Abstract
Intestinal mucositis is a commonly reported side effect in oncology patients undergoing chemotherapy and radiotherapy. Probiotics, prebiotics, and synbiotics have been investigated as alternative therapeutic approaches against intestinal mucositis due to their well-known anti-inflammatory properties and health benefits to the host. Previous studies showed that the potential probiotic Lactobacillus delbrueckii CIDCA 133 and the prebiotic Fructooligosaccharides (FOS) alleviated the 5-Fluorouracil (5-FU) chemotherapy-induced intestinal mucosa damage. Based on these previous beneficial effects, this work evaluated the anti-inflammatory property of the synbiotic formulation containing L. delbrueckii CIDCA 133 and FOS in mice intestinal mucosa inflammation induced by 5-FU. This work showed that the synbiotic formulation was able to modulate inflammatory parameters, including reduction of cellular inflammatory infiltration, gene expression downregulation of Tlr2, Nfkb1, and Tnf, and upregulation of the immunoregulatory Il10 cytokine, thus protecting the intestinal mucosa from epithelial damage caused by the 5-FU. The synbiotic also improved the epithelial barrier function by upregulating mRNA transcript levels of the short chain fatty acid (SCFA)-associated GPR43 receptor and the occludin tight junction protein, with the subsequent reduction of paracellular intestinal permeability. The data obtained showed that this synbiotic formulation could be a promising adjuvant treatment to be explored against inflammatory damage caused by 5-FU chemotherapy.
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The current study’s data are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors would like to acknowledge the Pró-Reitoria de Pesquisa - Universidade Federal de Minas Gerais, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) for their financial support and fellowships. We would also like to acknowledge the CIDCA center (Center for Research and Development in Food Cryotechnology) and Pablo F. Pérez of the National University of La Plata, Argentine, for bacterial strain supply.
Funding
This research was funded by Fundação de Amparo à Pesquisa do Estado de Minas Gerais -FAPEMIG (grant numbers: RED-00132-16 and APQ-00593-14), and Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq) (grant number: 312045/2020-4).
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Conceptualization: PM-A, LCLJ, MMD, and VA; Methodology: FALB, VLB, GMC, GAB, TFS, NDC-R, KDV, ÊF; Formal analysis and investigation: LMT, LCLJ; Writing-original draft preparation: LMT, MFA, ASF, GMC, LCLJ; Writing-review and editing: JGL, SOAF, VNC, MMD, PM-A, AB, EF, FSM, and VA; Supervision: VA; Funding acquisition: VA. All authors read and approved the final manuscript.
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Tavares, L.M., de Jesus, L.C.L., Batista, V.L. et al. Synergistic synbiotic containing fructooligosaccharides and Lactobacillus delbrueckii CIDCA 133 alleviates chemotherapy-induced intestinal mucositis in mice. World J Microbiol Biotechnol 39, 235 (2023). https://doi.org/10.1007/s11274-023-03679-0
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DOI: https://doi.org/10.1007/s11274-023-03679-0