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Metatranscriptomics analysis of mangroves habitats around Mauritius

  • Sillma Rampadarath
  • Kushlata Bandhoa
  • Daneshwar Puchooa
  • Rajesh Jeewon
  • Subhasisa Bal
Original Paper

Abstract

Mangroves are unique but endangered coastal ecosystems that play a vital role in the tropical and subtropical environments. Mauritius has two species of mangroves, Bruguiera gymnorrhiza (L.) Lam. and Rhizophora mucronata Lam., growing along its coast. The mangrove rhizosphere harbours a diverse microbial community and the use of RNA-sequencing can reveal both the taxonomic composition and active biochemical functions of the complex microbial community. Metatranscriptomic study was carried out by comparing the microbial community of rhizosphere microbiomes sediments from the two mangroves species. The study also included a comparison between a natural and a man grown mangrove microbiome. Overall, samples showed predominance by Proteobacteria, Bacteroidetes and Firmicutes, with high abundance of sulphate reducers, nitrogen reducers and methanogens. Significant difference was, however, noted at both taxonomic and functional levels among the mangroves species. The data also indicate that the microbial core involved in methane, nitrogen, and sulphur metabolism consisted mainly of Burkholderiaceae, Planctomycetaceae, Rhodobacteraceae, and Desulfobacteraceae. Also, genes encoding enzymes involved in carbon cycling, the metabolism of nitrogen, methane and sulphur were dominant in the rhizosphere of the natural mangrove ecosystem. To our knowledge, this is a first metatranscriptomic study on the microbiome of mangroves in the Mauritius, and our results provide the first insights in the range of functions and microbial diversity of the local mangrove species.

Keywords

Bruguiera gymnorrhiza Enzymes Genes Mangroves Metatranscriptomics Rhizophora mucronate 

Notes

Acknowledgements

The authors wish to thank Chowdhoory Pravin for the collection of samples. This work was supported by the University of Mauritius (Grant: 0085) and LANDRACE (Surya Seeds Research).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Agriculture and Food Science, Faculty of AgricultureUniversity of MauritiusRéduitMauritius
  2. 2.Department of Health Science, Faculty of ScienceUniversity of MauritiusRéduitMauritius
  3. 3.LANDRACE (Surya Seeds Research) Garh-GuatiraPuriIndia

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