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Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds

Abstract

In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Bacillus CBMDC3f was phylogenetically characterized as Bacillus subtilis subsp. subtilis after rRNA analysis of the 16S subunit and the gyrA gene (access codes Genbank JX120508 and JX120516, respectively). Its antibacterial potential was evaluated against Listeria monocytogenes (9 strains), B. cereus (3 strains) and Staphylococcus aureus ATCC29213. Its cell suspension and cell-free supernatant (CFS) exerted significant anti-Listeria and anti-S. aureus activities, while the lipopeptides fraction (LF) also showed anti-B. cereus effect. The UV-MALDI-MS analysis revealed surfactin, iturin and fengycin in the CFS, whereas surfactin predominated in the LF. The CFS from CBMDC3f contained surfactin, iturin and fengycin with four, two and four homologues per family, respectively, whereas four surfactin, one iturin and one fengycin homologues were identified in the LF. For some surfactin homologues, their UV-MALDI-TOF/TOF (MS/MS; Laser Induced Decomposition method, LID) spectra were also obtained. Mass spectrometry analysis contributed with relevant information about the type of lipopeptides that Bacillus strains can synthesize. From our results, surfactin would be the main metabolite responsible for the antibacterial effect.

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Acknowledgments

This work had the financial support of CIUNSa (PI 1974), UBA (0055BA) and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) of Argentina (PICT2011-07/67 and PICT 2012-0888) and CONICET-Argentina (PIP 0072CO and PIP 00019). M.C. Audisio, R. Erra-Balsells and G. Petroselli are Research Members of CONICET, Argentina.

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The authors declare not conflict of interest related to this work.

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Correspondence to M. C. Audisio.

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Torres, M.J., Petroselli, G., Daz, M. et al. Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds. World J Microbiol Biotechnol 31, 929–940 (2015). https://doi.org/10.1007/s11274-015-1847-9

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Keywords

  • Bacillus subtilis subsp. subtilis
  • Foodborne pathogens
  • Lipopeptide homologues
  • UV-MALDI-TOF MS
  • Surfactin