Environmental proteomic studies: closer step to understand bacterial biofilms
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Advancement in proteome analytical techniques and the development of protein databases have been helping to understand the physiology and subtle molecular mechanisms behind biofilm formation in bacteria. This review is to highlight how the evolving proteomic approaches have revealed fundamental molecular processes underlying the formation and regulation of bacterial biofilms. Based on the survey of research reports available on differential expression of proteins in biofilms of bacterial from wide range of environments, four important cellular processes viz. metabolism, motility, transport and stress response that contribute to formation of bacterial biofilms are discussed. This review might answer how proteins related to these cellular processes contribute significantly in stabilizing biofilms of different bacteria in diverse environmental conditions.
KeywordsBacteria Biofilm Proteomics Tools Techniques
Sodium dodecyl sulphate polyacrylamide gel electrophoresis
Two dimensional polyacrylamide gel electrophoresis
Isotope coded affinity tag
Isobaric tag for relative and absolute quantitation
Tandem mass tags
Acid mine drainage
- 3D, OD
Difference gel electrophoresis
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
Electrospray ionization-quadrupole ion trap mass spectrometry
Nano liquid chromatography-mass spectrometry
- LC-ESI, TCA
Tricarboxylic acid cycle
Bis-(3′-5′)-cyclic dimeric guanosine monophosphate
Tripartite ATP-independent periplasmic
Reactive oxygen species
Outer membrane vesicles
The authors gratefully acknowledge the support rendered by the management of Vellore Institute of Technology, Vellore, India in carrying out their research.
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interests.
Research involving human participants and/or animals
The research work does not involve human participants and/or animals.
The research work does not involve human participants and hence informed consent does not arise.
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