Abstract
The potential use of Sporosarcina pasteurii in possible biotechnological applications on a large scale (ground improvement, consolidation of building structures and ornamental stone, or in developing bio-materials for the building industry), is based on its ability to produce high amounts of carbonate in a short period of time via urea hydrolysis. Industrial biomass production would have a low environmental impact and would be most economical if the standard growth media could be replaced with alternative nutrient sources, such as byproducts or wastes from other industries, or other low cost ingredients. The use of cost effective ingredients must guarantee ureolytic activities and growth conditions that are comparable to those resulting from the standard nutrient medium. In this work, three types of alternative media were tested for growing the ureolytic active bacteria S. pasteurii: (1) alternative nutrient sources such as industrial wastes resulting from the dairy and brewery industries, (2) fertilizer urea as an alternative urea substitute, and (3) different types of poultry manure based fertilizers as nutrient and urea substitutes. The comparison between the standard media, the nutrient alternatives and urea substitutes was possible by taking the protein concentration and nitrogen content into account. Bacterial activity was evaluated in terms of biomass changes over time (CFU, optical density, ATP measurements) and indirect estimation of the enzyme production (Nessler assay, conductivity measurement). The results revealed that some of the dairy wastes tested, such as whey and buttermilk, are potential alternative nutrients for bacterial development, while the urea fertilizer is perfectly suitable as an economical substitute for pure laboratory grade urea.
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References
Achal V, Pan X (2011) Characterization of urease and carbonic anhydrase producing bacteria and their role in calcite precipitation. Curr Microbiol 62:894–902. doi:10.1007/s00284-010-9801-4
Achal V, Mukherjee A, Basau PC, Reddy MS (2009) Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii. J Ind Microbiol Biotechnol 36:433–438. doi:10.1007/s10295-008-0514-7
Al-Thawadi SM (2008) High strength in situ biocementation of soil by calcite precipitating locally isolated ureolytic bacteria. PhD Dissertation. Murdoch University Australia
Burbank MB, Weaver TJ, Green TL, Williams BC, Crawford RL (2011) Precipitation of calcite by indigenous microorganisms to strengthen liquefiable soils. Geomicrobiol J 28:301–312. doi:10.1080/01490451.2010.499929
Chahal N, Rajor A, Siddique R (2011) Calcium carbonate precipitation by different bacterial strains. Afr J Biotechnol 10:8359–8372. doi:10.5897/AJB11.345
Chunxiang Q, Jianyun W, Ruixing W, Liang C (2009) Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii. Mater Sci Eng C 29:1273–1280. doi:10.1016/j.msec.2008.10.025
de Muynck W, Debrouwer D, de Belie N, Verstraete W (2008) Bacterial carbonate precipitation improves the durability of cementitious materials. Cem Concr Res 38:1005–1014. doi:10.1016/j.cemconres.2008.03.005
Fujita Y, Ferris FG, Lawson RD, Colwell FS, Smith RW (2000) Calcium carbonate precipitation by ureolytic subsurface bacteria. Geomicrobiol J 17:305–318. doi:10.1080/01490450050193360
Fujita Y, Taylor JL, Gresham TLT, Delwiche ME, Colwell FS, McLing TL, Petzke LM, Smith RW (2008) Stimulation of microbial urea hydrolysis in groundwater to enhance calcite precipitation. Environ Sci Technol 42:3025–3032. doi:10.1021/es702643g
Hammad IA, Talkhan FN, Zoheir AE (2013) Urease activity and induction of calcium carbonate precipitation by Sporosarcina pasteurii NCIMB 8841. J Appl Sci Res 9:1525–1533. ISSN 1819-544X
Hammes F, Boon N, de Villiers J, Verstraete W, Siciliano SD (2003) Strain-specific ureolytic microbial calcium carbonate precipitation. Appl Environ Microbiol 69:4901–4909. doi:10.1128/AEM.69.8.4901-4909.2003
Kim HK, Park SJ, Han JI, Lee HK (2013) Microbially mediated calcium carbonate precipitation on normal and lightweight concrete. Constr Build Mater 38:1073–1082. doi:10.1016/j.conbuildmat.2012.07.040
Li W, Liu L-P, Zhou P-P, Cao L, Yu L-J, Jiang S-Y (2011) Calcite precipitation induced by bacteria and bacterially produced carbonic anhydrase. Curr Sci 100:502–508
Mitchell AC, Dideriksen K, Spangler LH, Cunningham AB, Gerlach R (2010) Microbially enhanced carbon capture and storage by mineral-trapping and solubility-trapping. Environ Sci Technol 44:5270–5276. doi:10.1021/es903270w
Parks SL (2009) Kinetics of calcite precipitation by ureolytic bacteria under aerobic and anaerobic conditions. Master thesis. Montana State University, USA
Stocks-Fisher S, Galiant GK, Bang SS (1999) Microbiological precipitation of CaCO3. Soil Biol Biochem 31:1563–1571. doi: 10.1016/S0038-0717(99)00082-6
Tobler DJ, Cuthbert MO, Greswell RB, Riley MS, Renshaw JC, Handley-Sidhu S, Phoenix VR (2011) Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite. Geochim Cosmochim Acta 75:3290–3301. doi:10.1016/j.gca.2011.03.023
Whiffin VS (2004) Microbial CaCO3 precipitation for the production of biocement. PhD Dissertation. Murdoch University Australia
Whiffin VS, van Paassen LA, Harkes MP (2007) Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol J 24:417–423. doi:10.1080/01490450701436505
Acknowledgments
This work is made under the framework of the ECO-CEMENT project (FP7- Grant 282922). The authors would like to thank Giuliano Tazzari (Amek srl, Italy), Gianfranco Betti (Mukki Latte, Italy), Mr. Roberto (I Marzoli, Italy), Juergen Harves (a farm in Oyten, Germany) and Javier Arroyo Abancéns (Solintel) for their kindness in supplying the alternative sources to test, to Jay Stuart (DWEcoCo) for his precious assistance in improving the paper and to Laura Sánchez Alonso (Essentium) for the exemplary coordination of the project.
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Cuzman, O.A., Richter, K., Wittig, L. et al. Alternative nutrient sources for biotechnological use of Sporosarcina pasteurii . World J Microbiol Biotechnol 31, 897–906 (2015). https://doi.org/10.1007/s11274-015-1844-z
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DOI: https://doi.org/10.1007/s11274-015-1844-z