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8-Hydroxyquinoline a natural chelating agent from Streptomyces spp. inhibits A549 lung cancer cell lines via BCL2/STAT3 regulating pathways

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Abstract

Biomolecules from Streptomyces spp. are emerging sources of natural drugs and have been focused on over the decade. The discovery of bioactive chemotherapeutic molecules from soil Streptomyces spp. has opened the medium for the search for natural drugs. In the current study, 8-HOQ was extracted and purified from soil Streptomyces spp. and was evaluated on A549 and BEAS cell lines. The apoptotic and caspase mediated pathways were evaluated using cell proliferation, dual fluorescent staining, migration, invasion and mRNA as well as protein quantification of apoptotic markers. In vitro cytotoxicity test revealed that 8-HOQ possesses potent cytotoxicity activities with IC50 values of 26 µM, 5 µM, 7.2 µM at 24 h, 48 h, and 72 h respectively against A549 lung cancer cell lines. The result also demonstrated that 8-HOQ from Streptomyces spp significantly inhibited the A549 lung cancer cell lines and activated the intrinsic pathways of apoptosis. The caspase-3 and caspase-8 activities were potentially elevated in 8-HOQ treated A549 cell lines and confirmed that 8-HOQ mediated A549 cancer cell death through the intrinsic pathway. The results explored caspase-mediated apoptosis as a mechanism underlying the inhibition of cancer cell viability in a dose-dependent manner. The expression of P53, BCL2 and STAT3 were inhibited in A549 cell lines and confirmed the metastasis inhibitory potential of 8-HOQ by blocking migration and invasion in A549 cell lines. These results indicated that 8-HOQ from Streptomyces spp. potentially inhibited growth and migration of A549 lung cancer cell lines.

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Authors and Affiliations

  1. Division of Microbiology and Biotechnology, Entomology Research Institute, Loyola College, Chennai, Tamil Nadu, 600 034, India

    Joseph Devadass Balthazar & Maria Packiam Soosaimanickam

  2. PG and Research Department of Advanced Zoology and Biotechnology, Loyola College, Chennai, Tamil Nadu, 600 034, India

    Maria Packiam Soosaimanickam

  3. Gleneagles Global Health City, Perumbakkam, Chennai, Tamil Nadu, 600 100, India

    C. Emmanuel

  4. Pondicherry Centre for Biological Science and Educational Trust, Sundararaja Nagar, Pondicherry, 605004, India

    Thirugnansambantham Krishnaraj & Hairul-Islam Mohamed Ibrahim

  5. Camel Research Center, King Faisal University, P.O. Box 400, AlHufuf, 31982, Al-Ahsa, Saudi Arabia

    Abdullah Sheikh

  6. Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai, India

    Thirugnansambantham Krishnaraj

  7. Molecular Department, Al-Qassim Regional Laboratory, Ministry of Health, Buraydah, 52385, Al-Qassim, Saudi Arabia

    Saleh Fahad Alghafis

  8. Biological Sciences Department, College of Science, King Faisal University, Hofouf, 31982, Al-Hasa, Saudi Arabia

    Hairul-Islam Mohamed Ibrahim

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  1. Joseph Devadass Balthazar
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  2. Maria Packiam Soosaimanickam
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  3. C. Emmanuel
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  4. Thirugnansambantham Krishnaraj
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  5. Abdullah Sheikh
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  6. Saleh Fahad Alghafis
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  7. Hairul-Islam Mohamed Ibrahim
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Contributions

JB: Experimental design, troubleshoot, perform molecule extraction, cell culture experiments, data curation, initial draft manuscript preparation, editing, final corrections. MP: Data curation, final draft manuscript preparation, editing, final corrections. CM: Data curation, final draft manuscript preparation, editing, final corrections. HI: Experimental design, troubleshoot, perform cell culture experiments, data curation, draft manuscript preparation, editing. KT: Perform fluorescent experiments, molecular docking, initial draft preparation and corrections. AS: mRNA and protein experiments, final draft preparation and corrections. SF: Final draft corrections, statistics, data curation and final editing.

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Correspondence to Joseph Devadass Balthazar.

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Balthazar, J.D., Soosaimanickam, M.P., Emmanuel, C. et al. 8-Hydroxyquinoline a natural chelating agent from Streptomyces spp. inhibits A549 lung cancer cell lines via BCL2/STAT3 regulating pathways. World J Microbiol Biotechnol 38, 182 (2022). https://doi.org/10.1007/s11274-022-03368-4

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