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Expression of bioactive porcine interferon-alpha in Lactobacillus casei

World Journal of Microbiology and Biotechnology volume 30, pages 2379–2386 (2014)Cite this article

Abstract

In this study, we constructed an expression cassette containing the inducible lac promoter and the secretion signal from an S-layer protein of Lactobacillus brevis for the expression of porcine interferon-alpha (IFN-α) in Lactobacillus casei (Lb. casei). Reverse-transcriptase PCR verified the presence of porcine IFN-α mRNA in the recombinant Lb. casei. The porcine IFN-α protein expressed in the recombinant Lb. casei was identified by both Western blot analysis and ELISA. We used various pH values and induction times to optimize the yield of IFN-α, and found that induction with 0.8 % lactose for 16 h under anaerobic conditions produced the highest concentrations of IFN-α. Furthermore, the activity of porcine IFN-α in the cultural supernatant was evaluated on ST cells infected with pseudorabies virus. The results revealed that porcine IFN-α inhibited virus replication in vitro. The findings of our study indicate that recombinant Lb. casei producing porcine IFN-α has great potential for use as a novel oral antiviral agent in animal healthcare.

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Acknowledgments

This work was funded by the Important Henan Province Science & Technology Specific Projects (111100110300), and the Innovation Scientists and Technicians Troop Construction Projects of Zhengzhou City (10CXTD148).

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Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Nongye Road 63#, Zhengzhou, 450002, Henan Province, People’s Republic of China

    Shi-jie Ma, Xin-Sheng Li, Xiao-Qing Guo, Peng-Fei Fu, Ming-Fan Yang & Hong-Ying Chen

  2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, 730046, China

    Kun Li

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  1. Shi-jie Ma
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  2. Kun Li
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  3. Xin-Sheng Li
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  7. Hong-Ying Chen
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Correspondence to Xin-Sheng Li or Hong-Ying Chen.

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Shi-jie Ma and Kun Li have contributed equally to this work.

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Ma, Sj., Li, K., Li, XS. et al. Expression of bioactive porcine interferon-alpha in Lactobacillus casei . World J Microbiol Biotechnol 30, 2379–2386 (2014). https://doi.org/10.1007/s11274-014-1663-7

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  • DOI: https://doi.org/10.1007/s11274-014-1663-7

Keywords

  • Porcine interferon-alpha
  • Lactobacillus casei
  • pIAbeta8
  • Oral antiviral agent
  • Pseudorabies virus