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Nutrient removal and biogas upgrade using co-cultivation of Chlorella vulgaris and three different bacteria under various GR24 concentrations by induction with 5-deoxystrigol

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Abstract

Algae symbiosis technology shows great potential in the synchronous treatment of biogas slurry and biogas, which has promising applications. For improving nutrients and CO2 removal rates, the present work constructed four microalgal systems: Chlorella vulgaris (C. vulgaris) monoculture, C. vulgaris—Bacillus licheniformis (B. licheniformis), C. vulgaris—activated sludge, and C. vulgaris—endophytic bacteria (S395-2) to simultaneously treat biogas as well as biogas slurry under GR24 and 5DS induction. Our results showed that the C. vulgaris—endophytic bacteria (S395-2) showed optimal growth performance along with photosynthetic activity under the introduction of GR24 (10−9 M). Under optimal conditions, CO2 removal efficiency form biogas, together with chemical oxygen demand, total phosphorus and total nitrogen removal efficiencies from biogas slurry reached 67.25 ± 6.71%, 81.75 ± 7.93%, 83.19 ± 8.32%, and 85.17 ± 8.26%, respectively. The addition of symbiotic bacteria isolated from microalgae can promote the growth of C. vulgaris, and the exogenous addition of GR24 and 5DS can strengthen the purification performance of the algae symbiosis to achieve the maximum removal of conventional pollutants and CO2.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This research was supported by ABA Chemicals for funding support and the National Major Science and Technology Project (2017ZX07204-005).

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Author notes

  1. Lixing Shu and Junfeng Li: Co-first authors

Authors and Affiliations

  1. Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, People’s Republic of China

    Lixing Shu & Zheng Zheng

  2. School of Advanced Materials and Engineering, Jiaxing Nanhu University, Jiaxing, 314001, People’s Republic of China

    Junfeng Li & Jun Xu

  3. Jiaxing Key Laboratory of Preparation and Application of Advanced Materials for Energy Conservation and Emission Reduction, Jiaxing, 314001, People’s Republic of China

    Junfeng Li & Jun Xu

Authors
  1. Lixing Shu
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  2. Junfeng Li
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  3. Jun Xu
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  4. Zheng Zheng
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Contributions

LS was in charge of investigation, drafting, reviewing and editing. JL was responsible for methodology, data management and interpretation. JX contributed to acquiring funding and analyzing formally. ZZ: contributed to acquiring funding and study conception.

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Correspondence to Zheng Zheng.

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Shu, L., Li, J., Xu, J. et al. Nutrient removal and biogas upgrade using co-cultivation of Chlorella vulgaris and three different bacteria under various GR24 concentrations by induction with 5-deoxystrigol. World J Microbiol Biotechnol 39, 245 (2023). https://doi.org/10.1007/s11274-023-03647-8

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