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Heat treatment induced bacterial changes in irrigation water and their implications for plant disease management

  • W. Hao
  • C. X. Hong
Original Paper

Abstract

A new heat treatment for recycled irrigation water using 48 °C for 24 h to inactivate Phytophthora and bacterial plant pathogens is estimated to reduce fuel cost and environmental footprint by more than 50 % compared to current protocol (95 °C for 30 s). The objective of this study was to determine the impact of this new heat treatment temperature regime on bacterial community structure in water and its practical implications. Bacterial communities in irrigation water were analyzed before and after heat treatment using both culture-dependent and -independent strategies based on the 16S ribosomal DNA. A significant shift was observed in the bacterial community after heat treatment. Most importantly, bacteria with biological control potential—Bacillus and Paenibacillus, and Pseudomonas species became more abundant at both 48 and 42 °C. These findings imply that the new heat treatment procedure not only controls existing plant pathogens but also may make the heat-treated irrigation water a more antagonistic environment against plant pathogens, promoting sustainable disease management.

Keywords

Biological control activity Bacillus Colony PCR–SSCP Paenibacillus PCR–DGGE Pseudomonas 

Notes

Acknowledgments

This work was supported by a grant from the USDA National Institute of Food and Agriculture—Specialty Crop Research Initiative (Agreement #: 2010-51181-21140). We would like to thank Drs. Boris Vinatzer, Anton Baudoin, Erik Stromberg, Michael Benson, Giovanni Cafà, and Ping Kong for their valuable advice during this study, and we also would like to thank Patricia Richardson for assisting with water sampling and proofreading this manuscript.

Supplementary material

11274_2013_1583_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)
11274_2013_1583_MOESM2_ESM.docx (36 kb)
Supplementary material 2 (DOCX 36 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Hampton Roads Agricultural Research and Extension CenterVirginia TechVirginia BeachUSA

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