. Inhibitory effects of hinokitiol on the development and pathogenicity of Colletotrichum gloeosporioides. World J Microbiol Biotechnol 39, 356 (2023). https://doi.org/10.1007/s11274-023-03810-1

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