World Journal of Microbiology and Biotechnology

, Volume 31, Issue 2, pp 399–406 | Cite as

Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

  • J. C. Lapenda
  • P. A. Silva
  • M. C. Vicalvi
  • K. X. F. R. Sena
  • S. C. Nascimento
Original Paper


Prodigiosin is an alkaloid and natural red pigment produced by Serratia marcescens. Prodigiosin has antimicrobial, antimalarial and antitumor properties and induces apoptosis in T and B lymphocytes. These properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries. The aim of the present study was to evaluate the antimicrobial activity of prodigiosin against pathogenic micro-organisms. The red pigments produced by S. marcescens exhibited absorption at 534 nm, Rf of 0.59 and molecular weight of 323 m/z. Antimicrobial activity was tested against oxacillin-resistant Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes, Acinetobacter sp. and oxacillin-resistant S. aureus. The standard antibiotics employed were ampicillin, chloramphenicol, gentamicin and oxacillin. The disc-diffusion tests demonstrated significant inhibition zones for S. aureus (35 ± 0.6), E. faecalis (22 ± 1.0) and S. pyogenes (14 ± 0.6). However, prodigiosin showed resistance to E. coli, P. aeruginosa and acinetobacter, where no significant formation of inhibitory halos were observed. We determined the inhibitory minimum concentrations and bactericidal for 20 strains of oxacillin-resistant S. aureus (ORSA). The pattern was the antibiotic oxacillin. The minimum inhibitory concentrations observed ranged from 1, 2 and 4.0 μg/mL, respectively, while the minimum bactericidal concentrations ranged from 2, 4, 8 and 16 μg/mL. The S. marcescens prodigiosin produced by showed bactericidal and bacteriostatic effect showing promising antimicrobial activity and suggesting future studies regarding its applicability in antibiotics therapies directed ORSA.


Antibiotics Serratia marcescens Resistance Natural products 



The authors are grateful to the technical team, the Department of Antibiotics of the Federal University of Pernambuco for offering the use of the laboratories and to the Brazilian fostering agencies CNPq and CAPES.


  1. Abdullateef AA, Ahmad AAR, Hani AAS, Jalal AB, Mohammad IA, Ghada BS (2012) Documentation of vancomycin-resistant Staphylococcus aureus (VRSA) among children with atopic dermatitis in the Qassim region, Saudi Arabia. Acta Dermatovenerol 21:51–53Google Scholar
  2. Baldino C, Parr J, Wilson C, Yohannes D, Wasserman H (2006) Indoloprodigiosins from the C-10 bipyrrolic precursor: new antiproliferative prodigiosin analogs. Bioorg Med Chem Lett 16:701–704CrossRefGoogle Scholar
  3. Bauer AW, Kirby WMM, Sherris JC et al (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496Google Scholar
  4. Blake AJ, Hunter GA, McNab H (1990) A short synthesis of prodigiosin analogues. J Chem Soc Chem Commun 10:734–736CrossRefGoogle Scholar
  5. Chaves MH (1997) Análise de extratos de plantas por CCD: uma metodologia aplicada à disciplina “Química Orgância”. Quim Nova 20(5):560–562CrossRefGoogle Scholar
  6. Clinical and Laboratory Standards Institute (2010) Document: M100-S20 Vol. 30 No. 1. Replaces M100-S19 Vol. 19:3Google Scholar
  7. Coelho SMO, Moraes RAM, Soares LC, Pereira IA, Gomes LP, Souza MMS (2013) Resistance pattern and detection of mecA gene in oxacillin-resistant isolates of Staphylococcus aureus and Staphylococcus intermedius from animal and human samples. Ciência Rural 37:195–200CrossRefGoogle Scholar
  8. Croes S, Beisser PS, Terporten PH, Neef C, Deurenberg RH, Stobberingh EE (2009) Diminished in vitro antibacterial activity of oxacillin against clinical isolates of borderline oxacillin-resistant Staphylococcus aureus. Clin Microbiol Infect 16:979–985CrossRefGoogle Scholar
  9. Davaraj N, Dharumaduari D, Noorudin T, Annamalai P (2009) Production of prodigiosin from Serratia marcescens and its cytotoxicity activity. J Pharm Res 2:590–593Google Scholar
  10. El-Bondkly AM, El-Gendy MM, Bassyouni RH (2012) Overproduction and biological activity of prodigiosin-like pigments from recombinant fusant of endophytic marine Streptomyces species. Antonie Van Leeuwenhoek 102:719–734Google Scholar
  11. Giri A, Anandkumar G, Muthukumaran G, Pennathur G (2004) A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol 4:11CrossRefGoogle Scholar
  12. Hemaiswarya S, Kruthiventi A, Doble M (2008) Synergism between natural products and antibiotics against infectious diseases. Phytomedicine 15:639–652CrossRefGoogle Scholar
  13. Jeong H, Yim H, Lee C, Choi H, Park Y, Yoon S, Hur G, Kang H, Kim D, Lee H, Park K, Park S, Lee K, Kim J (2005) Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent. Nucleic Acids Res 33:7066–7073CrossRefGoogle Scholar
  14. Lee JS, Kim YS, Park S, Kim J, Kang S, Lee M, Ryu S, Choi JM, Oh TK, Yoo JH (2011) Exceptional production of both prodigiosin and cycloprodigiosin as major metabolic constituents by a novel marine bacterium, Zooshikella rubidus S1-1. Appl Environ Microbiol 77:4967CrossRefGoogle Scholar
  15. Marcelo JM, Rozane LC, Eitan NB, Neiva D, Hélio HCF (2012) Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients. Rev Inst Med Trop 54:305–306CrossRefGoogle Scholar
  16. Nakashima T, Kurachi M, Kato Y, Yamaguchi K, Oda T (2005) Characterization of bacterium isolated from the sedimenta t Coastal área of Omura Bay in Japan and several biological activities of pigment produced by this isolated. Microbiol Immunol 49:407–415CrossRefGoogle Scholar
  17. Nakashima T, Myiazaki Y, Matsuyam Y, Muraoka W, Yamaguchi K, Oda T (2006) Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium γ-proteobacterium. Appl Microbiol Biotechnol 73:684–690CrossRefGoogle Scholar
  18. Packiavathy IASV, Priya S, Pandian SK, Ravi AV (2014) Inhibition of biofilm development of uropathogens by curcuminan anti-quorum sensing agent from Curcuma longa. Food Chem 148:453–460CrossRefGoogle Scholar
  19. Priya KA et al (2013) Antifouling activity of prodigiosin from estuarine isolate of Serratia marcescens CMST 07. In: Microbiological research in agroecosystem management. Springer, India, pp 11–21Google Scholar
  20. Santos AL, Dilvani OS, Cícero CF, Bruno LAF, Ilídio FA, Carlos RR, Helena CC (2011) Staphylococcus aureus: visitando uma cepa de importância hospitalar. J Bras Patol Med Lab 43(6):413–423CrossRefGoogle Scholar
  21. Silva RK, Subha DB, Ghosh AR, Babu S (2012) Characterization and enhanced production of prodigiosin from the spoiled coconut. Appl Biochem Biotechnol 166:187–196CrossRefGoogle Scholar
  22. Vendruscolor F, Fernanda K, de Luana OP, Jorge LN (2007) Production of single cell protein from apple pomace using state solid fermentation. Revista Brasileira de Tecnologi Agroindustrial 01:53–57Google Scholar
  23. Yang F, Wei HY, Li XQ, Li YH, Li XB, Yin LH, Pu YP (2013) Isolation and characterization of an algicidal bacterium indigenous to Lake Taihu with a red pigment able to lyse Microcystis aeruginosa. Biomed Environ Sci 26(2):148–154Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • J. C. Lapenda
    • 1
  • P. A. Silva
    • 2
  • M. C. Vicalvi
    • 3
  • K. X. F. R. Sena
    • 4
  • S. C. Nascimento
    • 4
  1. 1.Federal University of PernambucoRecifeBrazil
  2. 2.Licensing in ChemistryRural Federal University of PernambucoRecifeBrazil
  3. 3.Rural Federal University of PernambucoRecifeBrazil
  4. 4.Department of AntibioticsFederal University of PernambucoRecifeBrazil

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