Eukaryotic molecular diversity at different steps of the wastewater treatment plant process reveals more phylogenetic novel lineages
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Wastewater microbiota represents important actors of organic depollution. Nowadays, some species used as bioindicators of the effluent quality are still identified by microscopy. In the present study, we investigated eukaryotic diversity at the different steps of the treatment process of a wastewater treatment plant (aerobic, anaerobic, clarifier basins and anaerobic digester) using the 18S rRNA gene sequencing approach. Of the 1519 analysed sequences, we identified 160 operational taxonomic units. Interestingly, 56.9% of the phylotypes were assigned to novel phylogenetic molecular species since they show <97% sequence identity with their nearest affiliated representative within public databases. Peritrichia ciliates were the most predominant group, with Epistylis as the most common genus. Although anaerobic, the digester appears to harbor many unclassified phylotypes of protozoa species. Novel lineages such as LKM11 and LKM118 were widely represented in the digester. Diversity values given by Shannon indexes show that the clarifier is the most diversified. This work will help designing molecular tools that are fast, reliable, and reproducible for monitoring wastewater depollution and studying phylogenetic relationships among the wonderful world of protists within this anthropogenic ecosystem.
KeywordsActivated sludge Ciliates Cryptomycota LKM118 Wastewater microbiota 18S rRNA gene
We are very grateful to Susan Cure for reading the manuscript, the excellent technical assistance of the Genoscope sequencing team, Stephane Frenette for giving access to the Evry WWTP, M. Trouvé and D. Dehon for providing samples from the WWTP.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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