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Carbon speciation and flocculation in Neochloris oleoabundans cultures using anaerobically digested stillage

  • Gloria Sánchez-GalvánEmail author
  • Eugenia J. Olguín
  • Alejandro A. Ceballos
  • Itzel A. Juárez
Original Paper
  • 109 Downloads

Abstract

The effects of bicarbonate loading rate (BLR) and pH on growth kinetics, inorganic carbon speciation, carbon fixation and lipid content in Neochloris oleoabundans cultures using anaerobically digested stillage (ADS) (2% v/v) were investigated. Four different cultures were established: culture A with BLR = 1 g l−1 day−1 and no pH adjustment, culture B with BLR = 0.5 g l−1 day−1 and no pH adjustment, culture C with BLR = 1 g l−1 day−1 and pH adjustment at 7.0, and culture D with BLR = 0.5 g l−1 day−1 and pH adjustment at 7.0. The experiments were carried out in flat plate reactors (4 l) at controlled conditions (light intensity of 134 µmol photon m−1 s−1 and photoperiod 16 light/8 darkness; temperature of 32 ± 1 °C). The effects of pH (7, 10.41, 10.65, and 12), time (15, 30, 60, and 90 min), and concentration of a cationic polyelectrolyte (CP) (10 and 20 mg l−1) on the flocculation efficiency (FE) of N. oleoabundans were also investigated. The results showed that bicarbonate was the predominant carbon species in the media and the main carbon source for microalgae growth in all cultures. The highest productivity (87.70 ± 9.70 mg l−1 day−1) and CO2(aq) fixation rate (0.15 g CO2(aq) l−1 day−1) were found in culture B. The lipid content in N. oleoabundans was affected negatively by the pH adjustment at 7.0 during its growth; higher values were found in cultures with no pH adjustment (37.10% and 38.85% dw for culture A and B, respectively) as compared to those obtained in cultures with pH adjustment (27.35% and 22.20% dw for culture C and D, respectively) (p < 0.05). Regarding flocculation, the addition of 20 mg CP l−1 was required to obtain a FE > 95% in cultures A and B, although a significant FE (40–59%) occurred without CP addition at a high pH (≥ 10.41) in all cultures.

Graphical abstract

Keywords

Microalgae Anaerobic effluent Carbon fixation Lipids Flocculation 

Notes

Acknowledgements

The authors thank the technical assistance of Anilú Mendoza, Karla Tapia, Alejandro Hernández and Victor Hernández-Landa.

Funding

This study was funded by the Ministry of Energy (SENER) and the National Research Council of México (CONACYT) (Project #152931).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Aishvarya V, Pradhan N, Nayak RR et al (2012) Enhanced inorganic carbon uptake by Chlorella sp. IMMTCC-2 under autotrophic conditions for lipid production and CO2 sequestration. J Appl Phycol 24(6):1455–1463.  https://doi.org/10.1007/s10811-012-9801-9 CrossRefGoogle Scholar
  2. Baicha Z, Salar-García MJ, Ortiz-Martínez VM et al (2016) A critical review on microalgae as an alternative source for bioenergy production: a promising low cost substrate for microbial fuel cells. Fuel Process Technol 154:104–116.  https://doi.org/10.1016/j.fuproc.2016.08.017 CrossRefGoogle Scholar
  3. Beach ES, Eckelman MJ, Cui Z et al (2012) Preferential technological and life cycle environmental performance of chitosan flocculation for harvesting of the green algae Neochloris oleoabundans. Bioresour Technol 121:445–449.  https://doi.org/10.1016/j.biortech.2012.06.012 CrossRefPubMedGoogle Scholar
  4. Beuckels A, Depraetere O, Vandamme D et al (2013) Influence of organic matter on flocculation of Chlorella vulgaris by calcium phosphate precipitation. Biomass Bioenergy 54:107–114.  https://doi.org/10.1016/j.biombioe.2013.03.027 CrossRefGoogle Scholar
  5. Chen L, Wang C, Wang W, Wei J (2013) Optimal conditions of different flocculation methods for harvesting Scenedesmus sp. cultivated in an open-pond system. Bioresour Technol 133:9–15.  https://doi.org/10.1016/j.biortech.2013.01.071 CrossRefPubMedGoogle Scholar
  6. Das P, Thaher MI, Hakim MAQMA et al (2016) Microalgae harvesting by pH adjusted coagulation-flocculation, recycling of the coagulant and the growth media. Bioresour Technol 216:824–829.  https://doi.org/10.1016/j.biortech.2016.06.014 CrossRefPubMedGoogle Scholar
  7. Franchino M, Comino E, Bona F, Riggio V (2013) Growth of three microalgae strains and nutrient removal from an agro-zootechnical digestate. Chemosphere 92(6):738–744.  https://doi.org/10.1016/j.chemosphere.2013.04.023 CrossRefPubMedGoogle Scholar
  8. García CFH, Souza RB, de Souza CP et al (2017) Toxicity of two effluents from agricultural activity: comparing the genotoxicity of sugar cane and orange vinasse. Ecotoxicol Environ Saf 142:216–221.  https://doi.org/10.1016/j.ecoenv.2017.03.053 CrossRefPubMedGoogle Scholar
  9. García-Pérez J, Beuckels A, Vandamme D et al (2014) Influence of magnesium concentration, biomass concentration and pH on flocculation of Chlorella vulgaris. Algal Res 3:24–29.  https://doi.org/10.1016/j.algal.2013.11.016 CrossRefGoogle Scholar
  10. Gerardo ML, Van Den Hende S, Vervaeren H et al (2015) Harvesting of microalgae within a biorefinery approach: a review of the developments and case studies from pilot-plants. Algal Res 11:248–262.  https://doi.org/10.1016/j.algal.2015.06.019 CrossRefGoogle Scholar
  11. Giordano M, Beardall J, Raven JA (2005) CO2 concentrating mechanisms in algae: mechanisms, environmental modulation, and evolution. Ann Rev Plant Biol 56:99–131.  https://doi.org/10.1146/annurev.arplant.56.032604.144052 CrossRefGoogle Scholar
  12. Goebel TS, Lascano RJ, Davis TA (2016) Phosphate sorption in water by several cationic polymer flocculants. J Agric Chem Environ 5:45–51.  https://doi.org/10.4236/jacen.2016.51005 CrossRefGoogle Scholar
  13. González-Fernández C, Molinuevo-Salces B, García-González MC (2011) Nitrogen transformations under different conditions in open ponds by means of microalgae–bacteria consortium treating pig slurry. Bioresour Technol 102(2):960–966.  https://doi.org/10.1016/j.biortech.2010.09.052 CrossRefPubMedGoogle Scholar
  14. Granados MR, Acién FG, Gómez C et al (2012) Evaluation of flocculants for the recovery of freshwater microalgae. Bioresour Technol 118:102–110.  https://doi.org/10.1016/j.biortech.2012.05.018 CrossRefPubMedGoogle Scholar
  15. Gutiérrez R, Passos F, Ferrer I et al (2015) Harvesting microalgae from wastewater treatment systems with natural flocculants: effect on biomass settling and biogas production. Algal Res 9:204–211.  https://doi.org/10.1016/j.algal.2015.03.010 CrossRefGoogle Scholar
  16. Lam MK, Lee KT (2013) Effect of carbon source towards the growth of Chlorella vulgaris for CO2 bio-mitigation and biodiesel production. Int J Greenhouse Gas Control 14:169–176.  https://doi.org/10.1016/j.ijggc.2013.01.016 CrossRefGoogle Scholar
  17. Lam MK, Lee KT, Mohamed AR (2012) Current status and challenges on microalgae-based carbon capture. Int J Greenhouse Gas Control 10:456–469.  https://doi.org/10.1016/j.ijggc.2012.07.010 CrossRefGoogle Scholar
  18. Lecina M, Nadal G, Solà C et al (2016) Optimization of ferric chloride concentration and pH to improve both cell growth and flocculation in Chlorella vulgaris cultures. Application to medium reuse in an integrated continuous culture bioprocess. Bioresour Technol 216:211–218.  https://doi.org/10.1016/j.biortech.2016.05.063 CrossRefPubMedGoogle Scholar
  19. Mehrabadi A, Craggs R, Farid MM (2016) Biodiesel production potential of wastewater treatment high rate algal pond biomass. Bioresour Technol 221:222–233.  https://doi.org/10.1016/j.biortech.2016.09.028 CrossRefPubMedGoogle Scholar
  20. Moheimani NR, Borowitzka MA, Isdepsky A, Fon-Sing S (2013) Standard methods for measuring growth of algae and their composition. In: Borowitzka MA, Moheimani NR (eds) Algae for biofuels and energy. Springer, Dordrecht, pp 265–284CrossRefGoogle Scholar
  21. Molina-Grima E, Belarbi EH, Acién-Fernández FG et al (2003) Recovery of microalgal biomass and metabolites: process options and economics. Biotechnol Adv 20:491–515.  https://doi.org/10.1016/S0734-9750(02)00050-2 CrossRefPubMedGoogle Scholar
  22. Moraes L, Martins da Rosa G, Barcelos Cardias B et al (2016) Microalgal biotechnology for greenhouse gas control: carbon dioxide fixation by Spirulina sp. at different diffusers. Ecol Eng 91:426–431.  https://doi.org/10.1016/j.ecoleng.2016.02.035 CrossRefGoogle Scholar
  23. Murray KE, Shields JA, Garcia ND, Healy FG (2012) Productivity, carbon utilization, and energy content of mass in scalable microalgae systems. Bioresour Technol 114:499–506.  https://doi.org/10.1016/j.biortech.2012.03.012 CrossRefPubMedGoogle Scholar
  24. Olguín EJ (2012) Dual purpose microalgae-bacteria-based systems that treat wastewater and produce biodiesel and chemical products within a Biorefinery. Biotechnol Adv 30(5):1031–1046.  https://doi.org/10.1016/j.biotechadv.2012.05.001 CrossRefPubMedGoogle Scholar
  25. Olguín EJ, Dorantes E, Castillo O, Hernández-Landa VJ (2015a) Anaerobic digestates from vinasse promote growth and lipid enrichment in Neochloris oleoabundans cultures. J Appl Phycol 27(5):1813–1822.  https://doi.org/10.1007/s10811-015-0540-6 CrossRefGoogle Scholar
  26. Olguín EJ, Castillo OS, Mendoza A et al (2015b) Dual purpose system that treats anaerobic effluents from pig waste and produce Neochloris oleoabundans as lipid rich biomass. New Biotechnol 32(3):387–395.  https://doi.org/10.1016/j.nbt.2014.12.004 CrossRefGoogle Scholar
  27. Pedersen O, Colmer TD, Sand-Jensen K (2013) Underwater photosynthesis of submerged plants—recent advances and methods. Front Plant Sci 4:140.  https://doi.org/10.3389/fpls.2013.00140 CrossRefPubMedPubMedCentralGoogle Scholar
  28. Peng L, Lan CQ, Zhang Z, Sarch C, Laporte M (2015) Control of protozoa contamination and lipid accumulation in Neochloris oleoabundans culture: effects of pH and dissolved inorganic carbon. Bioresour Technol 197:143–151.  https://doi.org/10.1016/j.biortech.2015.07.101 CrossRefPubMedGoogle Scholar
  29. Pezzolesi L, Samorì C, Pistocchi R (2015) Flocculation induced by homogeneous and heterogeneous acid treatments in Desmodesmus communis. Algal Res 10:145–151.  https://doi.org/10.1016/j.algal.2015.04.024 CrossRefGoogle Scholar
  30. Rasteiro MG, Pinheiro I, Ahmadloo H et al (2015) Correlation between flocculation and adsorption of cationic polyacrylamides on precipitated calcium carbonate. Chem Eng Res Des 95:298–306.  https://doi.org/10.1016/j.cherd.2014.11.007 CrossRefGoogle Scholar
  31. Roselet F, Vandamme D, Roselet M et al (2015) Screening of commercial natural and synthetic cationic polymers for flocculation of freshwater and marine microalgae and effects of molecular weight and charge density. Algal Res 10:183–188.  https://doi.org/10.1016/j.algal.2015.05.008 CrossRefGoogle Scholar
  32. Rossi F, Olguín EJ, Diels L, De Philippis R (2015) Microbial fixation of CO2 in water bodies and in drylands to combat climate change, soil loss and desertification. New Biotechnol 32(1):109–120.  https://doi.org/10.1016/j.nbt.2013.12.002 CrossRefGoogle Scholar
  33. Santos AM, Janssen M, Lamers PP et al (2012) Growth of oil accumulating microalga Neochloris oleoabundans under alkaline–saline conditions. Bioresour Technol 104:593–599.  https://doi.org/10.1016/j.biortech.2011.10.084 CrossRefPubMedGoogle Scholar
  34. Sirin S, Trobajo R, Ibanez C, Salvadó J (2012) Harvesting the microalgae Phaeodactylum tricornutum with polyaluminum chloride, aluminium sulphate, chitosan and alkalinity-induced flocculation. J Appl Phycol 24:1067–1080.  https://doi.org/10.1007/s10811-011-9736-6 CrossRefGoogle Scholar
  35. Sun Y, Ren M, Zhu C et al (2016) UV-initiated graft copolymerization of cationic chitosan-based flocculants for treatment of zinc phosphate-contaminated wastewater. Ind Eng Chem Res 55(38):10025–10035.  https://doi.org/10.1021/acs.iecr.6b02855 CrossRefGoogle Scholar
  36. Tang D, Han W, Li P et al (2011) CO2 biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels. Bioresour Technol 102(3):3071–3076.  https://doi.org/10.1016/j.biortech.2010.10.047 CrossRefPubMedGoogle Scholar
  37. ’t Lam GP, Zegeye EK, Vermue MH et al (2015) Dosage effect of cationic polymers on the flocculation efficiency of the marine microalga Neochloris oleoabundans. Bioresour Technol 198:797–802.  https://doi.org/10.1016/j.biortech.2015.09.097 CrossRefPubMedGoogle Scholar
  38. ’t Lam GP, Giraldo JB, Vermuë MH et al (2016) Understanding the salinity effect on cationic polymers in inducing flocculation of the microalga Neochloris oleoabundans. J Biotechnol 225:10–17.  https://doi.org/10.1016/j.jbiotec.2016.03.009 CrossRefPubMedGoogle Scholar
  39. Uggetti E, Sialve B, Latrille E, Steyer JP (2014) Anaerobic digestate as substrate for microalgae culture: the role of ammonium concentration on the microalgae productivity. Bioresour Technol 152:437–443.  https://doi.org/10.1016/j.biortech.2013.11.036 CrossRefPubMedGoogle Scholar
  40. Vandamme D, Foubert I, Fraeye I et al (2012) Flocculation of Chlorella vulgaris induced by high pH: role of magnesium and calcium and practical implications. Bioresour Technol 105:114–119.  https://doi.org/10.1016/j.biortech.2011.11.105 CrossRefPubMedGoogle Scholar
  41. Wan C, Alam MA, Zhao XQ et al (2015) Current progress and future prospect of microalgal biomass harvest using various flocculation technologies. Bioresour Technol 184:251–257.  https://doi.org/10.1016/j.biortech.2014.11.081 CrossRefPubMedGoogle Scholar
  42. Yun HS, Ji MK, Park YT et al (2016) Microalga, Acutodesmus obliquus KGE 30 as a potential candidate for CO2 mitigation and biodiesel production. Environ Sci Pollut Res 23(17):17831–17839.  https://doi.org/10.1007/s11356-016-6971-z CrossRefGoogle Scholar
  43. Zhang H, Wang L, Sommerfeld M, Hu Q (2016) Harvesting microalgal biomass using magnesium coagulation-dissolved air flotation. Biomass Bioenergy 93:43–49.  https://doi.org/10.1016/j.biombioe.2016.06.024 CrossRefGoogle Scholar
  44. Zhang A, Sun D, Wu T et al (2017) The synergistic energy and carbon metabolism under mixotrophic cultivation reveals the coordination between photosynthesis and aerobic respiration in Chlorella zofingiensis. Algal Res 25:109–116.  https://doi.org/10.1016/j.algal.2017.05.007 CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Environmental Biotechnology GroupInstitute of EcologyXalapaMexico

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