Abstract
The study of metal-tolerant bacteria is important for bioremediation of contaminated environments and development of green technologies for material synthesis due to their potential to transform toxic metal ions into less toxic compounds by mechanisms such as reduction, oxidation and/or sequestration. In this study, we report the isolation of seven lead-tolerant bacteria from a metal-contaminated site at Zacatecas, México. The bacteria were identified as members of the Staphylococcus and Bacillus genera by microscopic, biochemical and 16S rDNA analyses. Minimal inhibitory concentration of these isolates was established between 4.5 and 7.0 mM of Pb(NO3)2 in solid and 1.0–4.0 mM of Pb(NO3)2 in liquid media. A quantitative analysis of the lead associated to bacterial biomass in growing cultures, revealed that the percentage of lead associated to biomass was between 1 and 37% in the PbT isolates. A mechanism of complexation/biosorption of lead ions as inorganic phosphates (lead hydroxyapatite and pyromorphite) in bacterial biomass, was determined by Fourier transform infrared spectroscopy and X-ray diffraction analyses. Thus, the ability of the lead-tolerant isolates to transform lead ions into stable and highly insoluble lead minerals make them potentially useful for immobilization of lead in mining waste.
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Acknowledgements
We thanks and appreciate the technical support of the M.D. Beatriz A. Rivera Escoto from Laboratorio Nacional de Investigaciones en Nanociencias y Nanotecnología (LINAN-IPICYT). The authors would like to express their gratitude to Consejo Nacional de Ciencia y Tecnología (CONACyT) for the financial support and scholarship for V.R.S. (Grants LINAN-0271911 and FOMIX-ZAC-2013-C01-202597).
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Rodríguez-Sánchez, V., Guzmán-Moreno, J., Rodríguez-González, V. et al. Biosorption of lead phosphates by lead-tolerant bacteria as a mechanism for lead immobilization. World J Microbiol Biotechnol 33, 150 (2017). https://doi.org/10.1007/s11274-017-2314-6
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DOI: https://doi.org/10.1007/s11274-017-2314-6