Skip to main content

Advertisement

SpringerLink
Log in

A monoclonal antibody against 47.2 kDa cell surface antigen prevents adherence and affects biofilm formation of Candida albicans

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Candida albicans is an opportunistic dimorphic pathogen that exists in both planktonic and biofilm phases causing deep-rooted infections in mainly immunocompromised patients. Antibodies are believed to play anti-Candida activity by different mechanisms, like inhibition of adhesion and neutralization of virulence-related antigens. Inhibition of adhesion is one of the important strategies to prevent Candida infections and biofilm formation. In this study, monoclonal antibody (MAb 7D7) against C. albicans biofilm cell surface antigen (47.2 kDa) was generated to determine the changes in adherence and viability of C. albicans. In this regard XTT assay was carried out in 30, 60, 90 min and 48 h (maturation time) time points using MAb 7D7 and it (MAb 7D7) was found to be effective against adhesion and the formation of C. albicans biofilm on polystyrene as well as monolayer of human epithelial cells (HeLa). This result may also prove to be a valuable addition to the reagents available to study C. albicans cell surface dynamics and interaction of the fungus with host cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Bakshi D, Jain R, Tuteja U, Batra HV (2007) Generation and characterization of a lipopolysaccharide-specific murine monoclonal antibody to Proteus vulgaris OX19. World J Microbiol Biotechnol 23:817–821

    Article  CAS  Google Scholar 

  • Bugli F, Cacaci M, Martini C, Torelli R et al (2013) Human monoclonal antibody based therapy in the treatment of invasive candidiasis. Clin Dev Immunol 403121:9

    Google Scholar 

  • Bujdakova H, Paulovicova E, Borecka Melkusova S, Gasperık J et al (2008) Antibody response to the 45 kDa Candida albicans antigen in an animal model and potential role of the antigen in adherence. J Med Microbiol 57:1466–1472

    Article  CAS  Google Scholar 

  • Bujdakova H, Paulovicova E, Paulovicova L, Simova Z (2010) Participation of the Candida albicans surface antigen in adhesion, the first phase of biofilm development. FEMS Immunol Med Microbiol 59:485–492

    CAS  Google Scholar 

  • Cateau E, Levasseur P, Borgonovi M, Imbert C (2007) The effect of aminocandin (HMR 3270) on the in vitro adherence of Candida albicans to polystyrene surfaces coated with extracellular matrix proteins or fibronectin. Clin Microbiol Infect 13:311–315

    Article  CAS  Google Scholar 

  • Chaffin WL (2008) Candida albicans cell wall proteins. Microbiol Mol Biol Rev 72:495–544

    Article  CAS  Google Scholar 

  • Chaturvedi KA, Kavishwar A, Shiva Keshava GB, Shukla PK (2005) Monoclonal Immunoglobulin G1 Directed against Aspergillus fumigatus Cell Wall Glycoprotein Protects against Experimental Murine Aspergillosis. Clin Diagn Lab Immunol 12:1063–1068

    CAS  Google Scholar 

  • Franklyn KM, Warmington JR, Ott AK, Ashman RB (1990) An immunodominant antigen of Candida alhicans shows homology to the enzyme enolase. Immunol Cell Biol 68:173–178

    Article  CAS  Google Scholar 

  • Harriott MM, Noverr MC (2011) Importance of Candida bacterial polymicrobial biofilms in disease. Trends Microbiol 19:557–563

    Article  CAS  Google Scholar 

  • Huynh ML, Russell P, Walsh B (2009) Tryptic digestion of in-gel proteins for mass spectrometry analysis. Methods Mol Biol 519:507–513

    Article  CAS  Google Scholar 

  • Francolini I, Donelli G (2010) Prevention and control of biofilm-based medical-device- related infections. FEMS Immunol Med Microbiol 59:227–238

    CAS  Google Scholar 

  • Jarvis WR (1995) Epidemiology of nosocomial fungal infections, with emphasis on Candida species. Clin Infect Dis 20:1526–1530

    Article  CAS  Google Scholar 

  • Kumamoto CA (2011) Inflammation and gastrointestinal Candida colonization. Curr Opin Microbiol 14:386–391

    Article  CAS  Google Scholar 

  • Kumar M, Shukla PK (2006) Single-stranded conformation polymorphism of large subunit of ribosomal RNA is best suited to diagnosing fungal infections and differentiating fungi at species level. Diagn Microbiol Infect Dis 56:45–51

    Article  CAS  Google Scholar 

  • Li X, Yan Z, Xu J (2003) Quantitative variation of biofilms among strains in natural populations of Candida albicans. Microbiology 149:353–362

    Article  CAS  Google Scholar 

  • Li F-Q, Ma C-F, Shi L-N, Lu J-F, Wang Y, Huang M, Kong Q-Q (2013) Diagnostic value of immunoglobulin G antibodies against Candida enolase and fructose-bisphosphate aldolase for candidemia. BMC Infect Dis 13:253–261

    Article  CAS  Google Scholar 

  • Lopez-Villar E, Monteoliva L, Larsen MR, Sachon E et al (2006) Genetic and proteomic evidences support the localization of yeast enolase in the cell surface. Proteomics 6(Suppl 1):S107–S118

    Article  Google Scholar 

  • Macfarlane S, Dillon JF (2007) Microbial biofilms in the human gastrointestinal tract. J Appl Microbiol 102:1187–1196

    Article  CAS  Google Scholar 

  • Maza EN, Moraques MD, Pont´on J (2009) Monoclonal antibody-mediated inhibition of adhesion of Candida albicans and Candida dubliniensis to human epithelial cells. Eur J Oral Sci 117:474–478

    Article  Google Scholar 

  • Masaki M, Sato T, Sugawara Y, Sasano T et al (2011) Detection and identification of non-Candida albicans species in human oral lichen planus. Microbiol Immunol 55:66–70

    Article  CAS  Google Scholar 

  • Pietrella D, Bistoni G, Corbucci C, Perito S et al (2006) Candida albicans mannoprotein influences the biological function of dendritic cells. Cell Microbiol 8:602–612

    Article  CAS  Google Scholar 

  • Pitarch A, Abian J, Carrascal M, Sanchez M et al (2004) Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies. Proteomics 4:3084–3106

    Article  CAS  Google Scholar 

  • Pitarch A, Jimenez A, Nombela C, Gil C (2006) Decoding serological response to Candida cell wall immunome into novel diagnostic, prognostic, and therapeutic candidates for systemic candidiasis by proteomic and bioinformatic analyses. Mol Cell Proteomics 5:79–96

    Article  CAS  Google Scholar 

  • Potera C (1999) Microbiology forging a link between biofilms and disease. Science 283:1837–1839

    Article  CAS  Google Scholar 

  • Ramage G, Mowat E, Jones B, Williams C et al (2009) Our current understanding of fungal biofilms. Crit Rev Microbiol 35:340–355

    Article  CAS  Google Scholar 

  • Richards MJ, Edwards JR, Culver DH, Gaynes RP (1999) Nosocomial infections in medical intensive care units in the United States. National nosocomial infections surveillance system. Crit Care Med 27:887–892

    Article  CAS  Google Scholar 

  • Rodier MH, Imbert C, Kauffmann-Lacroix C, Daniault G et al (2003) Immunoglobulins G could prevent adherence of Candida albicans to polystyrene and extracellular matrix components. J Med Microbiol 52:373–377

    Article  CAS  Google Scholar 

  • Romeo O, De Leo F, Criseo G (2009) Adherence ability of Candida africana: a comparative study with Candida albicans and Candida dubliniensis. Mycoses 54:e57–e61

    Article  Google Scholar 

  • Samaranayake LP, MacFarlane TW (1981) The adhesion of the yeast Candida albicans to epithelial cells of human origin in vitro. Arch Oral Biol 26:815–820

    Article  CAS  Google Scholar 

  • Samaranayake LP, MacFarlane TW (1982) The effect of dietary carbohydrates on the in vitro adhesion of Candida albicans to epithelial cells. J Med Microbiol 15:511–517

    Article  CAS  Google Scholar 

  • San Millan R, Ezkurra PA, Quindbs G, Robert R et al (1996) Effect of monoclonal antibodies directed against Candida albicans cell wall antigens on the adhesion of the fungus to polystyrene. Microbiology 142:2271–2277

    Article  CAS  Google Scholar 

  • San Millan R, Elguezabal N, Regulez P, Moragues MD et al (2000) Effect of salivary secretory IgA on the adhesion of Candida albicans to polystyrene. Microbiology 146:2105–2112

    Google Scholar 

  • Sandini S, Melchionna R, Arancia S, Gomez MJ, La Valle R (1999) Generation of a highly immunogenic recombinant enolase of the human opportunistic pathogen Candida albicans. Biotechnol Appl Biochem 29:223–227

    CAS  Google Scholar 

  • Sentandreu M, Elorza MV, Sentandreu R, Gozalbo D (1995) Cloning of cDNAs coding for Candida albicans cell surface proteins. J Med Vet Mycol 33:105–111

    Article  CAS  Google Scholar 

  • Umazume M, Ueta E, Osaki T (1995) Reduced inhibition of Candida albicans adhesion by saliva from patients receiving oral cancer therapy. J Clin Microbiol 33:432–439

    CAS  Google Scholar 

  • Videyappan G, Bikandi J, Braley R, Chaffin WL (2000) Cell surface proteins of Candida albicans: preparation of extracts and improved detection of proteins. Electrophoresis 21:956–961

    Article  Google Scholar 

  • Walsh TJ, Hathorn JW, Sobel JD et al (1991) Detection of circulating Candida enolase by immunoassay in patients with cancer and invasive candidiasis. N Engl J Med 324:1545–1551

    Article  Google Scholar 

  • Zijnge V, Van Leeuwen MB, Degener JE, Abbas F et al (2010) Oral biofilm architecture on natural teeth. PLoS ONE 5:e9321

    Article  Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Director CSIR CDRI, Lucknow India for providing facilities. NNM thanks ICMR, New Delhi for a Senior Research Fellowship. CDRI Communication No. 8819.

Conflict of interest

The authors declare to have no conflict of interest.

Author information

Authors and Affiliations

  1. Medical Mycology Lab, Division of Microbiology, CSIR-Central Drug Research Institute, Sitapur Road, Lucknow, 226 031, India

    Nripendra Nath Mishra & Praveen K. Shukla

  2. Department of Biochemistry, Jamia Hamdard, New Delhi, India

    Shakir Ali

Authors
  1. Nripendra Nath Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shakir Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Praveen K. Shukla
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Praveen K. Shukla.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mishra, N.N., Ali, S. & Shukla, P.K. A monoclonal antibody against 47.2 kDa cell surface antigen prevents adherence and affects biofilm formation of Candida albicans . World J Microbiol Biotechnol 31, 11–21 (2015). https://doi.org/10.1007/s11274-014-1760-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-014-1760-7

Keywords

Search

Navigation