Archaeal community compositions in tilapia pond systems and their influencing factors
Archaea, like the bacterial communities are gradually being realized as key players in the biogeochemical progress of water ecosystems. In this study, tilapia aquaculture ponds were used for an in-depth understanding of archaeal community compositions in water and surface sediment. Some of the main functions, as well as the communities’ response patterns, to time variations, pond differences and some physio-chemical parameters were investigated. The results revealed the dominant phylum in both the water and surface sediment, as Euryarchaeota, while, the most abundant classes were: Halobacteria and Methanomicrobia respectively. Significant differences in the archaeal community compositions in the water and surface sediment, were observed in the early stages of cultivation, which became minimal at the later stage of the GIFT tilapia cultivation. Additionally to the differences in the most abundant classes, more OTUs were observed in water samples than in surface sediment samples. The methane generation could be attributed to the large proportion of methanogens found in both pond water and in the surface sediment. Furthermore, the archaeal community compositions in water and the surface sediment were shaped mainly by temporal variations and pond differences respectively. In the pond water, the archaeal community compositions were highly co-related to the concentration changes of ammonia, sulfate and total nitrogen; while in the surface sediment, the correlation to the content changes was significant in total phosphorus. The archaeal community compositions in surface sediment should be considered as an indicator for future environmental capacity studies in aquaculture.
KeywordsTilapia pond Archaeal community Illumina high-throughput sequencing Influencing factors
This research was jointly supported by the China Agriculture Research System (Grant CARS-46) and the Special Fund of Fundamental Scientific Research Business Expense of the Central Public Research Institutes (Grant 2015JBFM12).
- Guyader J, Silberberg M, Popova M, Seradj AR, Morgavi D, Martin C (2014) Dietary nitrates decrease methane emission by inhibiting rumen methanogenic archaea without influencing nitrate reducing bacteria. In: Proceedings of the 9th Joint Rowett/INRA symposium, gut microbiology: from sequence to function, Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, p 13Google Scholar
- Lu S, Liu X, Ma Z, Liu Q, Wu Z, Zeng X, Shi X, Gu Z (2015b) Vertical segregation and phylogenetic characterization of ammonia-oxidizing bacteria and archaea in the sediment of a freshwater aquaculture pond. Front Microbiol 6:177–183Google Scholar
- Pesce S, Bouchez A, Montuelle B (2011) Effects of organic herbicides on phototrophic microbial communities in freshwater ecosystems. Rev Environ Contam Toxicol 214:87–124Google Scholar
- R Development Core Team (2011) R: a language and environment for statistical computing, 2.13.1 ed. R Foundation for Statistical Computing, Vienna, AustriaGoogle Scholar
- Rafael D (2008) Tilapia farming: a global review (1924–2004). Asia Life Sci 17:207–229Google Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541CrossRefGoogle Scholar
- Tomoko S, Yoshimi F, Toru S (2008) Comparison of microbial community structures in intensive and extensive shrimp culture ponds and a mangrove area in Thailand. Fish Sci 74:889–898Google Scholar
- Zhang M, Liao M, Dapeng LI, Shimin LU, Chen J, Xugang HE (2013) Effects of three kinds of antibiotic on the nitrification and the growth of ammonia-oxidizing microorganism in freshwater aquaculture pond sediment. Fish Modernization 3:25–36 (in Chinese)Google Scholar
- Zhang DP, Wei M, Qiu QF, Wang CL (2016) Seasonal variations of ammonia-oxidizing archaea in two kinds of ponds of Portunus trituberculatus. J Biol 1:21–26 (in Chinese)Google Scholar