Abstract
GTPase activation protein (GAP) for Rab GTPases can accelerate GTP hydrolysis to alter the activity of Rab GTPases. To explore the function of GAP in entomopathogenic fungi, we constructed a deletion mutant of Gyp2 gene, a member of the Gyp (GAP for Ypt/Rab proteins) family in the locust-specific fungal pathogen, Metarhizium acridum. Results showed that the ∆MaGyp2 mutant had dramatically decreased tolerance to ultraviolet irradiation compared to wild type strain. Quantitative real-time PCR revealed that UV irradiation repair related genes Uve1 and WC1 were downregulated in ∆MaGyp2 mutant. Seven of other ten Gyp family members had significantly increased transcription in ∆MaGyp2 mutant compared with wild type, which may partly rescue the deficiency of MaGyp2.
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This work was financially supported by the Natural Science Foundation of China (Nos. 31371992, 31772222).
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Xie, M., Xia, Y. & Cao, Y. The Rab GTPase activating protein Gyp2 contributes to UV stress tolerance in Metarhizium acridum. World J Microbiol Biotechnol 34, 78 (2018). https://doi.org/10.1007/s11274-018-2457-0
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DOI: https://doi.org/10.1007/s11274-018-2457-0