Abstract
Bacterial intercellular communication mediated by small diffusible molecules, known as quorum sensing (QS), is a common mechanism for regulating bacterial colonisation strategies and survival. Influence on QS by plant-derived molecules is proposed as a strategy for combating phytopathogens by modulating their virulence. This work builds upon other studies that have revealed plant-derived QS inhibitors extracted from oak bark (Quercus sp.). It was found that co-incubation of Pectobacterium carotovorum VKM-B-1247 with oak bark extract (OBE) reduced the production of acyl-HSL. This was accompanied by a dose-dependent decrease in the bacterial cellulolytic and protease activity. At the transcriptomic level, the OBE treatment suppressed the main QS-related genes expR/expI. Potato tubers pre-treated with OBE showed resistance to a manifestation of soft-rot symptoms. Analysis of the component composition of the OBE identified several biologically active molecules, such as n-hexadecanoic acid, 2,6-di-tert-butyl-4-methylphenol, butylated hydroxytoluene (BHT), gamma-sitosterol, lupeol, and others. Molecular docking of the binding energy between identified molecules and homology models of LuxR–LuxI type proteins allow to identify potential inhibitors. Collectively, obtained results figure out great potential of widely distributed oak-derived plant material for bacterial control during storage of potato.
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Acknowledgements
Bioluminescence E.coli MG 1655 strain was kindly provided by Prof. Ilya V. Manukhov (GosNII Genetica, Moscow). Chromobacterium violaceum strains were kindly provided by Prof. Dmitry G. Deryabin (Federal Scientific Center for Biological Systems and Agricultural Technologies, RAS, Orenburg). This work was partially performed using resources of the Research Resource Center & Natural Resource Management and Physico-Chemical Research (University of Tyumen).
Funding
This work was supported by the Russian Science Foundation (Grant No. 19-76-30005) in sections devoted to molecular-genetic manipulations. The work was performed within the state assignment of the Ministry of Science and Higher Education of the Russian Federation for 2020–2024 (No. FEWZ-2020-0006)
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ASV conceived and designed the study. DVP, AVI, RS, AVV, EAR performed the experiments. ASV, DVP, RS, AVV, EAR analyzed and interpreted the data. ASV, EAR drafted and wrote the manuscript. ASV supervised the study. All authors approved the final version of the manuscript.
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Vasilchenko, A.S., Poshvina, D.V., Sidorov, R.Y. et al. Oak bark (Quercus sp. cortex) protects plants through the inhibition of quorum sensing mediated virulence of Pectobacterium carotovorum. World J Microbiol Biotechnol 38, 184 (2022). https://doi.org/10.1007/s11274-022-03366-6
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DOI: https://doi.org/10.1007/s11274-022-03366-6