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Diversity of Rhizoctonia solani associated with pulse crops in different agro-ecological regions of India

  • Sunil C. DubeyEmail author
  • Aradhika Tripathi
  • Balendu K. Upadhyay
  • Utpal K. Deka
Original Paper

Abstract

Four hundred seventy Rhizoctonia solani isolates from different leguminous hosts originating from 16 agro-ecological regions of India covering 21 states and 72 districts were collected. The disease incidence caused by R. solani varied from 6.8 to 22.2 % in the areas surveyed. Deccan plateau and central highlands, hot sub-humid ecoregion followed by northern plain and central highlands and hot semi-arid ecoregion showed the highest disease incidence. R. solani isolates were highly variable in growth diameter, number, size and pattern of sclerotia formation as well as hyphal width. The isolates obtained from aerial part of the infected plants showing web blight symptoms produced sclerotia of 1–2 mm in size whereas, the isolates obtained from infected root of the plants showing wet root rot symptoms produced microsclerotia (<1 mm). Majority of R. solani isolates showed <8 μm hyphal diameter. Based on morphological characters the isolates were categorized into 49 groups. Seven anastomosis groups (AGs) were identified among the populations of R. solani associated with the pulse crops. The frequency (25.6 %) of AG3 was the highest followed by AG2–3 (20.9 %) and AG5 (17.4 %). The cropping sequence of rice/sorghum/wheat-chickpea/mungbean/urdbean/cowpea/ricebean influenced the dominance of AG1 (16.3 %). Phylogenetic analysis utilizing ITS-5.8S rDNA gene sequences indicated high level of genetic similarity among isolates representing different AGs, crops and regions. ITS groups did not correspond to the morphological characters. The sequence data from this article has been deposited with NCBI data libraries with JF701707 to JF701795 accession numbers.

Keywords

Rhizoctonia solani Morphology Anastomosis ITS sequencing Agro-ecological regions Pulse crops 

Notes

Acknowledgments

Authors are thankful to Indian Council of Agricultural Research, New Delhi for financial support and Dr. H. Nirenberg, Julius Kühn- Institute (BBA), Germany and Dr. Mitsuro Hyakumachi, Gifu University, Japan for providing the international tester isolates of various AGs. Divisional publication accession no.4/12.

References

  1. Abd-Elsalam AK, Moslem MA, Bahkali AH (2009) First morpho-molecular identification of Rhizoctonia solani AG-7 from potato tuber-borne sclerotium in Saudi Arabia. Afr J Microbiol Res 3:952–956Google Scholar
  2. Agricultural Statistics at a Glance (2011) Directorate of Economics and Statistics, Department of Agriculture, Ministery of Agriculture, Goverment of IndiaGoogle Scholar
  3. Ahvenniemi P, Wolf M, Lehtonen MJ, Wilson P, German-Kinnari M, Valkonen JP (2009) Evolutionary diversification indicated by compensatory base changes in ITS2 secondary structures in a complex fungal species, Rhizoctonia solani. J Mol Evol 69:150–163CrossRefGoogle Scholar
  4. Andersen TF (1990) A study of hyphal morphology in the form genus Rhizoctonia. Mycotaxon 37:25–46Google Scholar
  5. Bandoni RJ (1979) Safranin-O as a rapid nuclear stain for fungi. Mycologia 71:873–874CrossRefGoogle Scholar
  6. Bridge PD, Arora DK, Reddy CA (1998) Application of PCR in mycology. Cab International, New YorkGoogle Scholar
  7. Brinboim HC, Dolly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523CrossRefGoogle Scholar
  8. Carling DE (1996) Grouping in Rhizoctonia solani by hyphal anastomosis. In: Sneh B, Jabaji-Hare S, Neate S, Dijst G (eds) Rhizoctonia species: taxonomy, molecular biology, ecology, pathology, and disease control. Kluwer, Dordrecht, pp 37–47CrossRefGoogle Scholar
  9. Carling DE, Baird RE, Gitaitis RD, Brained KA, Kuninaga S (2002a) Characterization of AG-13, a newly reported anastomosis group of Rhizoctonia solani. Dis Cont Pest Manag 92:893–899Google Scholar
  10. Carling DE, Kuninaga S, Brainard KA (2002b) Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology 92:43–50CrossRefGoogle Scholar
  11. Cebi Kilicoglu M, Ozkoc I (2008) Molecular developments in fungal systematics. J Agric Fac 23:65–72Google Scholar
  12. Ciampi MB, Meyer MC, Costa MJN, Zala M, McDonald BA, Ceresini PC (2008) Genetic structure of populations of Rhizoctonia solani anastomosis group-1 IA from soybean in Brazil. Phytopathology 98:932–941CrossRefGoogle Scholar
  13. Cochran WG (1977) Sampling techniques, 3rd edn. John Wiley and Sons, New York, p 428Google Scholar
  14. Dubey SC (2003) Integrated management of web blight of urd/mung bean by bio-seed treatment. Indian Phytopath 56:34–38Google Scholar
  15. Dubey SC, Dwivedi RP (1991) Reaction of groundnut germplasms to Thanatephorus cucumeris (Fr.) Donk. J Res (BAU) 3:97–98Google Scholar
  16. Fiers M, Edel-Hermann V, Héraud C, Gautheron N, Chatot C, Le Hingrat Y, Bouchek-Mechiche K, Steinberg C (2011) Genetic diversity of Rhizoctonia solani associated with potato tubers in France. Mycologia 103:1230–1244CrossRefGoogle Scholar
  17. Godoy-Lutz G, Kuninaga S, Steadman JR, Powers K (2008) Phylogenetic analysis of Rhizoctonia solani subgroups associated with web blight symptoms on common bean based on ITS-5.8S rDNA. J Gen Plant Pathol 74:32–40CrossRefGoogle Scholar
  18. Gomez KA, Gomez AA (1984) Statistical procedure for agricultural research. John Wiley and Sons, Singapore, pp 139–153Google Scholar
  19. Gonzalez Garcia V, Portal OMA, Rubio SV (2006) Review, biology and systematics of the form genus Rhizoctonia. Span J Agric Res 4:55–79Google Scholar
  20. Gonzalez D, Carling DE, Kuninaga S, Vilgalys R, Cubeta MA (2001) Ribosomal DNA systematics of Ceratobasidium and Thanatephorus with Rhizoctonia anamorphs. Mycologia 93:1138–1150CrossRefGoogle Scholar
  21. Goswami BK, Bhuiyan KA, Mian IH (2008) Cultural and morphological variation in the isolates of Rhizoctonia solani. Bangl J Plant Pathol 24:63–68CrossRefGoogle Scholar
  22. Grosch R, Schneider JHM, Kofoet A (2004) Characterization of Rhizoctonia solani anastomosis groups causing bottom rot in fieldgrown lettuce in Germany. Eur J Plant Pathol 110:53–62CrossRefGoogle Scholar
  23. Hyakumachia M, Mushikaa T, Ogisoa Y, Todaa T, Kageyamaa K, Tsugeb T (1998) Characterization of a new cultural type (LP) of Rhizoctonia solani AG2-2 isolated from warm-season turfgrasses, and its genetic differentiation from other cultural types. Plant Pathol 47:1–9CrossRefGoogle Scholar
  24. Kanematsu S, Naito S (1994) Genetic characterization of Rhizoctonia solani AG2-3 by analyzing restriction fragment length polymorphisms of nuclear ribosomal DNA internal transcribed spacers. Ann Phytopathol Soc Jpn 61:18–21CrossRefGoogle Scholar
  25. KeMei L, Guo QY, Zhao L, Chen XZ (2009) Study on the anastomosis groups of Rhizoctonia solani isolated from lucerne in Xinjiang and their pathogenicity. Pharm Sci 26:151–154Google Scholar
  26. Kilicoglu MC, Ozkoc I (2010) Molecular characterization of Rhizoctonia solani AG4 using PCR-RFLP of the rDNA-ITS region. Turk J Biol 34:261–269Google Scholar
  27. Kronland WC, Stanghellini ME (1988) Clean slide technique for the observation of anastomosis and nuclear condition of Rhizoctonia solani. Phytopathology 78:820–822CrossRefGoogle Scholar
  28. Lilly VG, Barnett HL (1951) Physiology of fungi. Mc Graw Hill Book, New York, p 463Google Scholar
  29. Liu ZL, Domier LL, Sinclair JB (1995) Polymorphism of genes coding for nuclear 18S rDNA indicates genetic distinctiveness of anastomosis group 10 from other groups in the Rhizoctonia solani species complex. Appl Environ Microbiol 61:2659–2664Google Scholar
  30. Mendal M, Higa A (1970) Calcium dependent bacteriophase DNA isolation. J Mol Biol 53:159–162CrossRefGoogle Scholar
  31. Mikhail MS, Sabet KK, Omar MR, Amal AA, Kasem KK (2010) Current Rhizoctonia solani anastomosis groups in Egypt and their pathogenic relation to cotton seedlings. Afr J Microbiol Res 4:386–395Google Scholar
  32. Murray MG, Thompson WE (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325CrossRefGoogle Scholar
  33. Ogoshi A (1972) Grouping of Rhizoctonia solani Kühn with hyphal anastomosis. Ann Phytopathol Soc Jpn 38:117–122CrossRefGoogle Scholar
  34. Ogoshi A (1987) Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kühn. Annu Rev Phytopathol 25:125–143CrossRefGoogle Scholar
  35. Parmeter JR Jr (1970) Rhizoctonia solani: biology and pathology. University of California Press, Berkeley CAGoogle Scholar
  36. Parmeter JR Jr, Whiteny HS (1970) Taxonomy and nomenclature of the imperfect state. In: Parmeter JR Jr (ed) Rhizoctonia solani: biology and pathology. California press, Berkeley, pp 20–31Google Scholar
  37. Parmeter JR Jr, Sherwood RT, Platt WD (1969) Anastomosis grouping among isolates of Thanatephorus cucumeris. Phytopathology 59:1270–1278Google Scholar
  38. Saksena HK, Dwivedi RP (1973) Web blight of blackgram caused by Thanatephorus cucumeris. Indian J Farm Sci 1:58–61Google Scholar
  39. Saksena HK, Vaartaja O (1961) Taxonomy, morphology and pathogenicity of Rhizoctonia species from forest nurseries. Can J Bot 39:627–647CrossRefGoogle Scholar
  40. Sambrook JE, Fritsch F, Maniatis T (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
  41. Sherwood RT (1969) Morphology and physiology in four anastomosis groups of Thanatephorus cucumeris. Phytopathology 59:1924–1929Google Scholar
  42. Sneh B, Burpee L, Ogoshi A (1991) Identification of Rhizoctonia species. APS Press, USA, p 133Google Scholar
  43. Talbot PHB (1970) Taxonomy and nomenclature of the imperfect state. In: Parmeter JR Jr (ed) Rhizoctonia solani: biology and pathology. California press, Berkeley, pp 7–19Google Scholar
  44. Tiwari A, Khare MN, Tiwari A (1998) Variability among isolates of Rhizoctonia solani infecting mungbean. Indian Phytopathol 51:334–337Google Scholar
  45. Vishwadhar, Chaudhary RG (2001) Disease resistance in pulse crops-current status and future approaches. In: Nagarajan S, Singh DP (eds) The role of resistance in intensive agriculture. Kalyani, New Delhi, pp 144–157Google Scholar
  46. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninisky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar
  47. You MP, Lancaster B, Sivasithamparam K, Barbetti MJ (2008) Cross-pathogenicity of Rhizoctonia solani strains on pasture legumes in pasture-crop rotations. Plant Soil 302:203–211CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Sunil C. Dubey
    • 1
    Email author
  • Aradhika Tripathi
    • 1
  • Balendu K. Upadhyay
    • 1
  • Utpal K. Deka
    • 1
  1. 1.Division of Plant PathologyIndian Agricultural Research InstituteNew DelhiIndia

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