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Diversity of the bacterial community in Myanmar traditional salted fish yegyo ngapi

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Abstract

The distribution and characterization of bacteria including lactic acid bacteria (LAB) in the traditional and popular salted fish yegyo ngapi in Myanmar were studied to clarify the contribution of these bacteria to the curing and ripening of this product. Samples of yegyo ngapi purchased from a market in Yangon were used. Most of the isolates obtained using de Man, Rogosa and Sharpe medium containing 10 % NaCl were identified as coccoid LAB on the basis of their basic phenotypic characteristics. From the results of 16S rRNA gene sequencing and PCR-restriction fragment length polymorphism analysis of this gene, most of the isolates were identified as the halophilic LAB Tetragenococcus muriaticus. Analyses of the 16S rRNA gene based on the clone library using DNA extracted from salted fish products were also performed. The results of these molecular-analysis-based techniques showed that spore-forming and non-spore-forming anaerobic bacteria including the genera Clostridium and Halanaerobium in addition to T. muriaticus were also frequently found in bacterial communities. These findings suggest that the anaerobic condition during curing and ripening resulted in bacterial communities composed of strictly anaerobic bacteria and halophilic LAB, and that these bacteria might also contribute to the manufacturing processes of this product. In addition, DNA sequences similar to that of Clostridium botulinum were found in the clone library analysis. Therefore, despite no reports of botulism poisoning from the region where the samples were taken, closer surveillance should be carried out from the viewpoint of food safety.

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Acknowledgments

The authors are extremely grateful to the Department of Fisheries, Ministry of Agriculture, Livestock and Irrigation for their assistance. This study was also supported by the Open Partnership Joint Projects of JSPS Bilateral Joint Research Projects.

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Authors and Affiliations

  1. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan

    Takeshi Kobayashi, Chihiro Taguchi, Kakeru Kida, Hiroko Matsuda, Takeshi Terahara & Chiaki Imada

  2. Department of Fisheries, Ministry of Agriculture, Livestock and Irrigation, Bayintnaung Road, West Gyo Gone, Insein Township, Yangon, Myanmar

    Nant Kay Thwe Moe & Su Myo Thwe

Authors
  1. Takeshi Kobayashi
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  2. Chihiro Taguchi
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  3. Kakeru Kida
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  4. Hiroko Matsuda
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  5. Takeshi Terahara
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  6. Chiaki Imada
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  7. Nant Kay Thwe Moe
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  8. Su Myo Thwe
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Correspondence to Takeshi Kobayashi.

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Kobayashi, T., Taguchi, C., Kida, K. et al. Diversity of the bacterial community in Myanmar traditional salted fish yegyo ngapi . World J Microbiol Biotechnol 32, 166 (2016). https://doi.org/10.1007/s11274-016-2127-z

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  • DOI: https://doi.org/10.1007/s11274-016-2127-z

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