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Functioning of plant-bacterial associations under osmotic stress in vitro

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Abstract

The search for effective plant-growth-promoting strains of rhizospheric bacteria that would ensure the resistance of plant-microbial associations to environmental stressors is essential for the design of environmentally friendly agrobiotechnologies. We investigated the interaction of potato (cv. Nevsky) microplants with the plant-growth-promoting bacteria Azospirillum brasilense Sp245 and Ochrobactrum cytisi IPA7.2 under osmotic stress in vitro. The bacteria improved the physiological and biochemical variables of the microplants, significantly increasing shoot length and root number (1.3-fold, on average). Inoculation also led a more effective recovery of the plants after stress. During repair, inoculation contributed to a decreased leaf content of malonic dialdehyde. With A. brasilense Sp245, the decrease was 1.75-fold; with O. cytisi IPA7.2, it was 1.4-fold. During repair, the shoot length, node number, and root number of the inoculated plants were greater than the control values by an average of 1.3-fold with A. brasilense Sp245 and by an average of 1.6-fold with O. cytisi IPA7.2. O. cytisi IPA7.2, previously isolated from the potato rhizosphere, protected the physiological and biochemical processes in the plants under stress and repair better than did A. brasilense Sp245. Specifically, root weight increased fivefold during repair, as compared to the noninoculated plants, while chlorophyll a content remained at the level found in the nonstressed controls. The results indicate that these bacteria can be used as components of biofertilizers. A. brasilense Sp245 has favorable prospects for use in temperate latitudes, whereas O. cytisi IPA7.2 can be successfully used in saline and drought-stressed environments.

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Acknowledgements

This study was carried out under research theme no. AAAA-A17-117102740097-1. Work with plant materials was supported by the Russian Foundation for Basic Research (Grant No. 19-016-00116). Thanks are due to Mr. Dmitry N. Tychinin (IBPPM RAS) for his help with the preparation of the manuscript.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, Russian Federation, 410049

    Nina V. Evseeva, Gennady L. Burygin, Larisa Yu. Matora & Sergei Yu. Shchyogolev

  2. Vavilov Saratov State Agrarian University, 1 Teatralnaya Ploshchad, Saratov, Russian Federation, 410012

    Oksana V. Tkachenko, Alena Yu. Denisova & Gennady L. Burygin

  3. Ufa Institute of Biology, Russian Academy of Sciences, 69 Prospekt Oktyabrya, Ufa, Russian Federation, 450054

    Dmitry S. Veselov

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  1. Nina V. Evseeva
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  2. Oksana V. Tkachenko
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  3. Alena Yu. Denisova
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Correspondence to Nina V. Evseeva.

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Evseeva, N.V., Tkachenko, O.V., Denisova, A.Y. et al. Functioning of plant-bacterial associations under osmotic stress in vitro. World J Microbiol Biotechnol 35, 195 (2019). https://doi.org/10.1007/s11274-019-2778-7

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