Abstract
Helicobacter pylori infection is an essential factor in the development of human gastric diseases, but its pathogenic mechanism is still unclear. In this work we have showed that, the LC3II levels were increased and β1-integrin levels were decreased in H. pylori-positive human gastric tissue samples and H. pylori co-cultured GES-1 cells. There was significant upregulation of LC3II levels and downregulation of P62 levels in GES-1 cells after β1-integrin knockdown co-cultured with H. pylori. This indicated that β1-integrin downregulation promoted autophagy in GES-1 cells after H. pylori infection. The cell apoptosis rate and poly ADP-ribose polymerase (PARP) and caspase-3 activities were increased in GES-1 cells pretreated with 3-methyladenine (3-MA ) after H. pylori infection. Furthermore, there was a significant decrease in apoptosis of β1-integrin knockdown GES-1 cells co-cultured with H. pylori; apoptosis was also downregulated in β1-integrin knockdown- and 3-MA-treated GES-1 cells co-cultured with H. pylori. Correspondingly, PARP and caspase-3 activities were decreased in β1-integrin knockdown cells co-cultured with H. pylori and β1-integrin knockdown-3-MA-treated-1 cells with H. pylori infection. Thus, β1-integrin is a novel autophagy and apoptosis regulator during H. pylori infection. However, inhibition of autophagy did not reverse the decrease in apoptosis caused by downregulation of β1-integrin.
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Acknowledgements
All authors thank the Medical Research Center of Binzhou Medical University for providing large-scale instrument use platforms, and the Department of Pathogen Biology, School of Basic Medical Sciences, Binzhou Medical University for providing experimental venues.
Funding
This work was supported by the National Natural Science Foundation of China under Grant [Grant Nos. 82172560 and 81771709], Shandong Provincial Education Department under Grant [Grant No. 2019KJK012] and Development Project of Medicine and Health Science Technology of Shandong Province under Grant [Grant No. 2019WS311].
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BL: conceived and designed study; QR: performed research, analyzed data; YD: wrote the paper; RZ: performed data collection and management; JL: contributed to revision of the manuscript; XT: contributed to revision of the manuscript; LG: contributed new methods or models; YZ: conceived of or designed study; contributed new methods/models. All authors have read and approved to the final version of the manuscript.
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The clinical gastric tissue samples used in this study were from the outpatients in the gastroscope room of the Department of Gastroenterology of Rongcheng hospital, Weihai, Shandong Province, and voluntarily participated in sample collection when the patients are informed.
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Li, B., Rong, Q., Du, Y. et al. Regulation of β1-integrin in autophagy and apoptosis of gastric epithelial cells infected with Helicobacter pylori. World J Microbiol Biotechnol 38, 12 (2022). https://doi.org/10.1007/s11274-021-03199-9
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DOI: https://doi.org/10.1007/s11274-021-03199-9