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Improving the yield of (+)-terrein from the salt-tolerant Aspergillus terreus PT06-2

World Journal of Microbiology and Biotechnology volume 32, Article number: 77 (2016) Cite this article

Abstracts

(+)-Terrein has a potential application for drug discovery. To improve the yield of (+)-terrein, two-level Plackett–Burman design and response surface methodology methods were used to optimize the condition of a salt-tolerant fungus, Aspergillus terreus PT06-2. As a result, the yield of (+)-terrein reached 8.20 ± 0.072 g/L in a 500-mL flask containing 150 mL optimal medium consisted of 13.1 % NaCl, 3.6 % starch, 2 % sodium glutamate, 0.05 % KCl, 3 % inoculum size, adjusting initial pH value to 5 with 10 % HCl and shaking for 18 days at 28 °C and 180 rpm. The production of (+)-terrein was 47.0 % higher than the highest production reported in shake flasks. The advantages of this optimization are uses of single carbon source and nitrogen source and easy separation and purification by recrystallization. The result exhibited the potential and advantages of A. terreus PT06-2 in industrial production of (+)-terrein by fermentation.

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Acknowledgments

This research was financially supported from NSFC (Nos. 81561148012, 41376148), from 863 programs of China (Nos. 2012AA092104, 2013AA092901), from NSFC-Guangdong Joint Fund Project (No. U1501221) and NSFC-Shandong Joint Fund for Marine Science Research Centers (No. U1406402), and from the Special Fund for Marine Scientific Research in the Public Interest of China (No. 201405038).

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    Authors and Affiliations

    1. Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China

      Chengying Zhao, Lei Guo, Guoliang Zhu & Weiming Zhu

    2. Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, 550002, China

      Liping Wang

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    1. Chengying Zhao
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    Correspondence to Weiming Zhu.

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    Chengying Zhao and Lei Guo have contributed equally to this work.

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    Zhao, C., Guo, L., Wang, L. et al. Improving the yield of (+)-terrein from the salt-tolerant Aspergillus terreus PT06-2. World J Microbiol Biotechnol 32, 77 (2016). https://doi.org/10.1007/s11274-016-2029-0

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    • DOI: https://doi.org/10.1007/s11274-016-2029-0

    Keywords

    • (+)-Terrein
    • Aspergillus terreus PT06-2
    • Fermentation optimization
    • Plackett–Burman design
    • Response surface methodology