Abstract
This study aimed to examine the influence of two prebiotics, fructo-oligosaccharides (FOS) and xylo-oligosaccharides (XOS), on probiotic properties (resistance to low pH and bile salt, hydrophobicity and auto-aggregation), metabolites production, and antimicrobial activity of probiotic Lactiplantibacillus (L. pentosus S42 and L. plantarum S61) and Levilactobacillus (L. brevis S27) strains isolated from fermented olive. The results demonstrated the ability of strains to ferment XOS more than FOS as a sole carbon source, resulting in pH reduction. The prebiotics (FOS and XOS) significantly increased (p < 0.05) their survival in gastro-intestinal conditions (low pH and 0.3% of bile salts), as well as their hydrophobicity, auto-aggregation and production of proteins, compared to glucose (control). The major organic acids produced by Lactiplantibacillus and Levilactobacillus strains, were oxalic, malic and lactic acids from FOS, XOS and glucose, respectively. No antimicrobial activity was observed from cell-free supernatant (CFS) of Lactiplantibacillus and Levilactobacillus strains obtained from FOS. In the presence of XOS the organic acids, produced by Lactiplantibacillus and Levilactobacillus strains, were more diverse with high contents, and exhibited higher antifungal and antibacterial activities, more than that of FOS and glucose. The combination of L. plantarum S61 and XOS demonstrated the highest inhibition zones ranges of 20.7–22.2 mm against pathogenic bacteria and 29.2–30 mm against yeasts. This combination can be used in production of antifungal preservatives and pharmaceuticals, against pathogenic and spoilage yeasts.
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Acknowledgements
The authors are grateful to CNRST (PPR/19/2015), Canada Foundation for Innovation (CFI), Morocco-Italy cooperation (CNRST: 307/2018), and to Morocco-Tunisia cooperation (17TM06) for their support.
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AA and SK selected the study design and supervised the Lab work. HA, NK and YR conducted the experiments. AA, SK, MB, GD, RBS, ES and HA wrote the manuscript. All authors read and approved the manuscript.
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Abouloifa, H., Khodaei, N., Rokni, Y. et al. The prebiotics (Fructo-oligosaccharides and Xylo-oligosaccharides) modulate the probiotic properties of Lactiplantibacillus and Levilactobacillus strains isolated from traditional fermented olive. World J Microbiol Biotechnol 36, 185 (2020). https://doi.org/10.1007/s11274-020-02961-9
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DOI: https://doi.org/10.1007/s11274-020-02961-9