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A Candida guilliermondii lysine hyperproducer capable of elevated citric acid production

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Abstract

A mutant of the yeast Candida guilliermondii ATCC 9058 exhibiting elevated citric acid production was isolated based upon its ability to overproduce lysine. This method involved the use of a solid medium containing a combination of lysine analogues to identify a mutant that produced a several-fold higher lysine level compared to its parent strain using glucose or glycerol as a carbon source. The mutant strain was also capable of producing more than a fivefold higher citric acid level on glycerol as a carbon source compared to its parent strain. It was concluded that the screening of yeast lysine hyperproducer strains could provide a rapid approach to isolate yeast citric acid hyperproducer strains.

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Acknowledgments

Financial support of this work was provided by the South Dakota Agricultural Experiment Station Grant SD00H434-12.

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  1. Thomas P. West

    Present address: Department of Chemistry, Texas A&M University-Commerce, Commerce, TX, 75429, USA

Authors and Affiliations

  1. Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA

    Thomas P. West

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  1. Thomas P. West
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Correspondence to Thomas P. West.

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West, T.P. A Candida guilliermondii lysine hyperproducer capable of elevated citric acid production. World J Microbiol Biotechnol 32, 73 (2016). https://doi.org/10.1007/s11274-016-2031-6

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  • DOI: https://doi.org/10.1007/s11274-016-2031-6

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