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Effect of copper-induced oxidative stress on sclerotial differentiation and antioxidant properties of Penicillium thomii PT95 strain

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Abstract

Penicillium thomii PT95 strain was able to form abundant orange, sand-shaped sclerotia in which carotenoids were accumulated. The aim of this work was to determine the effects of copper-induced oxidative stress on the sclerotial differentiation and antioxidant properties of PT95 strain. The results showed that the time of exudates initiation, sclerotial initiation and sclerotial maturation of PT95 strain were advanced in 1–2 days under the copper-induced oxidative stress growth conditions. The analytical results of sclerotial biomass, carotenoids content in sclerotia showed that copper-induced oxidative stress favored the sclerotial differentiation and biosynthesis of carotenoids. Under the copper-induced oxidative stress growth conditions, the total phenolics content and DPPH free radical scavenging activity of sclerotia of this fungus were decreased as compared with the control. However, the oxidative stress induced by a lower amount of CuSO4 in media could enhance significantly the reducing power of sclerotia.

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Acknowledgments

Support for this research by the Chinese National Natural Science Fund (Grant No. 31070048) is gratefully acknowledged.

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Authors and Affiliations

  1. School of Life Science, Shanxi University, Taiyuan, China

    Lin-Ru Niu & Jian-Rong Han

  2. Institute of Biotechnology, Shanxi University, Taiyuan, China

    Ze-Qing Zhang & Dan-Dan Long

  3. Taiyuan Normal University, Taiyuan, China

    Wen-Jing Zhao

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  1. Ze-Qing Zhang
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  2. Wen-Jing Zhao
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  3. Dan-Dan Long
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  4. Lin-Ru Niu
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Correspondence to Jian-Rong Han.

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Zhang, ZQ., Zhao, WJ., Long, DD. et al. Effect of copper-induced oxidative stress on sclerotial differentiation and antioxidant properties of Penicillium thomii PT95 strain. World J Microbiol Biotechnol 30, 1519–1525 (2014). https://doi.org/10.1007/s11274-013-1572-1

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  • DOI: https://doi.org/10.1007/s11274-013-1572-1

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