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Genome analysis of Pseudomonas sp. 14A reveals metabolic capabilities to support epiphytic behavior

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Abstract

The surface of aboveground plant parts, known as the phyllosphere, is a habitat for various microorganisms called epiphytes establishing biotrophic interactions with their hosts. However, these communities can be affected by environmental and anthropogenic variations such as the application of agrochemicals. Thus, epiphytes have the capacity to survive in such environments. In this study, we obtained the genome of Pseudomonas sp. 14A, an epiphyte isolated from the pepper phyllosphere. The phylogenomic analyses suggested that Pseudomonas sp. 14A may be novel species closely related to P. moraviensis R28-S. Notably, the metabolic pathways proposed consistent with epiphytic lifestyle in Pseudomonas sp. 14A, were shared with other species displaying a different degree of phylogenetic relatedness. Furthermore, variations in configuration of metabolic gene clusters were observed, that could expand microbial metabolic diversity in close relatedness species, highlighting the relevance of microbial diversity associated with plants.

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Data availability

This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAGRQJ000000000. The version described in this paper is version JAGRQJ010000000.

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Acknowledgements

To Consejo Nacional de Ciencia y Tecnología-Secretaría de Educación Pública (CONACyT-SEP Project 236066). We thank the anonymous reviewers for their valuable comments on our manuscript.

Funding

This work was funded by Consejo Nacional de Ciencia y Tecnología-Secretaría de Educación Pública (CONACyT-SEP Project 236066).

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Authors and Affiliations

  1. Facultad de Agronomía y Veterinaria, Universidad Autónoma de San Luis Potosí, Km. 14.5 Carretera San Luis Potosí-Matehuala, Ejido Palma de la Cruz, Apartado Postal 32, CP 78321, Soledad de Graciano Sánchez, SLP, México

    Saúl Alejandro Medina-Salazar, Delia Xochil Vega-Manriquez, Ramón Jarquin-Gálvez, Gisela Aguilar-Benítez & José Pablo Lara-Ávila

  2. Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México

    Fernanda Cornejo-Granados, Edgar Equihua-Medina & Adrian Ochoa-Leyva

  3. CONACYT, Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México

    Moisés Roberto Vallejo-Pérez

  4. Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Tlaxcala, Mexico

    Rigoberto Castro-Rivera

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  1. Saúl Alejandro Medina-Salazar
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  2. Fernanda Cornejo-Granados
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  3. Edgar Equihua-Medina
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  10. José Pablo Lara-Ávila
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Contributions

JPLA conceived and designed study. SAMS, JPLA conducted in silico experiments. SAMS, DXVM, GAB, RJG, MRVP, JPLA analyzed and interpreted data. FCG, EEM, RCR, AOL contributed analytical tools. SAMS, JPLA wrote the paper. All authors read and approved the manuscript.

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Correspondence to José Pablo Lara-Ávila.

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Medina-Salazar, S.A., Cornejo-Granados, F., Equihua-Medina, E. et al. Genome analysis of Pseudomonas sp. 14A reveals metabolic capabilities to support epiphytic behavior. World J Microbiol Biotechnol 38, 49 (2022). https://doi.org/10.1007/s11274-022-03238-z

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