Abstract
Sanguinarine and chelerytrine have antibacterial and anti-inflammatory effects and is the main active ingredients of growth promoters in animals. Currently, Sanguinarine and chelerytrine were extracted from the capsules of the medicinal plant Macleaya cordata. However, the biomass of M. cordata nonmedicinal parts (leaves) accounted for a large proportion and contained a rich presentation of protopine and allocryptopine which are the precursor compounds of sanguinarine and chelerytrine. The aim of this study was to develop a new method for producing sanguinarine and chelerytrine through yeast transformation of protopine and allocryptopine in M. cordata leaves. First, we isolated different genes from Papaver somniferum (PsP6H, PsCPR, PsDBOX), Eschscholtzia californica (EcP6H), Cucumis sativus (CuCPR), Arabidopsis thaliana (AtCPR) and M. cordata (Mc11229, Mc11218, Mc6408, Mc6407, Mc19967, Mc13802). Additionally, some of the gene sequences were codon optimized. Then, we transformed these genes into yeast cells to compare the catalytic efficiency. Second, we used the most efficient strains to biotransform the leaves of M. cordata. Finally, we obtained 85.415 ± 11.887 ng mL−1 sanguinarine and 4.288 ± 1.395 ng mL−1 chelerytrine, which was more than 2–3 times the content in leaves of M. cordata. Overall, we using the nonmedicinal parts of M. cordata and successfully obtained sanguinarine and chelerytrine by the plant–microbial hybrid synthesis method.
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Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- SAN:
-
Sanguinarine
- PRO:
-
Protopine
- ALL:
-
Allocryptopine
- CHE:
-
Chelerythrine
- P6H:
-
Protopine 6-hydroxylase
- CPR:
-
Cytochrome P450 reductase
- DBOX:
-
Dihydrobenzophenanthridine oxidase
- PMHS:
-
Plant–microbial hybrid synthesis
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Funding
This work was supported by the “Hunan Provincial Natural Science Foundation of China (Grant Nos. 2021JJ20032, 2020JJ4353)”; “Hunan Provincial Key Research and Development Project (Grant No. 2020NK2031)”; “The science and technology innovation Program of Hunan Province (Grant No. 2021RC3091)”; “Hunan Innovative Province Construction Special Fund Grant, Yuelu Mountain Seed Industry Innovation Project (Grant No. 2021NK1012)”.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by MS, LZ, PH and ZX. The first draft of the manuscript was written by MS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Sun, M., Zhong, X., Zhou, L. et al. Plant–microbe hybrid synthesis provides new insights for the efficient use of Macleaya cordata. World J Microbiol Biotechnol 38, 110 (2022). https://doi.org/10.1007/s11274-022-03295-4
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DOI: https://doi.org/10.1007/s11274-022-03295-4