Skip to main content
Springer Nature Link
Log in

Environmental yeast communities in vineyards in the mountains of Santa Catarina State, Brazil

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Yeasts were isolated from three vineyards located in the South Region of Brazil. A cross evaluation was carried out at the oldest vineyard of the study in Pinheiro Preto. Samples of grape berries, grapevine leaves and the soil, along with samples of the winery equipment and effluent, were collected. In the Serra do Marari and Campos Novos vineyards only samples of grape clusters were obtained. The 106 yeast isolates were identified by sequencing the D1/D2 domain of LSU rDNA or ITS1-5.8S-ITS2 region in 22 species. The values for the richness indices varied between the vineyards. A comparison of the taxonomic diversity of the yeasts from these regions using the reciprocal Simpson index showed a significant difference between the Serra do Marari and Campos Novos vineyards (5.72 ± 0.36 and 2.92 ± 0.36, respectively, p < 0.0001). The functional diversity was assessed in relation to the use of carbon and nitrogen sources by the yeasts isolated from each location. In general, we observed that the Pinheiro Preto and Campos Novos vineyards differed consistently from the Serra do Marari vineyard according to these indices (FAD2, FDc and Rao, p < 0.0001). The possible spreading of Saccharomyces cerevisiae from the winery to the vineyard in Pinheiro Preto was observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Badotti F, Goulart Dário M, Rosa CA, Ugarte Stambuk B (2010) Caracterização bioquímica e molecular de linhagens de Saccharomyces cerevisiae isoladas de fermentações artesanais de caldo de cana e melado para a produção de cachaça em Florianópolis-SC. Braz J Food Technol 13:205–213

    Article  CAS  Google Scholar 

  • Badotti F, Vilaça ST, Arias A, Rosa CA, Barrio E (2014) Two interbreeding populations of Saccharomyces cerevisiae strains coexist in cachaça fermentations from Brazil. FEMS Yeast Res 14:289–301

    Article  CAS  Google Scholar 

  • Barata A, González S, Malfeito-Ferreira M, Querol A, Loureiro V (2008) Sour rot-damaged grapes are sources of wine spoilage yeasts. FEMS Yeast Res 8:1008–1017

    Article  CAS  Google Scholar 

  • Cadez N, Zupan J, Raspor P (2010) The effect of fungicides on yeast communities associated with grape berries. FEMS Yeast Res 10:619–630

    CAS  Google Scholar 

  • Cappello MS, Bleve G, Grieco F, Dellaglio F, Zacheo G (2004) Characterization of Saccharomyces cerevisiae strains isolated from must of grape grown in experimental vineyard. J Appl Microbiol 97:1274–1280

    Article  CAS  Google Scholar 

  • Casanoves F, Pla L, Di Rienzo JA, Díaz S (2011) FDiversity: a software package for the integrated analysis of functional diversity. Methods Ecol Evol 2:233–237

    Article  Google Scholar 

  • Clavijo A, Calderón IL, Paneque P (2010) Diversity of Saccharomyces and non-Saccharomyces yeasts in three red grape varieties cultured in the Serranía de Ronda (Spain) vine-growing region. Int J Food Microbiol 143:241–245

    Article  CAS  Google Scholar 

  • Colwell RK, Chao A, Gotelli NJ, Lin S-Y, Mao CX, Chazdon RL, Longino JT (2012) Models and estimators linking individual-based and sample-based rarefaction, extrapolation and comparison of assemblages. J Plant Ecol 5:3–21

    Article  Google Scholar 

  • Combina M, Mercado L, Borgo P, Elia A, Jofré V, Ganga A, Martinez C, Catania C (2005) Yeasts associated to Malbec grape berries from Mendoza, Argentina. J Appl Microbiol 98:1055–1061

    Article  CAS  Google Scholar 

  • Comitini F, Ciani M, Politecnica U, Bianche VB (2008) Influence of fungicide treatments on the occurrence of yeast flora associated with wine grapes. Ann Microbiol 58(3):489–493

    Article  Google Scholar 

  • De Freitas WK, Mauro L, Magalhães S (2012) Métodos e Parâmetros para Estudo da Vegetação com Ênfase no Estrato Arbóreo Methods and Parameters for Plant Survey with Emphasis on Arboreal Stratum. Floresta e Ambiente 19(4):520–540

    Article  Google Scholar 

  • Del Mónaco SM, Barda NB, Rubio NC, Caballero AC (2014) Selection and characterization of a Patagonian Pichia kudriavzevii for wine deacidification. J Appl Microbiol 117(2):451–464

    Article  Google Scholar 

  • Ferraz ACP, Gadelha BDQ, Aguiar-coelho VM (2009) Análise faunística de Calliphoridae (Diptera) da Reserva Biológica Ferraz Tinguá, Nova Iguaçu, Rio de Janeiro. Rev Bras Entomol 53(4):620–628

    Article  Google Scholar 

  • Fleet G (2003) Yeast interactions and wine flavour. Int J Food Microbiol 86:11–22

    Article  CAS  Google Scholar 

  • Greppi A, Rantisou K, Padonou W, Hounhouigan J, Jespersen L, Jakobsen M, Cocolin L (2013) Yeast dynamics during spontaneous fermentation of mawè and tchoukoutou, two traditional products from Benin. Int J Food Microbiol 165:200–207

    Article  CAS  Google Scholar 

  • Guerra JB, Araújo R a, Pataro C, Franco GR, Moreira ES, Mendonça-Hagler LC, Rosa CA (2001) Genetic diversity of Saccharomyces cerevisiae strains during the 24 h fermentative cycle for the production of the artisanal Brazilian cachaça. Lett Appl Microbiol 33:106–111

    Article  CAS  Google Scholar 

  • Hong Y-A, Park H-D (2013) Role of non-Saccharomyces yeasts in Korean wines produced from Campbell Early grapes: potential use of Hanseniaspora uvarum as a starter culture. Food Microbiol 34:207–214

    Article  CAS  Google Scholar 

  • Hyma KE, Fay JC (2013) Mixing of vineyard and oak-tree ecotypes of Saccharomyces cerevisiae in North American vineyards. Mol Ecol 22:2917–2930

    Article  Google Scholar 

  • Libkind D, Moliné M, Sampaio JP, Van Broock M (2009) Yeasts from high-altitude lakes: influence of UV radiation. FEMS Microbiol Ecol 69:353–362

    Article  CAS  Google Scholar 

  • Marini L, Fontana P, Scotton M, Klimek S (2007) Vascular plant and Orthoptera diversity in relation to grassland management and landscape composition in the European Alps. J Appl Ecol 45:361–370

    Article  Google Scholar 

  • Oliveros JC (2007–2015) Venny. An interactive tool for comparing lists with Venn’s diagrams. http://bioinfogp.cnb.csic.es/tools/venny/index.html

  • Osorio-cadavid E, Ramírez M (2009) Estandarización de un protocolo sencillo para la extracción de ADN genómico de levaduras (Standardising a simple protocol for extracting yeast from genomic DNA). Rev Colomb Biotecnol 11(1):125–131

    Google Scholar 

  • Palanca L, Gaskett AC, Günther CS, Newcomb RD, Goddard MR (2013) Quantifying variation in the ability of yeasts to attract Drosophila melanogaster. PLoS ONE 8:e75332

    Article  CAS  Google Scholar 

  • Pallmann P, Schaarschmidt F, Hothorn LA, Nacke H, Priesnitz KAIU (2012) Assessing group differences in biodiversity by simultaneously testing a user-defined selection of diversity indices. Mol Ecol Resour 12:1068–1078

    Article  Google Scholar 

  • Pfeiffer M, Mezger D (2012) Biodiversity assessment in incomplete inventories: leaf litter ant communities in several types of Bornean rain forest. PLoS ONE 7:e40729

    Article  CAS  Google Scholar 

  • Pinto C, Pinho D, Sousa S, Pinheiro M, Egas C, Gomes AC (2014) Unravelling the diversity of grapevine microbiome. PLoS ONE 9:1–12

    Google Scholar 

  • Prakitchaiwattana CJ, Fleet GH, Heard GM (2004) Application and evaluation of denaturing gradient gel electrophoresis to analyse the yeast ecology of wine grapes. FEMS Yeast Res 4:865–877

    Article  CAS  Google Scholar 

  • Ramírez-Castrillón M, Mendes SDC, Inostroza-Ponta M, Valente P (2014) (GTG)5 MSP-PCR fingerprinting as a technique for discrimination of wine associated yeasts? PLoS ONE 9:e105870

    Article  Google Scholar 

  • Raspor P, Milek DM, Polanc J, Mozina SS, Cadez N (2006) Yeasts isolated from three varieties of grapes cultivated in different locations of the Dolenjska vine-growing region, Slovenia. Int J Food Microbiol 109:97–102

    Article  CAS  Google Scholar 

  • Sadoudi M et al (2012) Yeast-yeast interactions revealed by aromatic profile analysis of Sauvignon Blanc wine fermented by single or co-culture of non-Saccharomyces and Saccharomyces yeasts. Food Microbiol 32:243–253

    Article  CAS  Google Scholar 

  • Saerens SMG, Delvaux FR, Verstrepen KJ, Thevelein JM (2010) Production and biological function of volatile esters Saccharomyces cerevisiae. Microb Biotechnol 3:165–177

    Article  CAS  Google Scholar 

  • Saez JS, Lopes CA, Kirs VE, Sangorrín M (2011) Production of volatile phenols by Pichia manshurica and Pichia membranifaciens isolated from spoiled wines and cellar environment in Patagonia. Food Microbiol 28:503–509

    Article  CAS  Google Scholar 

  • Sáez JS, Lopes CA, Kirs VC, Sangorrín MP (2010) Enhanced volatile phenols in wine fermented with Saccharomyces cerevisiae and spoiled with Pichia guilliermondii and Dekkera bruxellensis. Lett Appl Microbiol 51:170–176

    Google Scholar 

  • Saikaly PE, Stroot PG, Oerther DB (2005) Use of 16S rRNA gene terminal restriction fragment analysis to assess the impact of solids retention time on the bacterial diversity of activated sludge. Appl Environ Microbiol 71:5814–5822

    Article  CAS  Google Scholar 

  • Schuller D, Cardoso F, Sousa S, Gomes P, Gomes AC, Santos MAS, Casal M (2012) Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions. PLoS ONE 7:e32507

    Article  CAS  Google Scholar 

  • Setati ME, Jacobson D, Andong U-C, Bauer F (2012) The vineyard yeast microbiome, a mixed model microbial map. PLoS ONE 7:e52609

    Article  CAS  Google Scholar 

  • Settanni L, Sannino C, Francesca N, Guarcello R, Moschetti G (2012) Yeast ecology of vineyards within Marsala wine area (western Sicily) in two consecutive vintages and selection of autochthonous Saccharomyces cerevisiae strains. J Biosci Bioeng 114:606–614

    Article  CAS  Google Scholar 

  • Sipiczki M (2006) Metschnikowia strains isolated from botrytized grapes antagonize fungal and bacterial growth by iron depletion. Appl Environ Microbiol 72:6716–6724

    Article  CAS  Google Scholar 

  • Stefanini I, Dapporto L, Legras J, Calabretta A, Di M (2012) Role of social wasps in Saccharomyces cerevisiae ecology and evolution. PNAS 109:13398–13403

    Article  CAS  Google Scholar 

  • Tello J, Cordero-Bueso G, Aporta I, Cabellos JM, Arroyo T (2012) Genetic diversity in commercial wineries: effects of the farming system and vinification management on wine yeasts. J Appl Microbiol 112:302–315

    Article  CAS  Google Scholar 

  • Tristezza M, Fantastico L, Vetrano C, Bleve G, Corallo D, Grieco F, Mita G (2014) Molecular and technological characterization of Saccharomyces cerevisiae strains isolated from natural fermentation of susumaniello grape must in Apulia, Southern Italy. Int J Microbiol 2014:1–12

    Article  Google Scholar 

  • Valero E, Cambon B, Schuller D, Casal M, Dequin S (2007a) Biodiversity of Saccharomyces yeast strains from grape berries of wine-producing areas using starter commercial yeasts. FEMS Yeast Res 7:317–329

    Article  CAS  Google Scholar 

  • Valero E, Cambon B, Schuller D, Casal M, Dequin S (2007b) Biodiversity of Saccharomyces yeast strains from grape berries of wine-producing areas using starter commercial yeasts. FEMS Yeast Res 7:317–329

    Article  CAS  Google Scholar 

  • Weiss S, Samson F, Navarro D, Casaregola S (2013) YeastIP: a database for identification and phylogeny of Saccharomycotina yeasts. FEMS Yeast Res 13:117–125

    Article  CAS  Google Scholar 

  • Yurkov AM, Kemler M, Begerow D (2011) Species accumulation curves and incidence-based species richness estimators to appraise the diversity of cultivable yeasts from beech forest soils. PLoS ONE 6:e23671

    Article  CAS  Google Scholar 

  • Yurkov AM, Kemler M, Begerow D (2012) Assessment of yeast diversity in soils under different management regimes. Fungal Ecol 5:24–35

    Article  Google Scholar 

  • Zott K, Claisse O, Lucas P, Coulon J, Lonvaud-Funel A, Masneuf-Pomarede I (2010) Characterization of the yeast ecosystem in grape must and wine using real-time PCR. Food Microbiol 27:559–567

    Article  CAS  Google Scholar 

  • Wang C, Liu Y (2013) Dynamic study of yeast species and Saccharomyces cerevisiae strains during the spontaneous fermentations of Muscat blanc in Jingyang, China. Food Microbiol 33(2):172–177

    Article  CAS  Google Scholar 

  • Zechmeister HR, Schmitzberger I, Steurer B, Peterseil J, Wrbka T (2003) The influence of land-use practices and economics on plant species richness in meadows. Biol Conserv 114(2):165–177

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to winery/vineyard owners for allowing us to collect the samples and financial support was provided by Epagri and Embrapa.

Author information

Authors and Affiliations

  1. Programa de Pós-graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    S. D. C. Mendes

  2. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    M. Ramírez-Castrillón & P. Valente

  3. Embrapa Produtos e Mercado, Escritório de Canoinhas, Canoinhas, SC, Brazil

    N. P. Feldberg

  4. Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Florianópolis, SC, Brazil

    S. D. C. Mendes & F. C. Bertoldi

  5. Research group in Mycology (GIM), Research center in Environmental Basic Sciences, Universidad Santiago de Cali, Calle 5 62-00, Cali, Valle Del Cauca, Colombia

    M. Ramírez-Castrillón

Authors
  1. S. D. C. Mendes
    View author publications

    Search author on:PubMed Google Scholar

  2. M. Ramírez-Castrillón
    View author publications

    Search author on:PubMed Google Scholar

  3. N. P. Feldberg
    View author publications

    Search author on:PubMed Google Scholar

  4. F. C. Bertoldi
    View author publications

    Search author on:PubMed Google Scholar

  5. P. Valente
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to S. D. C. Mendes.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

11274_2017_2298_MOESM1_ESM.docx

Supplementary Table1—Species richness and taxonomic diversity estimates obtained with different indices (mean and standard deviation). Abbreviations used: ACE, Abundance-based coverage estimator; ICE, incidence-based coverage estimator, Jackknife 2, second-order Jackknife richness estimator, Bootstrap, Shannon and reciprocal of Simpson (DOCX 13 KB)

11274_2017_2298_MOESM2_ESM.docx

Supplementary Table 2—Functional diversity in vineyards located at altitudes of 678, 973 and 1053 m obtained with the assimilation of different sources of nitrogen (mean and standard deviation). Abbreviations used: FAD2, Functional attribute diversity; FDc, community based; Rao Entropy. Significant differences are indicated by different lower case letters, p&#x003C;0.05 (DOCX 12 KB)

11274_2017_2298_MOESM3_ESM.docx

Supplementary Table 3—Functional diversity in vineyards located at altitudes of 678, 973 and 1053 m obtained with the assimilation of different sources of carbon (mean and standard deviation). Abbreviations used: FAD2, Functional attribute diversity; FDc, community based; Rao Entropy. Significant differences are indicated by different lower case letters, p&#x003C;0.05 (DOCX 12 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mendes, S.D.C., Ramírez-Castrillón, M., Feldberg, N.P. et al. Environmental yeast communities in vineyards in the mountains of Santa Catarina State, Brazil. World J Microbiol Biotechnol 33, 128 (2017). https://doi.org/10.1007/s11274-017-2298-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11274-017-2298-2

Keywords