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Production and molecular characterization of somatic hybrids between Pleurotus florida and Lentinula edodes

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Abstract

Nine inter-generic somatic hybrids named as pfle were produced through PEG-mediated protoplast fusion between Pleurotus florida and Lentinula edodes using double selection method. Hybridity of the newly developed strains was established on the basis of colony morphology, mycelial growth, hyphal traits, fruit-body productivity and inter single sequence repeat (ISSR) marker profiling. Hybrid population was assessed with different phenotypic variables by one-way analysis of variance. Principal component matrices were analyzed for the six phenotypic variables in scatter plot showing maximum positive correlation between each variable for all strains examined. Six ISSR primers generated 66 reproducible fragments with 98.48 % polymorphism. The dendrogram thus created based on unweighted pair-group method with mathematic averages method of clustering and Euclidean distance which exhibited three major groups between the parents and pfle hybrids. Though P. florida parent remained in one group but it showed different degrees of genetic distance with all the hybrid lines belonging to the other two groups while L. edodes was most distantly related to all the hybrid lines. L. edodes specific sequence-rich ISSR amplicon was recorded in all the hybrid lines and in L. edodes but not in P. florida. All the fruit body generating pfle hybrid lines could produce basidiocarp on paddy straw in sub-tropical climate and showed phenotypic resemblance to the P. florida parent.

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Abbreviations

ANOVA:

Analysis of variance

FDA:

Fluorescein diacetate

IOA:

Iodoacetamide

ISSR:

Inter single sequence repeat

MYG:

Malt-yeast extract-glucose

MYGNa:

MYG supplemented with 0.7 M NaCl

PDA:

Potato-dextrose-agar

PCA:

Principal component analysis

PIC:

Polymorphic information content

SCAR:

Sequence characterized amplified region

UPGMA:

Unweighted pair-group method with mathematic averages

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Acknowledgments

We acknowledge fellowship support to the first author from Bose Institute, Kolkata. We also thankful to Dr. Somnath Bhattacharya, Reader, Bidhan Chandra Krishi Viswa Vidyalaya, Mohanpur, Nadia, West Bengal for his help in statistical analysis.

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Authors and Affiliations

  1. Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata, 700 054, West Bengal, India

    Pijush Mallick & Samir Ranjan Sikdar

Authors
  1. Pijush Mallick
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  2. Samir Ranjan Sikdar
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Corresponding author

Correspondence to Samir Ranjan Sikdar.

Electronic supplementary material

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11274_2014_1652_MOESM1_ESM.tif

Supplementary Figure 1 Dendrogram showing the relationship amongst the somatic hybrid lines and their parents using (i) Genetic Divergence obtained from DNA fingerprints of 6 ISSR primers and the (ii) UPGMA method of clustering and Euclidean distance. (TIFF 176 kb)

11274_2014_1652_MOESM2_ESM.doc

Supplementary Table 1 Proximity matrix of Jaccard’s dissimilarity coefficients based on amplified DNA fragments from six ISSR primers amongst the pfle hybrid lines and their parents (matrix input file). (DOC 53 kb)

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Mallick, P., Sikdar, S.R. Production and molecular characterization of somatic hybrids between Pleurotus florida and Lentinula edodes . World J Microbiol Biotechnol 30, 2283–2293 (2014). https://doi.org/10.1007/s11274-014-1652-x

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