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Evaluation of antimicrobial activity of Streptomyces pactum isolated from paddy soils and identification of bioactive volatile compounds by GC-MS analysis

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Abstract

The objective of this study was to isolate Streptomyces sp. from north paddy soils of Iran and investigation and identification of the bioactive compounds by carrying out GC-MS analysis. Antimicrobial activity was investigated by well diffusion agar technique against the pathogenic microorganisms including Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 4698, Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 9144, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 43071. Based on the results of gene sequencing of gene 16S rRNA and phylogenetic analysis, the isolated sample belongs to the genus Streptomyces with the highest degree of resemblance (99/87%) to the Streptomyces pactum strain ACT12. The isolate showed a broad spectrum of antibacterial activity against test microorganisms. This isolate showed maximum antibacterial activity against M. luteus (25 ± 0.5 mm) and the most resistant microorganism against antibacterial activity of this isolate was P. aeruginosa (9 ± 0.5 mm). The contact bioautography method was used to detect compounds that were responsible for antimicrobial activity and showed the active compounds with Rf values of 0.8–0.9. The identification of bioactive metabolites were performed using gas chromatography-mass spectrometry (GC-MS). GC-MS analysis of the extract showed the presence of 15 volatile compounds. The main compounds were Methyl-3-(3,5-ditertbutyl-4-hydroxyphenyl) (10.88%) and Dibutyl phthalate (8.34%) in comparison with other bioactive compounds. The results showed that north paddy soils of Iran are a rich source of microbial flora for the production of antimicrobial compounds and useful for antimicrobial compounds discovery from Streptomyces sp.

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Data availability

The data in this study are available from the corresponding author on reasonable request.

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Acknowledgements

The support from the microbiology research laboratory in Islamic Azad University, Lahijan Branch, is greatly acknowledged.

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The authors declare that no funds and grants were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran

    Seyedeh Zahra Mirsonbol, Khosro Issazadeh & Mirsasan Mirpour

  2. Department of Chemistry, Lahijan Branch, Islamic Azad University, Lahijan, Iran

    Saeid Zarrabi

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  1. Seyedeh Zahra Mirsonbol
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  2. Khosro Issazadeh
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  3. Saeid Zarrabi
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  4. Mirsasan Mirpour
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Contributions

S.Z.M. performed the experiments. Kh.I. and S.Z. analayzed all data. S.Z.M. wrote the paper. Kh. I. and S.Z. and M.M. revised the manuscript. All authors contributed to the study conception and design. All authors read and approved the final manuscript.

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Correspondence to Khosro Issazadeh.

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Mirsonbol, S.Z., Issazadeh, K., Zarrabi, S. et al. Evaluation of antimicrobial activity of Streptomyces pactum isolated from paddy soils and identification of bioactive volatile compounds by GC-MS analysis. World J Microbiol Biotechnol 39, 63 (2023). https://doi.org/10.1007/s11274-022-03508-w

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