Abstract
Mycoplasma bovis (M. bovis) is an important bovine mycoplasma implicated in economically important clinical diseases, such as respiratory diseases, otitis media, and mastitis. The prevalence of M. bovis-associated mastitis in both cattle and buffaloes has been increasingly recognized as a global problem. High morbidity rates and consequential economic losses have been devastating to the affected cattle and buffalo farms, especially those in developing countries. Therefore, a rapid and accurate method is urgently needed to detect M. bovis. In this study, a rapid and simple lateral flow strip for detecting antibodies against M. bovis was established that used carbon nanoparticles (CNPs) as the labelled materials. The results from the test strip were highly consistent with those from ELISA. The test showed high specificity (100%) and no cross-reaction with other bovine pathogens. The detection sensitivity of the test was also relatively high (97.67%). All the results indicated that the colloidal carbon test strip could serve as a simple, rapid, sensitive, and specific diagnostic method for detecting antibodies against M. bovis at cattle farms.
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Andersson AM, Aspán A, Wisselink HJ et al (2019) A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis. BMC Vet Res 15:369. https://doi.org/10.1186/s12917-019-2117-0
Ayling RD, Rosales RS, Barden G, Gosney FL (2014) Changes in antimicrobial susceptibility of Mycoplasma bovis isolates from Great Britain. Vet Rec 175:486. https://doi.org/10.1136/vr.102303
Blažková M, Mičková-Holubová B, Rauch P, Fukal L (2009) Immunochromatographic colloidal carbon-based assay for detection of methiocarb in surface water. Biosens Bioelectron 25:753–758. https://doi.org/10.1016/j.bios.2009.08.023
Ball HJ, Nicholas RAJ (2010) Mycoplasma bovis-associated disease: here, there and everywhere. Vet J 186:280–281. https://doi.org/10.1016/j.tvjl.2010.02.002
Caswell JL, Archambault M (2007) Mycoplasma bovis pneumonia in cattle. Anim Health Res Rev 8:161–186. https://doi.org/10.1017/S1466252307001351
Fox LK, Kirk JH, Britten A (2005) Mycoplasma mastitis: a review of transmission and control. J Vet Med B Infect Dis Vet Public Health 52:153–160. https://doi.org/10.1111/j.1439-0450.2005.00845.x
Foster AP, Naylor RD, Howie NM, Nicholas RA, Ayling RD (2009) Mycoplasma bovis and otitis in dairy calves in the United Kingdom. Vet J 179:455–457. https://doi.org/10.1016/j.tvjl.2007.10.020
Fu P, Sun Z, Zhang Y, Yu Z, Zhang H, Su D, Jiang F, Wu W (2014) Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of p48 protein. Bmc Vet Res 10:42. https://doi.org/10.1186/1746-6148-10-42
Huang X, Aguilar ZP, Xu H, Lai W, Xiong Y (2016) Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: a review. Biosens Bioelectron 75:166–180. https://doi.org/10.1016/j.bios.2015.08.032
Hazelton MS, Morton JM, Bosward KL, Sheehy PA, Parker AM, Dwyer CJ, Niven PG, House JK (2018) Isolation of Mycoplasma spp. and serological responses in bulls prior to and following their introduction into Mycoplasma bovis-infected dairy herds. J Dairy Sci 101:7412–7424. https://doi.org/10.3168/jds.2018-14457
Jiang N, Ahmed R, Damayantharan M, Ünal B, Butt H, Yetisen AK (2019) Lateral and vertical flow assays for point-of-care diagnostics. Adv Healthcare Mater 8:2192–2640. https://doi.org/10.1002/adhm.201900244
Kim H, Chung DR, Kang M (2019) A new point-of-care test for the diagnosis of infectious diseases based on multiplex lateral flow immunoassays. Analyst 144:2460–2466. https://doi.org/10.1039/c8an02295j
Kay AW, Itoh M, Valdez J, Chen SF, Mathew R, Gans HA (2015) Pleural effusion and fever in an immunocompromised patient. J Pediat Inf Dis Soc 4:E6–E9. https://doi.org/10.1093/jpids/piu018
Li X, Qin L, Zhu H, Sun Y, Cui X, Gao Y, Qi X, Wang Y, Gao H, Gao Y, Wang X (2016) Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Arch Virol 161:2871–2877. https://doi.org/10.1007/s00705-016-2971-z
Nielsen PK, Petersen MB, Nielsen LR, Halasa T, Toft N (2015) Latent class analysis of bulk tank milk PCR and ELISA testing for herd level diagnosis of Mycoplasma bovis. Prev Vet Med 121:338–342
Noguera PS, Posthuma-Trumpie GA, Tuil MV, Wal FJVD, Boer AD, Moers APHA, Amerongen AV (2011a) Carbon nanoparticles as detection labels in antibody microarrays. Detection of genes encoding virulence factors in shiga toxin-producing Escherichia coli. Anal Chem 83:8531–8536. https://doi.org/10.1021/ac201823v
Noguera P, Posthuma-Trumpie GA, Tuil MV, Wal FJVD, Boer AD, Moers APHA, Amerongen AV (2011b) Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli. Anal Bioanal Chem 399:831–838. https://doi.org/10.1007/s00216-010-4334-z
O'Keeffe M, Crabbe P, Salden M, Wichers J, Peteghem CV, Kohen F, Pieraccini G, Moneti G (2003) Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine. J Immunol Methods 278:117–126. https://doi.org/10.1016/S0022-1759(03)00207-2
Parker AM, House JK, Hazelton MS, Bosward KL, Morton JM, Sheehy PA (2017) Bulk tank milk antibody ELISA as a biosecurity tool for detecting dairy herds with past exposure to Mycoplasma bovis. J Dairy Sci 100:8296–8309. https://doi.org/10.3168/jds.2016-12468
Parker AM, Sheehy PA, Hazelton MS, Bosward KL, House JK (2018) A review of mycoplasma diagnostics in cattle. J Vet Intern Med 32:1241–1252. https://doi.org/10.1111/jvim.15135
Suárez-Pantaleón C, Wichers J, Abad-Somovilla A, Amerongen AV, Abad-Fuentes A (2013) Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron. Biosens Bioelectron 42:170–176. https://doi.org/10.1016/j.bios.2012.11.001
Wawegama NK, Browning GF, Kanci A, Marenda MS, Markham PF (2014) Development of a recombinant protein-based enzyme-linked immunosorbent assay for diagnosis of Mycoplasma bovis infection in cattle. Clin Vaccine Immunol 21:196–202. https://doi.org/10.1128/CVI.00670-13
Zhao G, Hou P, Huan Y, He C, Wang H, He H (2018) Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis. BMC Vet Res 14:412. https://doi.org/10.1186/s12917-018-1703-x
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This study was supported by a project undertaken by Shihezi University (2017AA003). Project Name: Development of New Brucella Vaccine and Detection Reagent.
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Shi, F., Zhao, Y., Sun, Y. et al. Development and application of a colloidal carbon test strip for the detection of antibodies against Mycoplasma bovis. World J Microbiol Biotechnol 36, 157 (2020). https://doi.org/10.1007/s11274-020-02930-2
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DOI: https://doi.org/10.1007/s11274-020-02930-2