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Potential probiotic attributes of a new strain of Bacillus coagulans CGMCC 9951 isolated from healthy piglet feces

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Abstract

A new strain of Bacillus coagulans CGMCC 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including Escherichia coli O8, Staphylococcus aureus, Salmonella enterica subsp. enterica serovar enteritidis, Streptococcus suis, Listeria monocytogenes and Pasteurella multocida, was isolated from healthy piglet feces. In adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of B. subtilis JT143 and L. acidophilus LY24 respectively isolated from BioPlus®2B and FloraFIT® Probiotics (P < 0.05). The adhesion activity reached 44.5 ± 3.2, 48.9 ± 2.6, 42.6 ± 3.3 and 37.6 ± 2.4 % to jejunum, ileum, transverse colon and sigmoid colon, separately. The survival rate of B. coagulans CGMCC 9551 was reduced by only 20 % at 4 h exposure under 0.9 % w/v bile salt. The strain was fully resistant to pH 2 for 2 h with 90.1 ± 3.5 % survival and susceptible to 15 antibiotics commonly used in veterinary medicine. Additionally, the bacteria showed amylase, protease and cellulase activities. The safety assessment demonstrated the lack of toxicity potential in B. coagulans CGMCC 9551 by ligated rabbit ileal loop assay, acute and subchronic toxicity test. These results implied that that the new strain of B. coagulans CGMCC 9951 isolated from healthy piglet feces has promising probiotic characteristics and offers desirable opportunities for its successful commercialization as one excellent candidate probiotic.

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Acknowledgments

We are grateful to Dr. Ke Ding for generous gifts of pathogenic bacteria and Dr. Bin Zhang for helpful discussions and critical reading of the manuscript. This work was supported by The Key Scientific and Technological Project of Henan Province under Grant No. 122102110034 and National University Student Innovation Training Program No. 111416090109.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical strandard

The present study was conducted in accordance with the principles outlined in the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals (http://grants1.nih.gov/grants/olaw/) was approved by the local animal ethics committee at Henan University of Science and Technology (HUST). All procedures performed in studies involving animals were in accordance with the ethical standards of the local animal ethics committee at HUST.

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Informed consent was obtained from all individual participants included in the study.

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Authors and Affiliations

  1. College of Food and Bioengineering, Henan University of Science and Technology, No. 263, Kaiyuan Ave., 471023, Luoyang, People’s Republic of China

    Shao-Bin Gu, Li-Na Zhao, Ying Wu, Shi-Chang Li, Jian-Rui Sun, Jing-Fang Huang & Dan-Dan Li

  2. Hefei Institutes of Physical Science, Chinese Academy of Sciences, 230031, Hefei, People’s Republic of China

    Ying Wu

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  1. Shao-Bin Gu
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Correspondence to Shao-Bin Gu.

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Gu, SB., Zhao, LN., Wu, Y. et al. Potential probiotic attributes of a new strain of Bacillus coagulans CGMCC 9951 isolated from healthy piglet feces. World J Microbiol Biotechnol 31, 851–863 (2015). https://doi.org/10.1007/s11274-015-1838-x

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