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Asparagopsis armata and Sphaerococcus coronopifolius as a natural source of antimicrobial compounds

Abstract

Methanol, n-hexane and dichloromethane extracts of twelve marine macro-algae (Rhodophyta, Chlorophyta and Heterokontophyta divisions) from Peniche coast (Portugal) were evaluated for their antibacterial and antifungal activity. The antibacterial activity was evaluated by disc diffusion method against Bacillus subtilis (gram positive bacteria) and Escherichia coli (gram negative bacteria). Saccharomyces cerevisiae was used as a model for the antifungal activity by evaluating the growth inhibitory activity of the extracts. The high antibacterial activity was obtained by the Asparagopsis armata methanolic extract (10 mm–0.1 mg/disc), followed by the Sphaerococcus coronopifolius n-hexane extract (8 mm–0.1 mg/disc), and the Asparagopsis armata dichloromethane extract (12 mm–0.3 mg/disc) against Bacillus subtilis. There were no positive results against Escherichia coli. Sphaerococcus coronopifolius revealed high antifungal potential for n-hexane (IC50 = 40.2 µg/ml), dichloromethane (IC50 = 78.9 µg/ml) and methanolic (IC50 = 55.18 µg/ml) extracts against Saccharomyces cerevisiae growth. The antifungal potency of the Sphaerococcus coronopifolius extracts was similar with the standard amphotericin B. Asparagopsis armata and Sphaerococcus coronopifolius reveal to be interesting sources of natural compounds with antimicrobial properties.

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Acknowledgments

This work was funded by FP7 EU project “BAMMBO: Sustainable production of Biologically Active Molecules of Marine Based Origin” (FP7 No. 265896/http://www.bammbo.eu).

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Correspondence to Rui Pedrosa.

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Pinteus, S., Alves, C., Monteiro, H. et al. Asparagopsis armata and Sphaerococcus coronopifolius as a natural source of antimicrobial compounds. World J Microbiol Biotechnol 31, 445–451 (2015). https://doi.org/10.1007/s11274-015-1797-2

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Keywords

  • Marine algae
  • Antibacterial activity
  • Antifungal activity
  • Seaweeds
  • Marine natural compounds
  • Natural antibiotics