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Lactonase-expressing Lactobacillus plantarum NC8 attenuates the virulence factors of multiple drug resistant Pseudomonas aeruginosa in co-culturing environment

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Abstract

Pseudomonas aeruginosa possesses an arcade of both cell-associated and extracellular cytotoxic virulence factors which are regulated by a multi-component quorum sensing system. Many research studies report success of lactonase in combating the pathogenicity of P. aeruginosa but delivery of lactonase remains a challenge. The present study aims at developing a delivery vehicle for lactonase. Lactobacillus plantarum NC8 was used as host for aiiA (Bacillus thuringiensis 4A3 lactonase gene) using pSIP409 expression vector. pSIP409: aiiA construct was stably maintained in L. plantarum NC8. Co-culturing of multi-drug resistant (MDR) clinical isolates of P. aeruginosa and PAO1 with recombinant L. plantarum NC8 led to significant reduction (p < 0.001) in extracellular virulence factors like pyocyanin, protease, elastase and rhamnolipids in P. aeruginosa and also showed significant reduction in adhesion of P. aeruginosa strains to uroepithelial cells in vitro. This study shows the heterologous expression of AiiA lactonase in L. plantarum NC8. Co-culturing of lactonase expressing L. plantarum NC8 with MDR P. aeruginosa strains led to attenuation of their virulence significantly. These results underscore the potential application of recombinant L. plantarum NC8 with anti-quorum sensing properties to control infections caused by multidrug resistant P. aeruginosa.

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Acknowledgments

The authors thank Dr. Deborah A. Hogan, Department of Microbiology and Immunology, Dartmouth Medical School, New Hampshire, USA for providing the biosensor strain, A. tumefaciens NT1, Dr. Vittorio Venturi, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy for providing C. violaceum CV026, Dr. Walter Fast, University of Texas, College of Pharmacy (Austin, Texas) for providing the plasmid pMAL-c2x-t containing aiiA gene and Dr. Lars Axelsson, Microbial Physiology and Genetics, Technical University of Denmark, Denmark for providing the plasmid pSIP409, induction peptide, and L. plantarum NC8. The present study was funded by Indian Council of Medical Research, New Delhi, India.

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Authors and Affiliations

  1. Department of Biotechnology, Panjab University, Chandigarh, 160014, India

    Sudha Joshi, Amanjot Kaur & Neena Capalash

  2. Department of Microbiology, Panjab University, Chandigarh, 160014, India

    Prince Sharma & Kusum Harjai

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  1. Sudha Joshi
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Correspondence to Neena Capalash.

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Joshi, S., Kaur, A., Sharma, P. et al. Lactonase-expressing Lactobacillus plantarum NC8 attenuates the virulence factors of multiple drug resistant Pseudomonas aeruginosa in co-culturing environment. World J Microbiol Biotechnol 30, 2241–2249 (2014). https://doi.org/10.1007/s11274-014-1645-9

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