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Characterization and enzymatic hydrolysis of hydrothermally treated β-1,3–1,6-glucan from Aureobasidium pullulans

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Abstract

The chemical structure of hydrothermally treated β-1,3–1,6-glucan from Aureobasidium pullulans was characterized using techniques such as gas chromatography/mass spectrometry (GC/MS) and nuclear magnetic resonance (NMR). The chemical shifts of anomeric carbons observed in the 13C-NMR spectra suggested the presence of single flexible chains of polysaccharide in the sample. β-1,3–1,6-Glucan from A. pullulans became water-soluble, with an average molecular weight of 128,000 Da after hydrothermal treatment, and the solubility in water was approximately 10% (w/w). Sample (3% w/v) was completely hydrolyzed to glucose by enzymatic reaction with Lysing enzymes from Trichoderma harzianum. Gentiobiose (Glcβ1 → 6Glc) and glucose were released as products during the reaction, and the maximum yield of gentiobiose was approximately 70% (w/w). The molar ratio of gentiobiose to glucose after 1 h reaction suggested that the sample is likely highly branched. Sample (3% w/v) was also hydrolyzed to glucose by Uskizyme from Trichoderma sp., indicating that it is very sensitive to enzymatic hydrolysis.

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Authors and Affiliations

  1. Itochu Sugar Co. Ltd, 3 Tamatsuura, Hekinan-shi, Aichi, 447-8506, Japan

    Katsuki Hirabayashi & Nobuhiro Kondo

  2. The Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki-shi, Miyazaki, 889-2192, Japan

    Katsuki Hirabayashi & Sachio Hayashi

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  1. Katsuki Hirabayashi
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  2. Nobuhiro Kondo
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  3. Sachio Hayashi
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Correspondence to Sachio Hayashi.

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Hirabayashi, K., Kondo, N. & Hayashi, S. Characterization and enzymatic hydrolysis of hydrothermally treated β-1,3–1,6-glucan from Aureobasidium pullulans . World J Microbiol Biotechnol 32, 206 (2016). https://doi.org/10.1007/s11274-016-2167-4

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  • DOI: https://doi.org/10.1007/s11274-016-2167-4

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