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Screening and identification of horticultural soil fungi for their evaluation against the plant parasitic nematode Nacobbus aberrans

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Abstract

The plant-parasitic nematode Nacobbus aberrans is an endoparasite causing severe losses to a wide range of crops from North to South America. The use of native antagonistic fungi may be considered as a possible biological control alternative to reduce the damages caused by this species. Antagonistic effects of 66 potential nematophagous fungi against eggs (J1) and second-stage juveniles (J2) of N. aberrans, were evaluated in vitro on water agar. DGC test showed significant differences (p < 0.0001) in the efficacy of some fungal isolates tested, with parasitism levels for J1 and J2 of 0–95 and 1–78%, respectively. Five isolates of Purpureocillium lilacinum, Metarhizium robertsii and Plectosphaerella plurivora appeared as the most effective antagonists of N. aberrans, relying on hyphae and adhesive conidia in host infection processes.

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Acknowledgements

This work was carried out by the grant from Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT), FONCYT-PICT/2016 No. 1005, 2017–2020 and Secretaría de Ciencia y Técnica, Universidad Nacional de Río Cuarto (SECYT-UNRC), PPI-2016 Res. 161, 2016–2018. The authors are grateful for the technical assistance of Dr. Alberto Troccoli, Istituto per la Protezione Sostenibile delle Piante, CNR, Bari, Italy.

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Authors and Affiliations

  1. CONICET – Laboratorio de Ecología Microbiana, Universidad Nacional de Río Cuarto (UNRC), Río Cuarto, Argentina

    Ana L. Sosa, Miriam G. Etcheverry & María A. Passone

  2. CNR - Istituto per la Protezione Sostenibile delle Piante, Sede di Bari, via G. Amendola, 122/D, 70126, Bari, Italy

    Laura C. Rosso

  3. Cátedra de Horticultura, Universidad Nacional de Río Cuarto (UNRC), Río Cuarto, Argentina

    Fabricio A. Salusso

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  1. Ana L. Sosa
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  2. Laura C. Rosso
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  3. Fabricio A. Salusso
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  4. Miriam G. Etcheverry
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  5. María A. Passone
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Correspondence to María A. Passone.

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Sosa, A.L., Rosso, L.C., Salusso, F.A. et al. Screening and identification of horticultural soil fungi for their evaluation against the plant parasitic nematode Nacobbus aberrans. World J Microbiol Biotechnol 34, 63 (2018). https://doi.org/10.1007/s11274-018-2441-8

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