Abstract
Benzopyrene is a high-molecular-weight polycyclic aromatic hydrocarbon that is highly recalcitrant and induces carcinogenic effects. CsrA is a conserved regulatory protein that controls the translation and stability of its target transcripts, having negative or positive effects depending on the target mRNAs. It is known that Bacillus licheniformis M2-7 has the ability to grow and survive in certain concentrations of hydrocarbons such as benzopyrene, prompted in part by CsrA, as is present in gasoline. However, there are a few studies that reveal the genes involved in that process. To identify the genes involved in the Bacillus licheniformis M2-7 degradation pathway, the plasmid pCAT-sp containing a mutation in the catE gene was constructed and used to transform B. licheniformis M2-7 and generate a CAT1 strain. We determined the capacity of the mutant B. licheniformis (CAT1) to grow in the presence of glucose or benzopyrene as a carbon source. We observed that the CAT1 strain presented increased growth in the presence of glucose but a statistically considerable decrease in the presence of benzopyrene compared with the wild-type parental strain. Additionally, we demonstrated that the Csr system positively regulates its expression since it was observed that the expression of the gene in the mutant strain LYA12 (M2-7 csrA:: Sp, SpR) was considerably lower than that in the wild-type strain. We were thus able to propose a putative regulation model for catE gene in B. licheniformis M2-7 strain by CsrA regulator in the presence of benzopyrene.
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Acknowledgements
This work was supported by grant 18-PFA-IIDTTT-000089-L000-DF from SAGARPA. Giselle Yamilet Morales Blancas and José Daniel Reyna Terán thank CONACyT for M.Sc. scholarships.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [Alejandro Bolaños Dircio], [José Alberto Hernandez Eligio], [Erubiel Toledo Hernández], [Augusto Rojas Aparicio] and [Carlos Ortuño Pineda]. The first draft of the manuscript was written by [Yanet Romero Ramírez], [Jeiry Toribio Jiménez] and [Miguel Ángel Rodríguez Barrera] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Morales-Blancas, G.Y., Reyna-Terán, J.D., Hernández-Eligio, J.A. et al. The catE gene of Bacillus licheniformis M2-7 is essential for growth in benzopyrene, and its expression is regulated by the Csr system. World J Microbiol Biotechnol 39, 177 (2023). https://doi.org/10.1007/s11274-023-03630-3
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DOI: https://doi.org/10.1007/s11274-023-03630-3