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Development of a bacterial consortium comprising oil-degraders and diazotrophic bacteria for elimination of exogenous nitrogen requirement in bioremediation of diesel-contaminated soil

Abstract

The purpose of this study was to develop an effective bacterial consortium and determine their ability to overcome nitrogen limitation for the enhanced remediation of diesel-contaminated soils. Towards this, various bacterial consortia were constructed using oil-degrading and nitrogen-fixing microbes. The diesel removal efficiency of various developed consortia was evaluated by delivering the bacterial consortia to the diesel-contaminated soils. The consortium Acinetobacter sp. K-6 + Rhodococcus sp. Y2-2 + NH4NO3 resulted in the highest removal (85.3%) of diesel from the contaminated soil. The consortium containing two different oil-degrading microbes (K-6 + Y2-2) and one nitrogen-fixing microbe Azotobacter vinelandii KCTC 2426 removed 83.1% of the diesel from the soil after 40 days of treatment. The total nitrogen content analysis revealed higher amounts of nitrogen in soil treated with the nitrogen-fixing microbe when compared with that of the soil supplemented with exogenous inorganic nitrogen. The findings in this present study reveal that the consortium containing the nitrogen-fixing microbe degraded similar amounts of diesel to that degraded by the consortium supplemented with exogenous inorganic nitrogen. This suggests that the developed consortium K-6 + Y2-2 + KCTC 2426 compensated for the nitrogen limitation and eliminated the need for exogenous nitrogen in bioremediation of diesel-contaminated soils.

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Acknowledgements

This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant Nos. 2016R1D1A1A09916982 and 2018R1A2B6006139).

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Correspondence to Jaisoo Kim.

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Chaudhary, D.K., Bajagain, R., Jeong, SW. et al. Development of a bacterial consortium comprising oil-degraders and diazotrophic bacteria for elimination of exogenous nitrogen requirement in bioremediation of diesel-contaminated soil. World J Microbiol Biotechnol 35, 99 (2019). https://doi.org/10.1007/s11274-019-2674-1

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Keywords

  • Bacterial consortia
  • Oil-degrading microbe
  • Diazotrophic bacteria
  • Nitrogen limitation
  • Bioremediation
  • Diesel