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Oil-degrading properties of a psychrotolerant bacterial strain, Rhodococcus sp. Y2-2, in liquid and soil media

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Abstract

The aim of this study was to investigate oil-degrading ability of newly isolated strain Rhodococcus Y2-2 at low temperature. Rhodococcus sp. Y2-2 was isolated from oil-contaminated soil sampled at the end of winter using a newly developed transwell plate method. In the liquid phase, the oil-degradation efficiency of strain Rhodococcus sp. Y2-2 was about 84% with an initial concentration of 1500 ppm TPH (500 ppm each of kerosene, gasoline, and diesel) when incubated for 2 weeks under optimal conditions: 10 °C, pH 7, and 0.5 g L− 1 inoculum. In the soil phase, the isolate showed 80% oil degradation efficiency using glucose as a carbon source, with an initial concentration of 4000 ppm TPH and the addition of water during 14 days of incubation at 10 °C. Additionally, the degradation efficiency of the isolate was increased by the addition of mixture of surfactant alpha olefin sulfonate and gelatin, although strain Y2-2 also produced many biosurfactant components. This study shows Rhodococcus sp. Y2-2 can degrade oil components both in liquid and soil media by consuming kerosene, gasoline, and diesel as a carbon and energy source. Therefore, the crude oil-degrading ability of Rhodococcus sp. Y2-2 at low temperature provides proper bioremediation tool to clean up oil-contaminated sites especially in cold area or during winter season.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A01059664 and 2016R1D1A1A09916982).

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Author notes

  1. Van Hong Thi Pham and Dhiraj Kumar Chaudhary were equally contributed for this article as First authors.

Authors and Affiliations

  1. Ecology Laboratory, Department of Life Science, College of Natural Sciences and Engineering, Kyonggi University, 154-42 Gwanggyosan-Ro, Youngtong-Gu, Suwon, Gyeonggi-Do, 16227, South Korea

    Van Hong Thi Pham, Dhiraj Kumar Chaudhary & Jaisoo Kim

  2. Department of Environmental Engineering, Kunsan National University, Kunsan, South Korea

    Seung-Woo Jeong

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  1. Van Hong Thi Pham
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  2. Dhiraj Kumar Chaudhary
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  3. Seung-Woo Jeong
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  4. Jaisoo Kim
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Correspondence to Jaisoo Kim.

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Van Hong Thi Pham, Chaudhary, D.K., Jeong, SW. et al. Oil-degrading properties of a psychrotolerant bacterial strain, Rhodococcus sp. Y2-2, in liquid and soil media. World J Microbiol Biotechnol 34, 33 (2018). https://doi.org/10.1007/s11274-018-2415-x

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