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Cell free extract-mediated biogenic synthesis of ZnONPs and their application with kanamycin as a bactericidal combination

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Abstract

Antimicrobial resistance (AMR) is a main public health issue and a challenge for the scientific community all over the globe. Hence, there is a burning need to build new bactericides that resist the AMR. The ZnONPs were produced by cell free extract of mint (Mentha piperita L.) leaves. Antibiotics that are ineffective against resistant bacteria like Escherichia coli and Staphylococcus aureus were treated. The antibiotics were first screened, and then antibacterial activity was checked by disk diffusion, and MIC of Mp-ZnONPs individually and using Kanamycin (KAN) were determined against these pathogens by broth microdilution method. The synergism between Mp-ZnONPs and KAN was confirmed by checkerboard assay. The MIC showed robust antibacterial activity against the tested pathogens. The combination of KAN and Mp-ZnONPs reduces the MIC of KAN as it efficiently inhibits E. coli’s growth, and KAN significantly enhances the antibacterial activity of Mp-ZnONPs. Taken together, Mp-ZnONPs have strong antimicrobial activity, and KAN significantly improves it against the tested pathogens, which would offer an effective, novel, and benign therapeutic methodology to regulate the incidence. The combination of Mp-ZnONPs and KAN would lead to the development of novel bactericides, that could be used in the formulation of pharmaceutical products.

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Acknowledgements

SSS and TMM are thankful to the Ministry of Science and Higher Education of the Russian Federation within the framework of the state task in the field of scientific activity (No. 0852-2020-0029) for the financial support.

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There was no external funding received for this research work.

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Authors and Affiliations

  1. Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, MS, 444 602, India

    Asma Saleha, Sudhir S. Shende, Pramod Ingle, Mahendra Rai & Aniket Gade

  2. Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, 344090, Russia

    Sudhir S. Shende & Tatiana M. Minkina

  3. Department of Microbiology, Nicolaus Copernicus University, 87-100, Torun, Poland

    Mahendra Rai & Aniket Gade

  4. Department of Biological Sciences and Biotechnology, Institute of Chemical Technology, Mumbai, Maharashtra, 400019, India

    Aniket Gade

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  1. Asma Saleha
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  4. Mahendra Rai
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Contributions

AS, SSS, MR, and AG conceived and designed the research. AS, SSS, and PI conducted experiments. SSS, PI, and AG contributed new reagents, analysis, and characterization of NPs. AS, SSS, AG, and MR analyzed data. AS and SSS wrote the original manuscript. MR and AG supervised the work. AS, SSS, TMM, MR, and AG revised and edited the manuscript. All authors read and approved the manuscript.

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Correspondence to Sudhir S. Shende or Aniket Gade.

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Saleha, A., Shende, S.S., Ingle, P. et al. Cell free extract-mediated biogenic synthesis of ZnONPs and their application with kanamycin as a bactericidal combination. World J Microbiol Biotechnol 39, 334 (2023). https://doi.org/10.1007/s11274-023-03777-z

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