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Cell regeneration and cyclic catalysis of engineered Kluyveromyces marxianus of a d-psicose-3-epimerase gene from Agrobacterium tumefaciens for d-allulose production

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Abstract

d-Allulose as a low-energy and special bioactive monosaccharide sugar is essential for human health. In this study, the d-psicose-3-epimerase gene (DPEase) of Agrobacterium tumefaciens was transferred into thermotolerant Kluyveromyces marxianus to decrease the production cost of d-allulose and reduce the number of manufacturing procedures. The cell regeneration of K. marxianus and cyclic catalysis via whole-cell reaction were investigated to achieve the sustainable application of K. marxianus and the consumption of residual d-fructose. Results showed that DPEase, encoding a 33 kDa protein, could be effectively expressed in thermotolerant K. marxianus. The engineered K. marxianus produced 190 g L−1 d-allulose with 750 g L−1 d-fructose as a substrate at 55 °C within 12 h. Approximately 100 g of residual d-fructose was converted into 34 g of ethanol, and 15 g of the engineered K. marxianus cells was regenerated after fermentation at 37 °C for 21 h. The purity of d-allulose of more than 90% could be obtained without isolating it from d-allulose and d-fructose mixture through residual d-fructose consumption. This study provided a valuable pathway to regenerate engineered K. marxianus cells and achieve cyclic catalysis for d-allulose production.

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Funding

This study was funded by Key Science and Technology Program of Anhui Province (No.1604a0702001).

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Authors and Affiliations

  1. College of Food Science and Engineering, Anhui Key Laboratory of Intensive Processing of Agricultural Products, Hefei University of Technology, Tunxi Road 193, Hefei, 230009, Anhui, China

    Peizhou Yang, Xingxing Zhu, Zhi Zheng, Dongdong Mu, Shaotong Jiang, Shuizhong Luo, Yun Wu & Minrui Du

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  1. Peizhou Yang
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  2. Xingxing Zhu
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  3. Zhi Zheng
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  4. Dongdong Mu
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  5. Shaotong Jiang
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  6. Shuizhong Luo
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  7. Yun Wu
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  8. Minrui Du
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Corresponding author

Correspondence to Peizhou Yang.

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Fig. S1

Skeletal structure of the constructed pRS42H-dpe plasmid (TIF 560 KB)

Fig. S2

Effect of hygromycin concentrations on the growth of K. marxianus on the solid YPD plate (TIF 1223 KB)

Supplementary material 3 (DOC 57 KB)

Supplementary material 4 (DOC 65 KB)

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Yang, P., Zhu, X., Zheng, Z. et al. Cell regeneration and cyclic catalysis of engineered Kluyveromyces marxianus of a d-psicose-3-epimerase gene from Agrobacterium tumefaciens for d-allulose production. World J Microbiol Biotechnol 34, 65 (2018). https://doi.org/10.1007/s11274-018-2451-6

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  • DOI: https://doi.org/10.1007/s11274-018-2451-6

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