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Effect of earlier unfolded protein response and efficient protein disposal system on cellulase production in Rut C30

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Abstract

Trichoderma reesei (T. reesei) has been widely used in production of cellulolytic enzymes and heterologous proteins because of its high secretion capacity. The lack of knowledge on protein secretion mechanisms, however, still hinders rational improvement on cellulase production. The transcript levels of cellulases and components involved in post-transcriptional procedures were compared in this study between two mutants, QM9414 and Rut C30 for evaluating the effects of modification and secretion upon cellulase production. The results showed that cellulase induction by cellulose drastically up-regulated expressions of the sensor of unfolded protein, chaperone and folding-assisted enzymes in endoplasmic reticulum and resulted in unfolded protein response (UPR) and low-grade increase in secretory transporters’ expression similar to that of chemical treatment. Rut C30 demonstrated earlier and more sustainable expressions of elements involved in UPR and lower amount of cellular retained cellulase compared to QM9414, indicating that Rut C30 had hypercellulolytic property partially for its earlier and enhanced UPR to more efficiently dispose of protein. Modifying post-translational peptides and enhancing protein flux to avoid protein accumulation during cellulase production may be a feasible approach for strain improvement.

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Abbreviations

ERAD:

Endoplasmic reticulum associated degradation

FPA:

Filter paper activity

PDA:

Potato dextrose agar

RESS:

Repression under secretion stress

UPR:

Unfolded protein response

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Acknowledgments

This research was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA023202, 2012AA101807) and National Natural Science Foundation of China (21106102).

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Authors and Affiliations

  1. Tianjin Key Laboratory of Industrial Biosystem and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People’s Republic of China

    Guokun Wang, Dongyuan Zhang & Shulin Chen

  2. University of Chinese Academy Sciences, 19A, Yuquan Road, Beijing, 100049, China

    Guokun Wang

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  1. Guokun Wang
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Correspondence to Shulin Chen.

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Wang, G., Zhang, D. & Chen, S. Effect of earlier unfolded protein response and efficient protein disposal system on cellulase production in Rut C30. World J Microbiol Biotechnol 30, 2587–2595 (2014). https://doi.org/10.1007/s11274-014-1682-4

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