Screening of plant growth promotion ability among bacteria isolated from field-grown sorghum under different managements in Brazilian drylands

da Silva, Jéssica Fernanda; da Silva, Thaise Rosa; Escobar, Indra Elena Costa; Fraiz, Ana Carla Resende; dos Santos, Jonnathan Whiny Moraes; do Nascimento, Tailane Ribeiro; dos Santos, João Marcos Rodrigues; Peters, Samuel James Windsor; de Melo, Roseli Freire; Signor, Diana; Fernandes-Júnior, Paulo Ivan

doi:10.1007/s11274-018-2568-7

Original Paper
Published: 30 November 2018

Screening of plant growth promotion ability among bacteria isolated from field-grown sorghum under different managements in Brazilian drylands

Jéssica Fernanda da Silva¹,
Thaise Rosa da Silva¹,
Indra Elena Costa Escobar¹,
Ana Carla Resende Fraiz¹,
Jonnathan Whiny Moraes dos Santos²,
Tailane Ribeiro do Nascimento³,
João Marcos Rodrigues dos Santos⁴,
Samuel James Windsor Peters⁵,
Roseli Freire de Melo⁶,
Diana Signor⁶ &
Paulo Ivan Fernandes-Júnior ORCID: orcid.org/0000-0002-6390-3720⁶

World Journal of Microbiology and Biotechnology volume 34, Article number: 186 (2018) Cite this article

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Abstract

Sorghum [Sorghum bicolor (L.) Moench] is a multipurpose grass cultivated in drylands due to its adaptation to drought. However the characteristics of sorghum-associated bacteria are not known in the Brazilian drylands. The aim of this study was to isolate and evaluate the plant growth promotion potential bacteria from field-grown sorghum under two irrigation and manure application levels in a Brazilian semi-arid reagion. Sorghum was irrigated with 3 or 1 mm day⁻¹ and fertilized or not with liquid goat manure. Bacteria were obtained from surface-disinfected roots applying two nitrogen-free semi-solid media. The bacteria were evaluated for the presence of nifH gene, 16S rRNA sequences, calcium-phosphate solubilization, production of auxins and siderophores and for sorghum growth promotion. We obtained 20 out of 24 positive bacteria for nifH. The isolates were classified as in six different genera. All isolates produced auxins “in vitro”, six bacteria produced siderophores and three Enterobacteriaceae solubilized calcium-phosphate. At least ten bacteria resulted in the increased total N content in the sorghum shoots, comparable to fertilization with 50 mg N plant⁻¹ week⁻¹ and to inoculation with Azospirillum brasilense Ab-V5. Enterobacter sp. ESA 57 was the best sorghum plant-growth promoting bacteria isolated in this study.

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Acknowledgements

Acknowledgments are given to Brazilian Council for Scientific and Technological Development (CNPq 485168/2013-8), to INCT—Plant Growth Promoting Microorganisms for Agricultural Sustainability and Environmental Responsibility (CNPq/Fundação Araucária INCT-MPCPAgro 465133/2014-4) and the Brazilian Agricultural Research Corporation (Embrapa 03.13.08.003.00.00 and 06.13.06.002.00.00) by financial support. Acknowledgments are also given to the Science foundation of the Pernambuco State (FACEPE), for the scholarship to the first and second authors, and to Coordination of Improvement of Higher Education Personnel (CAPES) for providing the scholarship for the third to seventh authors. The last author is a research fellow of CNPq (311218/2017-2).

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Authors and Affiliations

Universidade Federal do Vale do São Francisco, Colegiado de Farmácia, Av. José de Sá Maniçoba, s/n, Petrolina, PE, Brazil
Jéssica Fernanda da Silva, Thaise Rosa da Silva, Indra Elena Costa Escobar & Ana Carla Resende Fraiz
Universidade Estadual da Paraíba, R. Baraúnas, 351, Campina Grande, PB, Brazil
Jonnathan Whiny Moraes dos Santos
Universidade do Estado da Bahia, Campus III, Departamento de Tecnologia e Ciências Sociais, R. Edgard Chastinet, s/n, Juazeiro, BA, Brazil
Tailane Ribeiro do Nascimento
Universidade Federal do Ceará, Campus do Pici, Av. Mister Hull, 2977, Fortaleza, CE, Brazil
João Marcos Rodrigues dos Santos
Emory University, Dowman Drive, 201, Atlanta, GA, USA
Samuel James Windsor Peters
Embrapa Semiárido, BR 428, km 152, s/n, Petrolina, PE, Brazil
Roseli Freire de Melo, Diana Signor & Paulo Ivan Fernandes-Júnior

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Jéssica Fernanda da Silva
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Thaise Rosa da Silva
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Indra Elena Costa Escobar
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Ana Carla Resende Fraiz
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Jonnathan Whiny Moraes dos Santos
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Tailane Ribeiro do Nascimento
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João Marcos Rodrigues dos Santos
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Samuel James Windsor Peters
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Roseli Freire de Melo
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Diana Signor
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Paulo Ivan Fernandes-Júnior
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Correspondence to Paulo Ivan Fernandes-Júnior.

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da Silva, J.F., da Silva, T.R., Escobar, I.E.C. et al. Screening of plant growth promotion ability among bacteria isolated from field-grown sorghum under different managements in Brazilian drylands. World J Microbiol Biotechnol 34, 186 (2018). https://doi.org/10.1007/s11274-018-2568-7

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Received: 21 June 2018
Accepted: 25 November 2018
Published: 30 November 2018
DOI: https://doi.org/10.1007/s11274-018-2568-7

Keywords

Biological nitrogen fixation
Inoculant
Enterobacteriaceae
Plant growth promotion